Alterations in Mediterranean mussel (Mytilus galloprovincialis) composition exposed to cyanotoxins as revealed by nalytical pyrolysis
Póster presentado en el 1st Iberian Meeting in Separation Sciences & Mass Spectrometry, XIX Conference of the Spanish Society of Chromatography and Related Techniques (SECyTA), IX Conference of the Spanish Society of Mass Spectrometry (SEEM), VI Conference of the Mass Spectrometry Group of the Portuguese Society of Chemistry (SPQ). Santiago de Compostela, October 8th-11th (2019).
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Sociedad Española de Cromatografía y Técnicas Afines
2019-10
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Online Access: | http://hdl.handle.net/10261/192806 http://dx.doi.org/10.13039/501100003329 http://dx.doi.org/10.13039/501100000780 |
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dig-irnas-es-10261-1928062020-10-07T11:18:58Z Alterations in Mediterranean mussel (Mytilus galloprovincialis) composition exposed to cyanotoxins as revealed by nalytical pyrolysis Diez‐Quijada, Leticia de Oliveira, Flavio L. Jos, A. Cameán Fernández, A. M. Aparicio-Ruiz, R. Vasconcelos, Vítor Campos, Alexandre Oliveira González-Vila, Francisco Javier González-Pérez, José Antonio Ministerio de Economía y Competitividad (España) European Commission González-Vila, Francisco Javier [0000-0002-6320-5391] González-Pérez, José Antonio [0000-0001-7607-1444] Campos, Alexandre Oliveira [0000-0003-4621-885X] Cameán Fernández, A. M. [0000-0003-1524-748X] Vasconcelos, Vítor [0000-0003-3585-2417] Póster presentado en el 1st Iberian Meeting in Separation Sciences & Mass Spectrometry, XIX Conference of the Spanish Society of Chromatography and Related Techniques (SECyTA), IX Conference of the Spanish Society of Mass Spectrometry (SEEM), VI Conference of the Mass Spectrometry Group of the Portuguese Society of Chemistry (SPQ). Santiago de Compostela, October 8th-11th (2019). Cylindrospermopsin (CYN) and Microcystin‐LR (MC‐LR) are biotoxins produced by cyanobacteria species that, as consequence of human‐induced environmental changes i.e. increase of temperature due to climate change and the eutrophication of waters, proliferate together with an increase in the presence of the associated cyanotoxins. Both toxins are usually found in the aquatic environment, filter feeding organisms such as mussels are exposed continuously an at such quantity that may be the cause of metabolic alterations. Analytical pyrolysis (Py‐GC/MS) was used to evaluate compositional changes in M. galloprovincialis after exposure for 14 days to cyanotoxins of Chrysosporum ovalisporum (CYN) (0.785 μg mL‐1), Microcystis aeruginosa (MC‐LR) (2.3 x 10‐3 μg mL‐1) and a combination of both toxins (CYN + MC‐LR). A group of mussels not exposed to the toxins was also included as control. Pyrolysis of lyophilized mussel flesh produced complex chromatograms (100 different compounds) but very similar between treatments. Major groups found were N compounds (pyridine, N‐alkyl molecules) (18% ± 1.5) and peptide/protein derived (18% ± 2.4) that include alkyl indols and diketopiperazines (DKPs) as pyrolysis product from amino acid condensation, series of medium chain length (C14‐C22) saturated, mono and polyunsaturated fatty acids (45% ± 5.0), sterols (8% ± 0.6) and aromatics with undetermined origin (9% ± 1.01). Chemometric treatment of the chromatographic data (variable reduction, PCA and hierarchical cluster analyses) allow us to discriminate between the different mussel populations exposed to cyanotoxins. The results are discussed in terms of the possible effects exerted by the biotoxins in mussel composition and possible metabolic pathways affected. Ministerio de Ciencia Innovación y Universidades Projects (AGL2015‐64558‐R, CGL2016‐78937‐R MINECO / FEDER, UE). L. Diez‐Quijada thanks the MICIU for funding FPI research grant (BES‐2016‐078773). Desiré Monis is acknowledged for technical assistance. Peer reviewed 2019-10-17T10:00:39Z 2019-10-17T10:00:39Z 2019-10 póster de congreso http://purl.org/coar/resource_type/c_6670 1st Iberian Meeting in Separation Sciences & Mass Spectrometry. Póster 108 (2019) http://hdl.handle.net/10261/192806 http://dx.doi.org/10.13039/501100003329 http://dx.doi.org/10.13039/501100000780 en #PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2015-64558-R info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/CGL2016-78937-R Sí open Sociedad Española de Cromatografía y Técnicas Afines |
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Póster presentado en el 1st Iberian Meeting in Separation Sciences & Mass Spectrometry, XIX Conference of the Spanish Society of Chromatography and Related Techniques (SECyTA), IX Conference of the Spanish Society of Mass Spectrometry (SEEM), VI Conference of the Mass Spectrometry Group of the Portuguese Society of Chemistry (SPQ). Santiago de Compostela, October 8th-11th (2019). |
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Ministerio de Economía y Competitividad (España) |
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Ministerio de Economía y Competitividad (España) Diez‐Quijada, Leticia de Oliveira, Flavio L. Jos, A. Cameán Fernández, A. M. Aparicio-Ruiz, R. Vasconcelos, Vítor Campos, Alexandre Oliveira González-Vila, Francisco Javier González-Pérez, José Antonio |
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póster de congreso |
author |
Diez‐Quijada, Leticia de Oliveira, Flavio L. Jos, A. Cameán Fernández, A. M. Aparicio-Ruiz, R. Vasconcelos, Vítor Campos, Alexandre Oliveira González-Vila, Francisco Javier González-Pérez, José Antonio |
spellingShingle |
Diez‐Quijada, Leticia de Oliveira, Flavio L. Jos, A. Cameán Fernández, A. M. Aparicio-Ruiz, R. Vasconcelos, Vítor Campos, Alexandre Oliveira González-Vila, Francisco Javier González-Pérez, José Antonio Alterations in Mediterranean mussel (Mytilus galloprovincialis) composition exposed to cyanotoxins as revealed by nalytical pyrolysis |
author_sort |
Diez‐Quijada, Leticia |
title |
Alterations in Mediterranean mussel (Mytilus galloprovincialis) composition exposed to cyanotoxins as revealed by nalytical pyrolysis |
title_short |
Alterations in Mediterranean mussel (Mytilus galloprovincialis) composition exposed to cyanotoxins as revealed by nalytical pyrolysis |
title_full |
Alterations in Mediterranean mussel (Mytilus galloprovincialis) composition exposed to cyanotoxins as revealed by nalytical pyrolysis |
title_fullStr |
Alterations in Mediterranean mussel (Mytilus galloprovincialis) composition exposed to cyanotoxins as revealed by nalytical pyrolysis |
title_full_unstemmed |
Alterations in Mediterranean mussel (Mytilus galloprovincialis) composition exposed to cyanotoxins as revealed by nalytical pyrolysis |
title_sort |
alterations in mediterranean mussel (mytilus galloprovincialis) composition exposed to cyanotoxins as revealed by nalytical pyrolysis |
publisher |
Sociedad Española de Cromatografía y Técnicas Afines |
publishDate |
2019-10 |
url |
http://hdl.handle.net/10261/192806 http://dx.doi.org/10.13039/501100003329 http://dx.doi.org/10.13039/501100000780 |
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