Determination of propyl propane thiosulfonate in plasma and tissues by UHPLC-MS/MS orbitrap and analytical pyrolysis (Py-GC-MS)
Abstracts of the 52nd Congress of the European Societies of Toxicology (EUROTOX) Fibes Congress Center Seville, Spain, 04th-07th September 2016
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| Language: | English |
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Elsevier
2016-09
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| Online Access: | http://hdl.handle.net/10261/153240 http://dx.doi.org/10.13039/501100004837 http://dx.doi.org/10.13039/501100003329 http://dx.doi.org/10.13039/501100011011 |
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dig-irnas-es-10261-1532402018-09-11T11:35:14Z Determination of propyl propane thiosulfonate in plasma and tissues by UHPLC-MS/MS orbitrap and analytical pyrolysis (Py-GC-MS) Cameán Fernández, A. M. Mellado-García, Pilar Puerto Rodríguez, María Prieto Ortega, A. I. Pichardo, S. Jiménez Morillo, N. T. González-Pérez, José Antonio Ministerio de Ciencia e Innovación (España) Ministerio de Economía y Competitividad (España) Junta de Andalucía Abstracts of the 52nd Congress of the European Societies of Toxicology (EUROTOX) Fibes Congress Center Seville, Spain, 04th-07th September 2016 Garlic and onion contain biologically active substances, including volatile organosulfur compounds (OSCs), which may be responsible for their antimicrobial and antioxidant properties. The isolation of the compound propyl propane thiosulfonate (PTSO) affords a stable, active agent to be used in the food industry. PTSO may be incorporated into an inert commercial alimentary support to produce active packaging. According to the recommendations of the OECD 489 and 474, several methods are necessary to verifying that the compound could reach to the target tissue or general circulation. In this sense, the aim of this study was determinate the presence of PTSO in plasma and tissues (stomach and liver) of rats following oral administration (55 mg/kg bw) for later assessed the genotoxicity. The quantification of PTSO in plasma was performed by UHPLC–MS/MS Orbitrap and its identification and degradation products in tissues (stomach and liver) was performed by analytical pyrolysis (Py-GC–MS). PTSO was not detected at any concentration tested in plasma, and in the stomach six OSCs compounds of PTSO were detected. Among these, dimethyl disulphide and methyl propyldisulphide are likely to have originated from PTSO, which seems to suffer hydrolysis reactions. Signs of the presence of PTSO were also found in liver of treated rats by detecting a single OSC: 2-methyl-2-(methylthio) propane, indicating the influence of distribution and metabolism processes. These results indicate that Py-GC-MS is an adequate technique that provides first evidences of the absorption of PTSO and its presence including metabolites in tissues. Projects AGL2012-38357-C02-01, CGL2012-38655-C04-01, and AGR-7252. No 2017-07-17T10:52:54Z 2017-07-17T10:52:54Z 2016-09 comunicación de congreso http://purl.org/coar/resource_type/c_5794 Toxicology Letters 258 ( Supplement): S167-S167 (2016) 1879-3169 http://hdl.handle.net/10261/153240 10.1016/j.toxlet.2016.06.1628 http://dx.doi.org/10.13039/501100004837 http://dx.doi.org/10.13039/501100003329 http://dx.doi.org/10.13039/501100011011 en Publisher's version https://doi.org/10.1016/j.toxlet.2016.06.1628 Sí none Elsevier |
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| description |
Abstracts of the 52nd Congress of the European Societies of Toxicology (EUROTOX) Fibes Congress Center Seville, Spain, 04th-07th September 2016 |
| author2 |
Ministerio de Ciencia e Innovación (España) |
| author_facet |
Ministerio de Ciencia e Innovación (España) Cameán Fernández, A. M. Mellado-García, Pilar Puerto Rodríguez, María Prieto Ortega, A. I. Pichardo, S. Jiménez Morillo, N. T. González-Pérez, José Antonio |
| format |
comunicación de congreso |
| author |
Cameán Fernández, A. M. Mellado-García, Pilar Puerto Rodríguez, María Prieto Ortega, A. I. Pichardo, S. Jiménez Morillo, N. T. González-Pérez, José Antonio |
| spellingShingle |
Cameán Fernández, A. M. Mellado-García, Pilar Puerto Rodríguez, María Prieto Ortega, A. I. Pichardo, S. Jiménez Morillo, N. T. González-Pérez, José Antonio Determination of propyl propane thiosulfonate in plasma and tissues by UHPLC-MS/MS orbitrap and analytical pyrolysis (Py-GC-MS) |
| author_sort |
Cameán Fernández, A. M. |
| title |
Determination of propyl propane thiosulfonate in plasma and tissues by UHPLC-MS/MS orbitrap and analytical pyrolysis (Py-GC-MS) |
| title_short |
Determination of propyl propane thiosulfonate in plasma and tissues by UHPLC-MS/MS orbitrap and analytical pyrolysis (Py-GC-MS) |
| title_full |
Determination of propyl propane thiosulfonate in plasma and tissues by UHPLC-MS/MS orbitrap and analytical pyrolysis (Py-GC-MS) |
| title_fullStr |
Determination of propyl propane thiosulfonate in plasma and tissues by UHPLC-MS/MS orbitrap and analytical pyrolysis (Py-GC-MS) |
| title_full_unstemmed |
Determination of propyl propane thiosulfonate in plasma and tissues by UHPLC-MS/MS orbitrap and analytical pyrolysis (Py-GC-MS) |
| title_sort |
determination of propyl propane thiosulfonate in plasma and tissues by uhplc-ms/ms orbitrap and analytical pyrolysis (py-gc-ms) |
| publisher |
Elsevier |
| publishDate |
2016-09 |
| url |
http://hdl.handle.net/10261/153240 http://dx.doi.org/10.13039/501100004837 http://dx.doi.org/10.13039/501100003329 http://dx.doi.org/10.13039/501100011011 |
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