Root-mediated bacterial accessibility and cometabolism of pyrene in soil

Fernández-López, Carmen; Posada Baquero, Rosa; García Fernández, José Luis; Castilla-Alcántara, J. C.; Cantos, Manuel; Ortega Calvo, J. J.; Ministerio de Economía y Competitividad (España)

Root-mediated bacterial accessibility and cometabolism of pyrene in soil

13 páginas.-- 6 figuras.- 1 tablas.- 43 referencias.- Supplementary data to this article can be found online at https://doi.org/10.1016/j.scitotenv.2020.143408

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Main Authors:	Fernández-López, Carmen, Posada Baquero, Rosa, García Fernández, José Luis, Castilla-Alcántara, J. C., Cantos, Manuel, Ortega Calvo, J. J.
Other Authors:	Ministerio de Economía y Competitividad (España)
Format:	artículo biblioteca
Language:	English
Published:	Elsevier 2021-03-15
Subjects:	Bacteria, Biodegradation, Bioremediation, Polycyclic aromatic hydrocarbons, Sunflower,
Online Access:	http://hdl.handle.net/10261/224354 http://dx.doi.org/10.13039/501100004837 http://dx.doi.org/10.13039/501100003329
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record_format	koha
spelling	dig-irnas-es-10261-2243542021-03-19T08:14:44Z Root-mediated bacterial accessibility and cometabolism of pyrene in soil Fernández-López, Carmen Posada Baquero, Rosa García Fernández, José Luis Castilla-Alcántara, J. C. Cantos, Manuel Ortega Calvo, J. J. Ministerio de Economía y Competitividad (España) Ministerio de Ciencia e Innovación (España) Fernández-López, Carmen [0000-0003-2868-0231] Posada Baquero, Rosa [0000-0003-2726-7357] García Fernández, José Luis [0000-0001-7880-2343] Castilla-Alcántara, J. C. [0000-0002-9123-1052] Cantos, Manuel [0000-0001-8672-4437] Ortega Calvo, J. J. [0000-0003-1672-5199] Bacteria Biodegradation Bioremediation Polycyclic aromatic hydrocarbons Sunflower 13 páginas.-- 6 figuras.- 1 tablas.- 43 referencias.- Supplementary data to this article can be found online at https://doi.org/10.1016/j.scitotenv.2020.143408 Partial transformation of pollutants and mobilization of the produced metabolites may contribute significantly to the risks resulting from biological treatment of soils polluted by hydrophobic chemicals such as polycyclic aromatic hydrocarbons (PAHs). Pyrene, a four-ringed PAH, was selected here as a model pollutant to study the effects of sunflower plants on the bacterial accessibility and cometabolism of this pollutant when located at a spatially distant source within soil. We compared the transformation of passively dosed 14C-labeled pyrene in soil slurries and planted pots that were inoculated with the bacterium Pseudomonas putida G7. This bacterium combines flagellar cell motility with the ability to cometabolically transform pyrene. Cometabolism of this PAH occurred immediately in the inoculated and shaken soil slurries, where the bacteria had full access to the passive dosing devices (silicone O-rings). Root exudates did not enhance the survival of P. putida G7 cells in soil slurries, but doubled their transport in column tests. In greenhouse-incubated soil pots with the same pyrene sources instead located centimeters from the soil surface, the inoculated bacteria transformed 14C-labeled pyrene only when the pots were planted with sunflowers. Bacterial inoculation caused mobilization of 14C-labeled pyrene metabolites into the leachates of the planted pots at concentrations of approximately 1 mg L−1, ten times greater than the water solubility of the parent compound. This mobilization resulted in a doubled specific root uptake rate of 14C-labeled pyrene equivalents and a significantly decreased root-to-fruit transfer rate. Our results show that the plants facilitated bacterial access to the distant pollutant source, possibly by increasing