Fluorescent measurements of dna, rna and proteins to perform comparative analyses of microbial communities from the environments
Simple and rapid methods for the quantification of DNA, RNA and proteins using specific fluorescent dyes are proposed for the comparison and monitoring of microbial communities from the environment. The purpose of this study was the use of straightforward in situ methods which voided the need for preservation of samples and the risk of potential degradation and quantitative changes during transportation. Aside from this, methods used to obtain information on environmental microbial communities are generally time-consuming and present certain difficulty above all when working on solid substrates such as soils and rocks. New generation fluorescent dyes that bind specifically to DNA, RNA and proteins allow simple and rapid estimates of these biomolecules in crude environmental samples.
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Format: | artículo biblioteca |
Language: | English |
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John Wiley & Sons
2009-09-02
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Subjects: | Fluorescent Measurements, DNA, RNA, Proteins, |
Online Access: | http://hdl.handle.net/10261/17633 |
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dig-irnas-es-10261-176332019-02-27T09:20:26Z Fluorescent measurements of dna, rna and proteins to perform comparative analyses of microbial communities from the environments Portillo Guisado, María del Carmen González Grau, Juan Miguel Ministerio de Educación y Ciencia (España) Fluorescent Measurements DNA RNA Proteins Simple and rapid methods for the quantification of DNA, RNA and proteins using specific fluorescent dyes are proposed for the comparison and monitoring of microbial communities from the environment. The purpose of this study was the use of straightforward in situ methods which voided the need for preservation of samples and the risk of potential degradation and quantitative changes during transportation. Aside from this, methods used to obtain information on environmental microbial communities are generally time-consuming and present certain difficulty above all when working on solid substrates such as soils and rocks. New generation fluorescent dyes that bind specifically to DNA, RNA and proteins allow simple and rapid estimates of these biomolecules in crude environmental samples. The authors acknowledge support through project CGL2006-11561/BTE from the Spanish Ministry of Education and Science. Peer reviewed 2009-10-13T14:09:56Z 2009-10-13T14:09:56Z 2009-09-02 artículo http://purl.org/coar/resource_type/c_6501 Journal of Rapid Methods and Automation in Microbiology 17(3): 398-410 (2009) 1060-3999 http://hdl.handle.net/10261/17633 10.1111/j.1745-4581.2009.00179.x 1745-4581 en http://dx.doi.org/10.1111/j.1745-4581.2009.00179.x none 579917 bytes image/jpeg John Wiley & Sons |
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Fluorescent Measurements DNA RNA Proteins Fluorescent Measurements DNA RNA Proteins |
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Fluorescent Measurements DNA RNA Proteins Fluorescent Measurements DNA RNA Proteins Portillo Guisado, María del Carmen González Grau, Juan Miguel Fluorescent measurements of dna, rna and proteins to perform comparative analyses of microbial communities from the environments |
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Simple and rapid methods for the quantification of DNA, RNA and proteins using specific fluorescent dyes are proposed for the comparison and monitoring of microbial communities from the environment. The purpose of this study was the use of straightforward in situ methods which voided the need for preservation of samples and the risk of potential degradation and quantitative changes during transportation. Aside from this, methods used to obtain information on environmental microbial communities are generally time-consuming and present certain difficulty above all when working on solid substrates such as soils and rocks. New generation fluorescent dyes that bind specifically to DNA, RNA and proteins allow simple and rapid estimates of these biomolecules in crude environmental samples. |
author2 |
Ministerio de Educación y Ciencia (España) |
author_facet |
Ministerio de Educación y Ciencia (España) Portillo Guisado, María del Carmen González Grau, Juan Miguel |
format |
artículo |
topic_facet |
Fluorescent Measurements DNA RNA Proteins |
author |
Portillo Guisado, María del Carmen González Grau, Juan Miguel |
author_sort |
Portillo Guisado, María del Carmen |
title |
Fluorescent measurements of dna, rna and proteins to perform comparative analyses of microbial communities from the environments |
title_short |
Fluorescent measurements of dna, rna and proteins to perform comparative analyses of microbial communities from the environments |
title_full |
Fluorescent measurements of dna, rna and proteins to perform comparative analyses of microbial communities from the environments |
title_fullStr |
Fluorescent measurements of dna, rna and proteins to perform comparative analyses of microbial communities from the environments |
title_full_unstemmed |
Fluorescent measurements of dna, rna and proteins to perform comparative analyses of microbial communities from the environments |
title_sort |
fluorescent measurements of dna, rna and proteins to perform comparative analyses of microbial communities from the environments |
publisher |
John Wiley & Sons |
publishDate |
2009-09-02 |
url |
http://hdl.handle.net/10261/17633 |
work_keys_str_mv |
AT portilloguisadomariadelcarmen fluorescentmeasurementsofdnarnaandproteinstoperformcomparativeanalysesofmicrobialcommunitiesfromtheenvironments AT gonzalezgraujuanmiguel fluorescentmeasurementsofdnarnaandproteinstoperformcomparativeanalysesofmicrobialcommunitiesfromtheenvironments |
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1777664149077098496 |