Definition of a novel symbiovar (sv. retamae) within Bradyrhizobium retamae sp. nov., nodulating Retama sphaerocarpa and Retama monosperma
In this paper we analyze through a polyphasic approach several Bradyrhizobium strains isolated in Spain and Morocco from root nodules of Retama sphaerocarpa and Retama monosperma. All the strains have identical 16S rRNA genes and their closest relative species is Bradyrhizobium lablabi CCBAU 23086T, with 99.41% identity with respect to the strain Ro19T. Despite the closeness of the 16S rRNA genes, the housekeeping genes recA, atpD and glnII were divergent in Ro19T and B. lablabi CCBAU 23086T, with identity values of 95.71%, 93.75% and 93.11%, respectively. These differences were congruent with DNA-DNA hybridization analysis that revealed an average of 35% relatedness between the novel species and B. lablabi CCBAU 23086T. Also, differential phenotypic characteristics of the new species were found with respect to the already described species of Bradyrhizobium. Based on the genotypic and phenotypic data obtained in this study, we propose to classify the group of strains isolated from R. sphaerocarpa and R. monosperma as a novel species named Bradyrhizobium retamae sp. nov. (type strain Ro19T=LMG 27393T=CECT 8261T). The analysis of symbiotic genes revealed that some of these strains constitute a new symbiovar within genus Bradyrhizobium for which we propose the name >retamae>, that mainly contains nodulating strains isolated from Retama species in different continents.
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Elsevier BV
2013-06
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Subjects: | Bradyrhizobium retamae, Retama, Phylogeny, Symbiovar, |
Online Access: | http://hdl.handle.net/10261/199077 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100003329 http://dx.doi.org/10.13039/501100004837 http://dx.doi.org/10.13039/100012818 http://dx.doi.org/10.13039/501100003339 http://dx.doi.org/10.13039/501100011011 http://dx.doi.org/10.13039/501100011698 |
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dig-irnas-es-10261-1990772021-06-10T14:52:36Z Definition of a novel symbiovar (sv. retamae) within Bradyrhizobium retamae sp. nov., nodulating Retama sphaerocarpa and Retama monosperma Guerrouj, Kamal Ruiz Díez, Beatriz Chahboune, Rajaa Ramírez-Bahena, Marta-Helena Abdelmoumen, H. Quiñones, Miguel Angel Missbah El Idrissi, M. Velázquez, Encarna Fernández-Pascual, Mercedes Bedmar, Eulogio J. Peix, Álvaro European Commission Junta de Andalucía Junta de Comunidades de Castilla-La Mancha Comunidad de Madrid Ministerio de Economía y Competitividad (España) Ministerio de Ciencia e Innovación (España) Consejo Superior de Investigaciones Científicas (España) Bradyrhizobium retamae Retama Phylogeny Symbiovar In this paper we analyze through a polyphasic approach several Bradyrhizobium strains isolated in Spain and Morocco from root nodules of Retama sphaerocarpa and Retama monosperma. All the strains have identical 16S rRNA genes and their closest relative species is Bradyrhizobium lablabi CCBAU 23086T, with 99.41% identity with respect to the strain Ro19T. Despite the closeness of the 16S rRNA genes, the housekeeping genes recA, atpD and glnII were divergent in Ro19T and B. lablabi CCBAU 23086T, with identity values of 95.71%, 93.75% and 93.11%, respectively. These differences were congruent with DNA-DNA hybridization analysis that revealed an average of 35% relatedness between the novel species and B. lablabi CCBAU 23086T. Also, differential phenotypic characteristics of the new species were found with respect to the already described species of Bradyrhizobium. Based on the genotypic and phenotypic data obtained in this study, we propose to classify the group of strains isolated from R. sphaerocarpa and R. monosperma as a novel species named Bradyrhizobium retamae sp. nov. (type strain Ro19T=LMG 27393T=CECT 8261T). The analysis of symbiotic genes revealed that some of these strains constitute a new symbiovar within genus Bradyrhizobium for which we propose the name >retamae>, that mainly contains nodulating strains isolated from Retama species in different continents. The authors acknowledge funding from ERDF-cofinanced grant P09RNM4746 from Consejería de Economía, Innovación y Ciencia (Junta de Andalucía), Junta de Castilla y León, Junta de Castilla-La Mancha and Comunidad de Madrid (Regional Spanish Governments), MINECO and MCI (Central Spanish Government). BRD was supported by contract of Junta de Comunidades de Castilla La Mancha (Project ref. POII10-0211-5015). MHRB is recipient of a JAE-Doc researcher contract from CSIC. The authors thank S. Fajardo for excellent technical assistance and Dr. J.P. Euzeby for his help with the naming of the novel species. 2020-01-28T12:37:13Z 2020-01-28T12:37:13Z 2013-06 2020-01-28T12:37:13Z artículo http://purl.org/coar/resource_type/c_6501 doi: 10.1016/j.syapm.2013.03.001 issn: 0723-2020 Systematic and Applied Microbiology 36(4): 218-223 (2013) http://hdl.handle.net/10261/199077 10.1016/j.syapm.2013.03.001 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100003329 http://dx.doi.org/10.13039/501100004837 http://dx.doi.org/10.13039/100012818 http://dx.doi.org/10.13039/501100003339 http://dx.doi.org/10.13039/501100011011 http://dx.doi.org/10.13039/501100011698 http://dx.doi.org/10.1016/j.syapm.2013.03.001 Sí none Elsevier BV |