bacterial dispersal in the soil; this increased bacterial access was associated with cometabolism, which contributed to the risks of biodegradation. © 2020 Elsevier B.V. We thank the Spanish Ministries of Economy and Competitiveness ( CGL2016-77497-R ) and Science and Innovation ( PID2019-109700RB-C21 ) for supporting this work. Peer reviewed 2020-12-04T07:59:19Z 2020-12-04T07:59:19Z 2021-03-15 artículo http://purl.org/coar/resource_type/c_6501 Science of The Total Environment (760): 143408 (2021) 0048-9697 http://hdl.handle.net/10261/224354 10.1016/j.scitotenv.2020.143408 http://dx.doi.org/10.13039/501100004837 http://dx.doi.org/10.13039/501100003329 en #PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/CGL2016-77497-R info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-109700RB-C21 Postprint http://dx.doi.org/10.1016/j.scitotenv.2020.143408 Sí embargo_20230315 Elsevier
institution	IRNAS ES
collection	DSpace
country	España
countrycode	ES
component	Bibliográfico
access	En linea
databasecode	dig-irnas-es
tag	biblioteca
region	Europa del Sur
libraryname	Biblioteca del IRNAS España
language	English
topic	Bacteria Biodegradation Bioremediation Polycyclic aromatic hydrocarbons Sunflower Bacteria Biodegradation Bioremediation Polycyclic aromatic hydrocarbons Sunflower
spellingShingle	Bacteria Biodegradation Bioremediation Polycyclic aromatic hydrocarbons Sunflower Bacteria Biodegradation Bioremediation Polycyclic aromatic hydrocarbons Sunflower Fernández-López, Carmen Posada Baquero, Rosa García Fernández, José Luis Castilla-Alcántara, J. C. Cantos, Manuel Ortega Calvo, J. J. Root-mediated bacterial accessibility and cometabolism of pyrene in soil
description	13 páginas.-- 6 figuras.- 1 tablas.- 43 referencias.- Supplementary data to this article can be found online at https://doi.org/10.1016/j.scitotenv.2020.143408
author2	Ministerio de Economía y Competitividad (España)
author_facet	Ministerio de Economía y Competitividad (España) Fernández-López, Carmen Posada Baquero, Rosa García Fernández, José Luis Castilla-Alcántara, J. C. Cantos, Manuel Ortega Calvo, J. J.
format	artículo
topic_facet	Bacteria Biodegradation Bioremediation Polycyclic aromatic hydrocarbons Sunflower
author	Fernández-López, Carmen Posada Baquero, Rosa García Fernández, José Luis Castilla-Alcántara, J. C. Cantos, Manuel Ortega Calvo, J. J.
author_sort	Fernández-López, Carmen
title	Root-mediated bacterial accessibility and cometabolism of pyrene in soil
title_short	Root-mediated bacterial accessibility and cometabolism of pyrene in soil
title_full	Root-mediated bacterial accessibility and cometabolism of pyrene in soil
title_fullStr	Root-mediated bacterial accessibility and cometabolism of pyrene in soil
title_full_unstemmed	Root-mediated bacterial accessibility and cometabolism of pyrene in soil
title_sort	root-mediated bacterial accessibility and cometabolism of pyrene in soil
publisher	Elsevier
publishDate	2021-03-15
url	http://hdl.handle.net/10261/224354 http://dx.doi.org/10.13039/501100004837 http://dx.doi.org/10.13039/501100003329
work_keys_str_mv	AT fernandezlopezcarmen rootmediatedbacterialaccessibilityandcometabolismofpyreneinsoil AT posadabaquerorosa rootmediatedbacterialaccessibilityandcometabolismofpyreneinsoil AT garciafernandezjoseluis rootmediatedbacterialaccessibilityandcometabolismofpyreneinsoil AT castillaalcantarajc rootmediatedbacterialaccessibilityandcometabolismofpyreneinsoil AT cantosmanuel rootmediatedbacterialaccessibilityandcometabolismofpyreneinsoil AT ortegacalvojj rootmediatedbacterialaccessibilityandcometabolismofpyreneinsoil
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Root-mediated bacterial accessibility and cometabolism of pyrene in soil

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