Expression of TLR4 in swine as assessed by a newly developed monoclonal antibody
Toll-like receptors (TLRs) constitute an ancient family of pattern recognition receptors for conserved microbial structures that allow rapid detection of invading pathogens, triggering immune responses. TLR4 binds lipopolysaccharides (LPS) being involved in the recognition of Gram-negative bacteria. Herein we describe the generation and characterisation of a monoclonal antibody, named 3H3, against porcine TLR4. Its specificity was confirmed by reactivity with TLR4 expressing CHO cell transfectants. On peripheral blood leukocytes TLR4 was preferentially expressed on myelomonocytic cells, with monocytes expressing higher levels than granulocytes. Staining of lung tissue sections showed that TLR4 is also expressed on epithelial cells lining the bronchial tract, a distribution consistent with a surveillance function of bacterial invasion. © 2013 Elsevier B.V.
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | journal article biblioteca |
| Language: | eng |
| Published: |
2013
|
| Online Access: | http://hdl.handle.net/20.500.12792/975 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| id |
dig-inia-es-20.500.12792-975 |
|---|---|
| record_format |
koha |
| spelling |
dig-inia-es-20.500.12792-9752020-12-15T09:14:49Z Expression of TLR4 in swine as assessed by a newly developed monoclonal antibody Álvarez, B. Martínez de la Riva, P. Handler, A. Revilla, C. Alonso, F. Ezquerra, A. Domínguez, J. Toll-like receptors (TLRs) constitute an ancient family of pattern recognition receptors for conserved microbial structures that allow rapid detection of invading pathogens, triggering immune responses. TLR4 binds lipopolysaccharides (LPS) being involved in the recognition of Gram-negative bacteria. Herein we describe the generation and characterisation of a monoclonal antibody, named 3H3, against porcine TLR4. Its specificity was confirmed by reactivity with TLR4 expressing CHO cell transfectants. On peripheral blood leukocytes TLR4 was preferentially expressed on myelomonocytic cells, with monocytes expressing higher levels than granulocytes. Staining of lung tissue sections showed that TLR4 is also expressed on epithelial cells lining the bronchial tract, a distribution consistent with a surveillance function of bacterial invasion. © 2013 Elsevier B.V. 2020-10-22T11:42:11Z 2020-10-22T11:42:11Z 2013 journal article http://hdl.handle.net/20.500.12792/975 10.1016/j.vetimm.2012.12.020 eng Attribution-NonCommercial-ShareAlike 4.0 International http://creativecommons.org/licenses/by-nc-sa/4.0/ open access |
| institution |
INIA ES |
| collection |
DSpace |
| country |
España |
| countrycode |
ES |
| component |
Bibliográfico |
| access |
En linea |
| databasecode |
dig-inia-es |
| tag |
biblioteca |
| region |
Europa del Sur |
| libraryname |
Biblioteca del INIA España |
| language |
eng |
| description |
Toll-like receptors (TLRs) constitute an ancient family of pattern recognition receptors for conserved microbial structures that allow rapid detection of invading pathogens, triggering immune responses. TLR4 binds lipopolysaccharides (LPS) being involved in the recognition of Gram-negative bacteria. Herein we describe the generation and characterisation of a monoclonal antibody, named 3H3, against porcine TLR4. Its specificity was confirmed by reactivity with TLR4 expressing CHO cell transfectants. On peripheral blood leukocytes TLR4 was preferentially expressed on myelomonocytic cells, with monocytes expressing higher levels than granulocytes. Staining of lung tissue sections showed that TLR4 is also expressed on epithelial cells lining the bronchial tract, a distribution consistent with a surveillance function of bacterial invasion. © 2013 Elsevier B.V. |
| format |
journal article |
| author |
Álvarez, B. Martínez de la Riva, P. Handler, A. Revilla, C. Alonso, F. Ezquerra, A. Domínguez, J. |
| spellingShingle |
Álvarez, B. Martínez de la Riva, P. Handler, A. Revilla, C. Alonso, F. Ezquerra, A. Domínguez, J. Expression of TLR4 in swine as assessed by a newly developed monoclonal antibody |
| author_facet |
Álvarez, B. Martínez de la Riva, P. Handler, A. Revilla, C. Alonso, F. Ezquerra, A. Domínguez, J. |
| author_sort |
Álvarez, B. |
| title |
Expression of TLR4 in swine as assessed by a newly developed monoclonal antibody |
| title_short |
Expression of TLR4 in swine as assessed by a newly developed monoclonal antibody |
| title_full |
Expression of TLR4 in swine as assessed by a newly developed monoclonal antibody |
| title_fullStr |
Expression of TLR4 in swine as assessed by a newly developed monoclonal antibody |
| title_full_unstemmed |
Expression of TLR4 in swine as assessed by a newly developed monoclonal antibody |
| title_sort |
expression of tlr4 in swine as assessed by a newly developed monoclonal antibody |
| publishDate |
2013 |
| url |
http://hdl.handle.net/20.500.12792/975 |
| work_keys_str_mv |
AT alvarezb expressionoftlr4inswineasassessedbyanewlydevelopedmonoclonalantibody AT martinezdelarivap expressionoftlr4inswineasassessedbyanewlydevelopedmonoclonalantibody AT handlera expressionoftlr4inswineasassessedbyanewlydevelopedmonoclonalantibody AT revillac expressionoftlr4inswineasassessedbyanewlydevelopedmonoclonalantibody AT alonsof expressionoftlr4inswineasassessedbyanewlydevelopedmonoclonalantibody AT ezquerraa expressionoftlr4inswineasassessedbyanewlydevelopedmonoclonalantibody AT dominguezj expressionoftlr4inswineasassessedbyanewlydevelopedmonoclonalantibody |
| _version_ |
1758004517205442560 |