Hydrolytic enzyme activity enhanced by Barium supplementation
10 páginas.-- 2 figuras.-- 1 tabla.-- 26 referencias
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Format: | artículo biblioteca |
Language: | English |
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AIMS Press
2016-10-21
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Subjects: | Barium, Extracellular enzyme activity, Hydrolytic activity, Cellulase, Esterase, |
Online Access: | http://hdl.handle.net/10261/139247 http://dx.doi.org/10.13039/501100003329 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100011011 |
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dig-irnas-es-10261-1392472019-05-13T08:48:21Z Hydrolytic enzyme activity enhanced by Barium supplementation Muñoz, Camilo Fermoso, Fernando G. Rivas, Mariela González Grau, Juan Miguel Ministerio de Economía y Competitividad (España) Junta de Andalucía European Commission Barium Extracellular enzyme activity Hydrolytic activity Cellulase Esterase 10 páginas.-- 2 figuras.-- 1 tabla.-- 26 referencias Hydrolysis of polymers is a first and often limiting step during the degradation of plant residues. Plant biomass is generally a major component of waste residues and a major renewable resource to obtain a variety of secondary products including biofuels. Improving the performance of enzymatic hydrolysis of plant material with minimum costs and limiting the use of additional microbial biomass or hydrolytic enzymes directly influences competitiveness of these green biotechnological processes. In this study, we cloned and expressed a cellulase and two esterases recovered from environmental thermophilic soil bacterial communities and characterize their optimum activity conditions including the effect of several metal ions. Results showed that supplementing these hydrolytic reactions with Barium increases the activity of these extracellular hydrolytic enzymes. This observation represents a simple but major improvement to enhance the efficiency and competitiveness of this process within an increasingly important biotechnological sector. Support from the Spanish Ministry of Economy and Competitiveness (CONSOLIDER CSD2009-00006, CGL2014-58762-P, CTM2014-55095), the Andalusian Government (BIO288 and RNM2529), both cofinanced by FEDER funds, intramural project OEP2011 (201570I020), the mobility programme 003-ABEL-CM-2013 (NILS Science and Sustainability programme, EEA grants), the mobility and coordination European project ALGAENET (Marie Curie Actions IRSES- 295165, FP7-PEOPLE-2011) and COST Action ES1302. Peer reviewed 2016-10-24T11:15:59Z 2016-10-24T11:15:59Z 2016-10-21 artículo http://purl.org/coar/resource_type/c_6501 AIMS Microbiology 2(4): 402-411 (2016) http://hdl.handle.net/10261/139247 10.3934/microbiol.2016.4.402 2471-1888 http://dx.doi.org/10.13039/501100003329 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100011011 en #PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/CGL2014-58762-P info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/CTM2014-55095-R Publisher's version http://dx.doi.org/10.1002/10.3934/microbiol.2016.4.402 Sí open AIMS Press |
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Barium Extracellular enzyme activity Hydrolytic activity Cellulase Esterase Barium Extracellular enzyme activity Hydrolytic activity Cellulase Esterase |
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Barium Extracellular enzyme activity Hydrolytic activity Cellulase Esterase Barium Extracellular enzyme activity Hydrolytic activity Cellulase Esterase Muñoz, Camilo Fermoso, Fernando G. Rivas, Mariela González Grau, Juan Miguel Hydrolytic enzyme activity enhanced by Barium supplementation |
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10 páginas.-- 2 figuras.-- 1 tabla.-- 26 referencias |
author2 |
Ministerio de Economía y Competitividad (España) |
author_facet |
Ministerio de Economía y Competitividad (España) Muñoz, Camilo Fermoso, Fernando G. Rivas, Mariela González Grau, Juan Miguel |
format |
artículo |
topic_facet |
Barium Extracellular enzyme activity Hydrolytic activity Cellulase Esterase |
author |
Muñoz, Camilo Fermoso, Fernando G. Rivas, Mariela González Grau, Juan Miguel |
author_sort |
Muñoz, Camilo |
title |
Hydrolytic enzyme activity enhanced by Barium supplementation |
title_short |
Hydrolytic enzyme activity enhanced by Barium supplementation |
title_full |
Hydrolytic enzyme activity enhanced by Barium supplementation |
title_fullStr |
Hydrolytic enzyme activity enhanced by Barium supplementation |
title_full_unstemmed |
Hydrolytic enzyme activity enhanced by Barium supplementation |
title_sort |
hydrolytic enzyme activity enhanced by barium supplementation |
publisher |
AIMS Press |
publishDate |
2016-10-21 |
url |
http://hdl.handle.net/10261/139247 http://dx.doi.org/10.13039/501100003329 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100011011 |
work_keys_str_mv |
AT munozcamilo hydrolyticenzymeactivityenhancedbybariumsupplementation AT fermosofernandog hydrolyticenzymeactivityenhancedbybariumsupplementation AT rivasmariela hydrolyticenzymeactivityenhancedbybariumsupplementation AT gonzalezgraujuanmiguel hydrolyticenzymeactivityenhancedbybariumsupplementation |
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1777664888834883584 |