Isolation of potentially pathogenic strains of Acanthamoeba in wild squirrels from the Canary Islands and Morocco
In this study, a survey was conducted in order to determine the presence and pathogenic potential of free-living amoebae of Acanthamoeba genus in a population of Barbary Ground squirrels in Fuerteventura Island (Canary Islands, Spain) and Morocco. Identification of Acanthamoeba was based on the morphology of cyst and trophozoite forms and PCR amplification with a genus specific primer pair. The pathogenic potential of Acanthamoeba isolates was characterized using PCRs with two primer pairs related to Acanthamoeba pathogenesis and cytotoxicity assays using human corneal cells. Isolates genotypes were also determined after ribosomal DNA sequencing. These data revealed that the potentially pathogenic isolates belonged to T3 and T4. To our knowledge, this is the first report presenting the isolation of potentially pathogenic Acanthamoeba strains in wild squirrels, and it is also the first description of the pathogenic T3 and T4 Acanthamoeba genotypes in Fuerteventura and Morocco.
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2007
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| Subjects: | Acanthamoeba | Barbary ground squirrel | Canary Islands | Environmental health | Morocco | Pathogen | Public health, http://metadata.un.org/sdg/15, Protect, restore and promote sustainable use of terrestrial ecosystems, sustainably manage forests, combat desertification, and halt and reverse land degradation and halt biodiversity loss, |
| Online Access: | http://hdl.handle.net/10261/303304 http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000249201800010&KeyUID=WOS:000249201800010 |
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dig-ipna-es-10261-3033042024-05-17T20:37:54Z Isolation of potentially pathogenic strains of Acanthamoeba in wild squirrels from the Canary Islands and Morocco Lorenzo-Morales, Jacob López-Darias, Marta Martinez-Carretero, Enrique Valladares, Basilio Acanthamoeba | Barbary ground squirrel | Canary Islands | Environmental health | Morocco | Pathogen | Public health http://metadata.un.org/sdg/15 Protect, restore and promote sustainable use of terrestrial ecosystems, sustainably manage forests, combat desertification, and halt and reverse land degradation and halt biodiversity loss In this study, a survey was conducted in order to determine the presence and pathogenic potential of free-living amoebae of Acanthamoeba genus in a population of Barbary Ground squirrels in Fuerteventura Island (Canary Islands, Spain) and Morocco. Identification of Acanthamoeba was based on the morphology of cyst and trophozoite forms and PCR amplification with a genus specific primer pair. The pathogenic potential of Acanthamoeba isolates was characterized using PCRs with two primer pairs related to Acanthamoeba pathogenesis and cytotoxicity assays using human corneal cells. Isolates genotypes were also determined after ribosomal DNA sequencing. These data revealed that the potentially pathogenic isolates belonged to T3 and T4. To our knowledge, this is the first report presenting the isolation of potentially pathogenic Acanthamoeba strains in wild squirrels, and it is also the first description of the pathogenic T3 and T4 Acanthamoeba genotypes in Fuerteventura and Morocco. Peer reviewed 2023-03-15T21:05:22Z 2023-03-15T21:05:22Z 2007 artículo http://purl.org/coar/resource_type/c_6501 0014-4894 http://hdl.handle.net/10261/303304 10.1016/j.exppara.2007.03.014 17459380 2-s2.0-34547105105 000249201800010 http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000249201800010&KeyUID=WOS:000249201800010 Experimental Parasitology Sí open |
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Acanthamoeba | Barbary ground squirrel | Canary Islands | Environmental health | Morocco | Pathogen | Public health http://metadata.un.org/sdg/15 Protect, restore and promote sustainable use of terrestrial ecosystems, sustainably manage forests, combat desertification, and halt and reverse land degradation and halt biodiversity loss Acanthamoeba | Barbary ground squirrel | Canary Islands | Environmental health | Morocco | Pathogen | Public health http://metadata.un.org/sdg/15 Protect, restore and promote sustainable use of terrestrial ecosystems, sustainably manage forests, combat desertification, and halt and reverse land degradation and halt biodiversity loss |
