Pathological study of experimentally induced bovine respiratory syncytial viral infection in lambs
An experimental model was designed to characterize lesions in the lung of limbs inoculated with bovine respiratory syncytial virus (BRSV). Twenty-five Merino lambs of both sexes, with a live weight of 17 ± 3 kg, received an intratracheal inoculation of 20 ml saline solution containing 1.26 × 106 TCID50 BRSV (strain NMK-7) per ml. Lambs were slaughtered 1, 3, 7, 11 and 15 days postinoculation (DPI), and samples were taken for analysis using light-microscopic and immunohistochemical techniques. The results reflected the effect of the virus on airway epithelia. The presence of BRSV in ciliated bronchial and bronchiolar epithelial cells gave rise to cytopathological changes, including loss of cilia and cell necrosis; these changes might be expected to decrease the efficiency of mucociliary clearance, favouring the development of secondary bacterial bronchopneumonia. These results suggest a reduction in BRSV tropism for alveolar epithelia compared to bronchial and bronchiolar epithelia. Light-microscopic analysis revealed a narrowing of alveolar and airway lumina and a considerable interstitial inflammatory reaction.
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1996
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dig-inia-es-20.500.12792-52852020-12-15T09:55:02Z Pathological study of experimentally induced bovine respiratory syncytial viral infection in lambs Masot, A. J. Gomez-Tejedor, C. Gomez, L. Gazquez, A. Redondo, E. An experimental model was designed to characterize lesions in the lung of limbs inoculated with bovine respiratory syncytial virus (BRSV). Twenty-five Merino lambs of both sexes, with a live weight of 17 ± 3 kg, received an intratracheal inoculation of 20 ml saline solution containing 1.26 × 106 TCID50 BRSV (strain NMK-7) per ml. Lambs were slaughtered 1, 3, 7, 11 and 15 days postinoculation (DPI), and samples were taken for analysis using light-microscopic and immunohistochemical techniques. The results reflected the effect of the virus on airway epithelia. The presence of BRSV in ciliated bronchial and bronchiolar epithelial cells gave rise to cytopathological changes, including loss of cilia and cell necrosis; these changes might be expected to decrease the efficiency of mucociliary clearance, favouring the development of secondary bacterial bronchopneumonia. These results suggest a reduction in BRSV tropism for alveolar epithelia compared to bronchial and bronchiolar epithelia. Light-microscopic analysis revealed a narrowing of alveolar and airway lumina and a considerable interstitial inflammatory reaction. 2020-10-22T19:35:28Z 2020-10-22T19:35:28Z 1996 journal article http://hdl.handle.net/20.500.12792/5285 10.1111/j.1439-0450.1996.tb00310.x eng Attribution-NonCommercial-ShareAlike 4.0 International http://creativecommons.org/licenses/by-nc-sa/4.0/ open access |
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An experimental model was designed to characterize lesions in the lung of limbs inoculated with bovine respiratory syncytial virus (BRSV). Twenty-five Merino lambs of both sexes, with a live weight of 17 ± 3 kg, received an intratracheal inoculation of 20 ml saline solution containing 1.26 × 106 TCID50 BRSV (strain NMK-7) per ml. Lambs were slaughtered 1, 3, 7, 11 and 15 days postinoculation (DPI), and samples were taken for analysis using light-microscopic and immunohistochemical techniques. The results reflected the effect of the virus on airway epithelia. The presence of BRSV in ciliated bronchial and bronchiolar epithelial cells gave rise to cytopathological changes, including loss of cilia and cell necrosis; these changes might be expected to decrease the efficiency of mucociliary clearance, favouring the development of secondary bacterial bronchopneumonia. These results suggest a reduction in BRSV tropism for alveolar epithelia compared to bronchial and bronchiolar epithelia. Light-microscopic analysis revealed a narrowing of alveolar and airway lumina and a considerable interstitial inflammatory reaction. |
format |
journal article |
author |
Masot, A. J. Gomez-Tejedor, C. Gomez, L. Gazquez, A. Redondo, E. |
spellingShingle |
Masot, A. J. Gomez-Tejedor, C. Gomez, L. Gazquez, A. Redondo, E. Pathological study of experimentally induced bovine respiratory syncytial viral infection in lambs |
author_facet |
Masot, A. J. Gomez-Tejedor, C. Gomez, L. Gazquez, A. Redondo, E. |
author_sort |
Masot, A. J. |
title |
Pathological study of experimentally induced bovine respiratory syncytial viral infection in lambs |
title_short |
Pathological study of experimentally induced bovine respiratory syncytial viral infection in lambs |
title_full |
Pathological study of experimentally induced bovine respiratory syncytial viral infection in lambs |
title_fullStr |
Pathological study of experimentally induced bovine respiratory syncytial viral infection in lambs |
title_full_unstemmed |
Pathological study of experimentally induced bovine respiratory syncytial viral infection in lambs |
title_sort |
pathological study of experimentally induced bovine respiratory syncytial viral infection in lambs |
publishDate |
1996 |
url |
http://hdl.handle.net/20.500.12792/5285 |
work_keys_str_mv |
AT masotaj pathologicalstudyofexperimentallyinducedbovinerespiratorysyncytialviralinfectioninlambs AT gomeztejedorc pathologicalstudyofexperimentallyinducedbovinerespiratorysyncytialviralinfectioninlambs AT gomezl pathologicalstudyofexperimentallyinducedbovinerespiratorysyncytialviralinfectioninlambs AT gazqueza pathologicalstudyofexperimentallyinducedbovinerespiratorysyncytialviralinfectioninlambs AT redondoe pathologicalstudyofexperimentallyinducedbovinerespiratorysyncytialviralinfectioninlambs |
_version_ |
1758005045080621056 |