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Bradyrhizobium retamae Retama Phylogeny Symbiovar Bradyrhizobium retamae Retama Phylogeny Symbiovar |
spellingShingle |
Bradyrhizobium retamae Retama Phylogeny Symbiovar Bradyrhizobium retamae Retama Phylogeny Symbiovar Guerrouj, Kamal Ruiz Díez, Beatriz Chahboune, Rajaa Ramírez-Bahena, Marta-Helena Abdelmoumen, H. Quiñones, Miguel Angel Missbah El Idrissi, M. Velázquez, Encarna Fernández-Pascual, Mercedes Bedmar, Eulogio J. Peix, Álvaro Definition of a novel symbiovar (sv. retamae) within Bradyrhizobium retamae sp. nov., nodulating Retama sphaerocarpa and Retama monosperma |
description |
In this paper we analyze through a polyphasic approach several Bradyrhizobium strains isolated in Spain and Morocco from root nodules of Retama sphaerocarpa and Retama monosperma. All the strains have identical 16S rRNA genes and their closest relative species is Bradyrhizobium lablabi CCBAU 23086T, with 99.41% identity with respect to the strain Ro19T. Despite the closeness of the 16S rRNA genes, the housekeeping genes recA, atpD and glnII were divergent in Ro19T and B. lablabi CCBAU 23086T, with identity values of 95.71%, 93.75% and 93.11%, respectively. These differences were congruent with DNA-DNA hybridization analysis that revealed an average of 35% relatedness between the novel species and B. lablabi CCBAU 23086T. Also, differential phenotypic characteristics of the new species were found with respect to the already described species of Bradyrhizobium. Based on the genotypic and phenotypic data obtained in this study, we propose to classify the group of strains isolated from R. sphaerocarpa and R. monosperma as a novel species named Bradyrhizobium retamae sp. nov. (type strain Ro19T=LMG 27393T=CECT 8261T). The analysis of symbiotic genes revealed that some of these strains constitute a new symbiovar within genus Bradyrhizobium for which we propose the name >retamae>, that mainly contains nodulating strains isolated from Retama species in different continents. |
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European Commission |
author_facet |
European Commission Guerrouj, Kamal Ruiz Díez, Beatriz Chahboune, Rajaa Ramírez-Bahena, Marta-Helena Abdelmoumen, H. Quiñones, Miguel Angel Missbah El Idrissi, M. Velázquez, Encarna Fernández-Pascual, Mercedes Bedmar, Eulogio J. Peix, Álvaro |
format |
artículo |
topic_facet |
Bradyrhizobium retamae Retama Phylogeny Symbiovar |
author |
Guerrouj, Kamal Ruiz Díez, Beatriz Chahboune, Rajaa Ramírez-Bahena, Marta-Helena Abdelmoumen, H. Quiñones, Miguel Angel Missbah El Idrissi, M. Velázquez, Encarna Fernández-Pascual, Mercedes Bedmar, Eulogio J. Peix, Álvaro |
author_sort |
Guerrouj, Kamal |
title |
Definition of a novel symbiovar (sv. retamae) within Bradyrhizobium retamae sp. nov., nodulating Retama sphaerocarpa and Retama monosperma |
title_short |
Definition of a novel symbiovar (sv. retamae) within Bradyrhizobium retamae sp. nov., nodulating Retama sphaerocarpa and Retama monosperma |
title_full |
Definition of a novel symbiovar (sv. retamae) within Bradyrhizobium retamae sp. nov., nodulating Retama sphaerocarpa and Retama monosperma |
title_fullStr |
Definition of a novel symbiovar (sv. retamae) within Bradyrhizobium retamae sp. nov., nodulating Retama sphaerocarpa and Retama monosperma |
title_full_unstemmed |
Definition of a novel symbiovar (sv. retamae) within Bradyrhizobium retamae sp. nov., nodulating Retama sphaerocarpa and Retama monosperma |
title_sort |
definition of a novel symbiovar (sv. retamae) within bradyrhizobium retamae sp. nov., nodulating retama sphaerocarpa and retama monosperma |
publisher |
Elsevier BV |
publishDate |
2013-06 |
url |
http://hdl.handle.net/10261/199077 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100003329 http://dx.doi.org/10.13039/501100004837 http://dx.doi.org/10.13039/100012818 http://dx.doi.org/10.13039/501100003339 http://dx.doi.org/10.13039/501100011011 http://dx.doi.org/10.13039/501100011698 |
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