| spellingShingle |
Acanthamoeba | Barbary ground squirrel | Canary Islands | Environmental health | Morocco | Pathogen | Public health http://metadata.un.org/sdg/15 Protect, restore and promote sustainable use of terrestrial ecosystems, sustainably manage forests, combat desertification, and halt and reverse land degradation and halt biodiversity loss Acanthamoeba | Barbary ground squirrel | Canary Islands | Environmental health | Morocco | Pathogen | Public health http://metadata.un.org/sdg/15 Protect, restore and promote sustainable use of terrestrial ecosystems, sustainably manage forests, combat desertification, and halt and reverse land degradation and halt biodiversity loss Lorenzo-Morales, Jacob López-Darias, Marta Martinez-Carretero, Enrique Valladares, Basilio Isolation of potentially pathogenic strains of Acanthamoeba in wild squirrels from the Canary Islands and Morocco |
| description |
In this study, a survey was conducted in order to determine the presence and pathogenic potential of free-living amoebae of Acanthamoeba genus in a population of Barbary Ground squirrels in Fuerteventura Island (Canary Islands, Spain) and Morocco. Identification of Acanthamoeba was based on the morphology of cyst and trophozoite forms and PCR amplification with a genus specific primer pair. The pathogenic potential of Acanthamoeba isolates was characterized using PCRs with two primer pairs related to Acanthamoeba pathogenesis and cytotoxicity assays using human corneal cells. Isolates genotypes were also determined after ribosomal DNA sequencing. These data revealed that the potentially pathogenic isolates belonged to T3 and T4. To our knowledge, this is the first report presenting the isolation of potentially pathogenic Acanthamoeba strains in wild squirrels, and it is also the first description of the pathogenic T3 and T4 Acanthamoeba genotypes in Fuerteventura and Morocco. |
| format |
artículo |
| topic_facet |
Acanthamoeba | Barbary ground squirrel | Canary Islands | Environmental health | Morocco | Pathogen | Public health http://metadata.un.org/sdg/15 Protect, restore and promote sustainable use of terrestrial ecosystems, sustainably manage forests, combat desertification, and halt and reverse land degradation and halt biodiversity loss |
| author |
Lorenzo-Morales, Jacob López-Darias, Marta Martinez-Carretero, Enrique Valladares, Basilio |
| author_facet |
Lorenzo-Morales, Jacob López-Darias, Marta Martinez-Carretero, Enrique Valladares, Basilio |
| author_sort |
Lorenzo-Morales, Jacob |
| title |
Isolation of potentially pathogenic strains of Acanthamoeba in wild squirrels from the Canary Islands and Morocco |
| title_short |
Isolation of potentially pathogenic strains of Acanthamoeba in wild squirrels from the Canary Islands and Morocco |
| title_full |
Isolation of potentially pathogenic strains of Acanthamoeba in wild squirrels from the Canary Islands and Morocco |
| title_fullStr |
Isolation of potentially pathogenic strains of Acanthamoeba in wild squirrels from the Canary Islands and Morocco |
| title_full_unstemmed |
Isolation of potentially pathogenic strains of Acanthamoeba in wild squirrels from the Canary Islands and Morocco |
| title_sort |
isolation of potentially pathogenic strains of acanthamoeba in wild squirrels from the canary islands and morocco |
| publishDate |
2007 |
| url |
http://hdl.handle.net/10261/303304 http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=ORCID&SrcApp=OrcidOrg&DestLinkType=FullRecord&DestApp=WOS_CPL&KeyUT=WOS:000249201800010&KeyUID=WOS:000249201800010 |
| work_keys_str_mv |
AT lorenzomoralesjacob isolationofpotentiallypathogenicstrainsofacanthamoebainwildsquirrelsfromthecanaryislandsandmorocco AT lopezdariasmarta isolationofpotentiallypathogenicstrainsofacanthamoebainwildsquirrelsfromthecanaryislandsandmorocco AT martinezcarreteroenrique isolationofpotentiallypathogenicstrainsofacanthamoebainwildsquirrelsfromthecanaryislandsandmorocco AT valladaresbasilio isolationofpotentiallypathogenicstrainsofacanthamoebainwildsquirrelsfromthecanaryislandsandmorocco |
| _version_ |
1802820483580690432 |