Analysis of a modern hybrid and an ancient sugarcane implicates a complex interplay of factors in affecting recalcitrance to cellulosic ethanol production
24 páginas.-- 7 figuras.-- 4 tablas.-- 80 referencias.-- Supporting Information 1S con 1 tabla.
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2015-08-07
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dig-irnas-es-10261-1228192022-08-17T11:05:15Z Analysis of a modern hybrid and an ancient sugarcane implicates a complex interplay of factors in affecting recalcitrance to cellulosic ethanol production de Carli Poelking, V. G. Giordano, Andrea Ricci-Silva, M. E. Williams, T. C. R. Peçanha, Diego A. Ventrella, M. C. Rencoret, Jorge Ralph, John Barbosa, M. H. P. Loureiro, Marcelo Universidad Federal de Viçosa 24 páginas.-- 7 figuras.-- 4 tablas.-- 80 referencias.-- Supporting Information 1S con 1 tabla. Abundant evidence exists to support a role for lignin as an important element in biomass recalcitrance. However, several independent studies have also shown that factors apart from lignin are also relevant and overall, the relative importance of different recalcitrance traits remains in dispute. In this study we used two genetically distant sugarcane genotypes, and performed a correlational study with the variation in anatomical parameters, cell wall composition, and recalcitrance factors between these genotypes. In addition we also tracked alterations in these characteristics in internodes at different stages of development. Significant differences in the development of the culm between the genotypes were associated with clear differential distributions of lignin content and composition that were not correlated with saccharification and fermentation yield. Given the strong influence of the environment on lignin content and composition, we hypothesized that sampling within a single plant could allow us to more easily interpret recalcitrance and changes in lignin biosynthesis than analysing variations between different genotypes with extensive changes in plant morphology and culm anatomy. The syringyl/guaiacyl (S/G) ratio was higher in the oldest internode of the modern genotype, but S/G ratio was not correlated with enzymatic hydrolysis yield nor fermentation efficiency. Curiously we observed a strong positive correlation between ferulate ester level and cellulose conversion efficiency. Together, these data support the hypothesis that biomass enzymatic hydrolysis recalcitrance is governed by a quantitative heritage rather than a single trait. Copyright: © 2015 Guzzo de Carli Poelking et al. The authors would like to thank the Nucleo de Analise de Biomoleculas (NuBioMol), Universidade Federal de Vicosa, MG, Brazil for the use of the UPLC-QQQ and Carlos Joulbert de Souza for assistance with saccharification and fermentation assays. Peer reviewed 2015-09-30T07:50:46Z 2015-09-30T07:50:46Z 2015-08-07 artículo http://purl.org/coar/resource_type/c_6501 PLoS ONE 10(8): e0134964 (2015) 1932-6203 http://hdl.handle.net/10261/122819 10.1371/journal.pone.0134964 26252208 en Publisher's version http://dx.doi.org/10.1371/journal.pone.0134964 Sí open Public Library of Science |
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24 páginas.-- 7 figuras.-- 4 tablas.-- 80 referencias.-- Supporting Information 1S con 1 tabla. |
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Universidad Federal de Viçosa |
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Universidad Federal de Viçosa de Carli Poelking, V. G. Giordano, Andrea Ricci-Silva, M. E. Williams, T. C. R. Peçanha, Diego A. Ventrella, M. C. Rencoret, Jorge Ralph, John Barbosa, M. H. P. Loureiro, Marcelo |
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de Carli Poelking, V. G. Giordano, Andrea Ricci-Silva, M. E. Williams, T. C. R. Peçanha, Diego A. Ventrella, M. C. Rencoret, Jorge Ralph, John Barbosa, M. H. P. Loureiro, Marcelo |
| spellingShingle |
de Carli Poelking, V. G. Giordano, Andrea Ricci-Silva, M. E. Williams, T. C. R. Peçanha, Diego A. Ventrella, M. C. Rencoret, Jorge Ralph, John Barbosa, M. H. P. Loureiro, Marcelo Analysis of a modern hybrid and an ancient sugarcane implicates a complex interplay of factors in affecting recalcitrance to cellulosic ethanol production |
| author_sort |
de Carli Poelking, V. G. |
| title |
Analysis of a modern hybrid and an ancient sugarcane implicates a complex interplay of factors in affecting recalcitrance to cellulosic ethanol production |
| title_short |
Analysis of a modern hybrid and an ancient sugarcane implicates a complex interplay of factors in affecting recalcitrance to cellulosic ethanol production |
| title_full |
Analysis of a modern hybrid and an ancient sugarcane implicates a complex interplay of factors in affecting recalcitrance to cellulosic ethanol production |
| title_fullStr |
Analysis of a modern hybrid and an ancient sugarcane implicates a complex interplay of factors in affecting recalcitrance to cellulosic ethanol production |
| title_full_unstemmed |
Analysis of a modern hybrid and an ancient sugarcane implicates a complex interplay of factors in affecting recalcitrance to cellulosic ethanol production |
| title_sort |
analysis of a modern hybrid and an ancient sugarcane implicates a complex interplay of factors in affecting recalcitrance to cellulosic ethanol production |
| publisher |
Public Library of Science |
| publishDate |
2015-08-07 |
| url |
http://hdl.handle.net/10261/122819 |
| work_keys_str_mv |
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