Isolation, antioxidant and antimicrobial activities of ecdysteroids from serratula cichoracea

Isolation, antioxidant and antimicrobial activities of ecdysteroids from serratula cichoracea

[Background] Several Serratula species are used in folk medicine and showed interesting biological activities. The objective of this work was to continue the investigation of the chemical composition of the ethyl acetate extract of the flowers of Serratula cichoracea which in our previous study showed a significant antioxidant activity in 1,1-diphenyl-2-picrylhydrazyl radical (DPPH•) essay compared to quercetin used as control molecule and to evaluate the isolated compounds for their antioxidant and antimicrobial activities, [Methods] Ethyl acetate extract (15 g) was fractionated by column chromatography on silica gel. After purification on preparative plates, the isolated compounds were identified by spectral analyses mainly high resolution electrospray ionisation mass spectrometry (HR-ESIMS) and one-and two-dimensionnal nuclear magnetic resonance spectroscopy experiments, and were evaluated in vitro for antioxidant activity using 1,1-diphenyl-2-picrylhydrazyl radical and for antimicrobial properties, [Results] A new phytoecdysteroid named 22-epi-ajugasterone C (1) together with ajugasterone C (2), were isolated. Compounds 1 and 2 had weak scavenging effect compared with myricetin used as positive control, whereas the two compounds showed antimicrobial properties against multiresistant strains like Staphylococcus aureus, Escherichia coli, Serratia sp., Klebsiella pneumoniae and Candida albicans, [Conclusion] The new isolated phytoecdysteroid and its epimer ajugasterone C, responded to their effectiveness against different various antibiotic-resistant bacteria.

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Main Authors: Aliouche, Lamia, Larguet, Habiba, Amrani, Amel, León, Francisco, Brouard, Ignacio, Benayache, Samir, Zama, Djamila, Meraihi, Zahia, Benayache, Fadila
Format: artículo biblioteca
Published: Bentham Science Publishers 2018
Online Access:http://hdl.handle.net/10261/180434
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spelling dig-ipna-es-10261-1804342020-12-13T09:24:46Z Isolation, antioxidant and antimicrobial activities of ecdysteroids from serratula cichoracea Aliouche, Lamia Larguet, Habiba Amrani, Amel León, Francisco Brouard, Ignacio Benayache, Samir Zama, Djamila Meraihi, Zahia Benayache, Fadila [Background] Several Serratula species are used in folk medicine and showed interesting biological activities. The objective of this work was to continue the investigation of the chemical composition of the ethyl acetate extract of the flowers of Serratula cichoracea which in our previous study showed a significant antioxidant activity in 1,1-diphenyl-2-picrylhydrazyl radical (DPPH•) essay compared to quercetin used as control molecule and to evaluate the isolated compounds for their antioxidant and antimicrobial activities, [Methods] Ethyl acetate extract (15 g) was fractionated by column chromatography on silica gel. After purification on preparative plates, the isolated compounds were identified by spectral analyses mainly high resolution electrospray ionisation mass spectrometry (HR-ESIMS) and one-and two-dimensionnal nuclear magnetic resonance spectroscopy experiments, and were evaluated in vitro for antioxidant activity using 1,1-diphenyl-2-picrylhydrazyl radical and for antimicrobial properties, [Results] A new phytoecdysteroid named 22-epi-ajugasterone C (1) together with ajugasterone C (2), were isolated. Compounds 1 and 2 had weak scavenging effect compared with myricetin used as positive control, whereas the two compounds showed antimicrobial properties against multiresistant strains like Staphylococcus aureus, Escherichia coli, Serratia sp., Klebsiella pneumoniae and Candida albicans, [Conclusion] The new isolated phytoecdysteroid and its epimer ajugasterone C, responded to their effectiveness against different various antibiotic-resistant bacteria. The authors are thankful to Dr. Djamel Sarri for the identification of Serratula cichoracea, and to DGRSDT and MESRS for financial support. Peer Reviewed 2019-04-24T07:20:11Z 2019-04-24T07:20:11Z 2018 2019-04-24T07:20:11Z artículo http://purl.org/coar/resource_type/c_6501 doi: 10.2174/1573407214666171211154922 e-issn: 1875-6646 issn: 1573-4072 Current Bioactive Compounds 14(1): 60-66 (2018) http://hdl.handle.net/10261/180434 10.2174/1573407214666171211154922 Postprint https://doi.org/10.2174/1573407214666171211154922 Sí none Bentham Science Publishers
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country España
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component Bibliográfico
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databasecode dig-ipna-es
tag biblioteca
region Europa del Sur
libraryname Biblioteca del IPNA España
description [Background] Several Serratula species are used in folk medicine and showed interesting biological activities. The objective of this work was to continue the investigation of the chemical composition of the ethyl acetate extract of the flowers of Serratula cichoracea which in our previous study showed a significant antioxidant activity in 1,1-diphenyl-2-picrylhydrazyl radical (DPPH•) essay compared to quercetin used as control molecule and to evaluate the isolated compounds for their antioxidant and antimicrobial activities, [Methods] Ethyl acetate extract (15 g) was fractionated by column chromatography on silica gel. After purification on preparative plates, the isolated compounds were identified by spectral analyses mainly high resolution electrospray ionisation mass spectrometry (HR-ESIMS) and one-and two-dimensionnal nuclear magnetic resonance spectroscopy experiments, and were evaluated in vitro for antioxidant activity using 1,1-diphenyl-2-picrylhydrazyl radical and for antimicrobial properties, [Results] A new phytoecdysteroid named 22-epi-ajugasterone C (1) together with ajugasterone C (2), were isolated. Compounds 1 and 2 had weak scavenging effect compared with myricetin used as positive control, whereas the two compounds showed antimicrobial properties against multiresistant strains like Staphylococcus aureus, Escherichia coli, Serratia sp., Klebsiella pneumoniae and Candida albicans, [Conclusion] The new isolated phytoecdysteroid and its epimer ajugasterone C, responded to their effectiveness against different various antibiotic-resistant bacteria.
format artículo
author Aliouche, Lamia
Larguet, Habiba
Amrani, Amel
León, Francisco
Brouard, Ignacio
Benayache, Samir
Zama, Djamila
Meraihi, Zahia
Benayache, Fadila
spellingShingle Aliouche, Lamia
Larguet, Habiba
Amrani, Amel
León, Francisco
Brouard, Ignacio
Benayache, Samir
Zama, Djamila
Meraihi, Zahia
Benayache, Fadila
Isolation, antioxidant and antimicrobial activities of ecdysteroids from serratula cichoracea
author_facet Aliouche, Lamia
Larguet, Habiba
Amrani, Amel
León, Francisco
Brouard, Ignacio
Benayache, Samir
Zama, Djamila
Meraihi, Zahia
Benayache, Fadila
author_sort Aliouche, Lamia
title Isolation, antioxidant and antimicrobial activities of ecdysteroids from serratula cichoracea
title_short Isolation, antioxidant and antimicrobial activities of ecdysteroids from serratula cichoracea
title_full Isolation, antioxidant and antimicrobial activities of ecdysteroids from serratula cichoracea
title_fullStr Isolation, antioxidant and antimicrobial activities of ecdysteroids from serratula cichoracea
title_full_unstemmed Isolation, antioxidant and antimicrobial activities of ecdysteroids from serratula cichoracea
title_sort isolation, antioxidant and antimicrobial activities of ecdysteroids from serratula cichoracea
publisher Bentham Science Publishers
publishDate 2018
url http://hdl.handle.net/10261/180434
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