Selective lymphocyte depletion during the early stage of the immune response to foot-and-mouth disease virus infection in swine
Foot-and-mouth disease virus (FMDV) is the causative agent of a highly contagious vesicular disease of cloven-hoofed animals. In the present study we use FMDV serotype C infection of swine to determine, by analytical techniques, the direct ex vivo visualization of virus-infected immune cells during the first 17 days of infection. We report, for the first time, that FMDV C-S8c1 can infect T and B cells at short periods of time postinoculation, corresponding with the peak of the viremia. There is a significant lymphopenia that involves CD3+ CD4- CD8+/-, CD3+ CD4 - CD8+Tc, and CD3+ CD4+ CD8 + memory Th but not CD3+ CD4+ CD8- naïve Th lymphocytes. In addition, a profound depletion of the vast majority of peripheral T cells in lymph nodes and spleen is observed. This selective depletion of T cells is not due mainly to in situ death via apoptosis as visualized by the terminal deoxynucleotidyltransferase-mediated dUTP-biotin nick end labeling (TUNEL) technique. Thus, early infection of T cells by FMDV may be the main cause of the observed T-cell depletion. Importantly, this lack of T cells is reflected in a reduced response to mitogen activation, which in many cases is totally eliminated. These data suggest a mechanism by which the virus causes a transient immunosuppression, subvert the immune systems, and spreads. These results have important implications for our understanding of early events in the development of a robust immune response against FMDV. Copyright © 2006, American Society for Microbiology. All Rights Reserved.
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dig-inia-es-20.500.12792-50412020-12-15T09:46:41Z Selective lymphocyte depletion during the early stage of the immune response to foot-and-mouth disease virus infection in swine Díaz-San Segundo, F. Salguero, F. J. de Avila, A. Fernández de Marco, M. M. Sánchez-Martín, M. A. Sevilla, N. Foot-and-mouth disease virus (FMDV) is the causative agent of a highly contagious vesicular disease of cloven-hoofed animals. In the present study we use FMDV serotype C infection of swine to determine, by analytical techniques, the direct ex vivo visualization of virus-infected immune cells during the first 17 days of infection. We report, for the first time, that FMDV C-S8c1 can infect T and B cells at short periods of time postinoculation, corresponding with the peak of the viremia. There is a significant lymphopenia that involves CD3+ CD4- CD8+/-, CD3+ CD4 - CD8+Tc, and CD3+ CD4+ CD8 + memory Th but not CD3+ CD4+ CD8- naïve Th lymphocytes. In addition, a profound depletion of the vast majority of peripheral T cells in lymph nodes and spleen is observed. This selective depletion of T cells is not due mainly to in situ death via apoptosis as visualized by the terminal deoxynucleotidyltransferase-mediated dUTP-biotin nick end labeling (TUNEL) technique. Thus, early infection of T cells by FMDV may be the main cause of the observed T-cell depletion. Importantly, this lack of T cells is reflected in a reduced response to mitogen activation, which in many cases is totally eliminated. These data suggest a mechanism by which the virus causes a transient immunosuppression, subvert the immune systems, and spreads. These results have important implications for our understanding of early events in the development of a robust immune response against FMDV. Copyright © 2006, American Society for Microbiology. All Rights Reserved. 2020-10-22T18:49:59Z 2020-10-22T18:49:59Z 2006 journal article http://hdl.handle.net/20.500.12792/5041 10.1128/JVI.80.5.2369-2379.2006 eng Attribution-NonCommercial-ShareAlike 4.0 International http://creativecommons.org/licenses/by-nc-sa/4.0/ open access |
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Foot-and-mouth disease virus (FMDV) is the causative agent of a highly contagious vesicular disease of cloven-hoofed animals. In the present study we use FMDV serotype C infection of swine to determine, by analytical techniques, the direct ex vivo visualization of virus-infected immune cells during the first 17 days of infection. We report, for the first time, that FMDV C-S8c1 can infect T and B cells at short periods of time postinoculation, corresponding with the peak of the viremia. There is a significant lymphopenia that involves CD3+ CD4- CD8+/-, CD3+ CD4 - CD8+Tc, and CD3+ CD4+ CD8 + memory Th but not CD3+ CD4+ CD8- naïve Th lymphocytes. In addition, a profound depletion of the vast majority of peripheral T cells in lymph nodes and spleen is observed. This selective depletion of T cells is not due mainly to in situ death via apoptosis as visualized by the terminal deoxynucleotidyltransferase-mediated dUTP-biotin nick end labeling (TUNEL) technique. Thus, early infection of T cells by FMDV may be the main cause of the observed T-cell depletion. Importantly, this lack of T cells is reflected in a reduced response to mitogen activation, which in many cases is totally eliminated. These data suggest a mechanism by which the virus causes a transient immunosuppression, subvert the immune systems, and spreads. These results have important implications for our understanding of early events in the development of a robust immune response against FMDV. Copyright © 2006, American Society for Microbiology. All Rights Reserved. |
format |
journal article |
author |
Díaz-San Segundo, F. Salguero, F. J. de Avila, A. Fernández de Marco, M. M. Sánchez-Martín, M. A. Sevilla, N. |
spellingShingle |
Díaz-San Segundo, F. Salguero, F. J. de Avila, A. Fernández de Marco, M. M. Sánchez-Martín, M. A. Sevilla, N. Selective lymphocyte depletion during the early stage of the immune response to foot-and-mouth disease virus infection in swine |
author_facet |
Díaz-San Segundo, F. Salguero, F. J. de Avila, A. Fernández de Marco, M. M. Sánchez-Martín, M. A. Sevilla, N. |
author_sort |
Díaz-San Segundo, F. |
title |
Selective lymphocyte depletion during the early stage of the immune response to foot-and-mouth disease virus infection in swine |
title_short |
Selective lymphocyte depletion during the early stage of the immune response to foot-and-mouth disease virus infection in swine |
title_full |
Selective lymphocyte depletion during the early stage of the immune response to foot-and-mouth disease virus infection in swine |
title_fullStr |
Selective lymphocyte depletion during the early stage of the immune response to foot-and-mouth disease virus infection in swine |
title_full_unstemmed |
Selective lymphocyte depletion during the early stage of the immune response to foot-and-mouth disease virus infection in swine |
title_sort |
selective lymphocyte depletion during the early stage of the immune response to foot-and-mouth disease virus infection in swine |
publishDate |
2006 |
url |
http://hdl.handle.net/20.500.12792/5041 |
work_keys_str_mv |
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