Effect of the pH pre-adjustment in the freezing extender on post-thaw boar sperm quality

Effect of the pH pre-adjustment in the freezing extender on post-thaw boar sperm quality

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BACKGROUND During freezing the selective precipitation of substances in the medium may provoke a pH shift and lead to sperm damage. OBJECTIVE To study the effect of the pH pre-adjustment in the freezing extender on post-thaw boar sperm quality. METHODS A total of 15 ejaculates from different boars were obtained and divided into six aliquots prior to a standard straw cryopreservation in freezing extender (lactose-egg yolk-glycerol-Orvus ES Paste) with different pH. After thawing, sperm quality (plasma membrane integrity, motility and acrosome status) were assessed at 30 and 90 minutes of post-thaw incubation at 37 degree C. RESULTS When the boar sperm were frozen in a freezing media with pH basic, and particularly at pH 8, it had higher post-thaw sperm quality. CONCLUSION The pre-adjustment at pH 8 of the freezing extender (lactose-egg yolk-glycerol-Orvus ES Paste) is able to improve the post-thaw boar sperm quality.

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Main Authors: Tomas, C., Gómez-Fernandez, J., Gómez-Izquierdo, E., Gómez-Fidalgo, E., Sánchez-Sánchez, R., González-Bulnes, A., de Mercado, E.
Format: journal article biblioteca
Language:eng
Published: 2015
Online Access:http://hdl.handle.net/20.500.12792/4499
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spelling dig-inia-es-20.500.12792-44992020-12-15T09:46:44Z Effect of the pH pre-adjustment in the freezing extender on post-thaw boar sperm quality Tomas, C. Gómez-Fernandez, J. Gómez-Izquierdo, E. Gómez-Fidalgo, E. Sánchez-Sánchez, R. González-Bulnes, A. de Mercado, E. BACKGROUND During freezing the selective precipitation of substances in the medium may provoke a pH shift and lead to sperm damage. OBJECTIVE To study the effect of the pH pre-adjustment in the freezing extender on post-thaw boar sperm quality. METHODS A total of 15 ejaculates from different boars were obtained and divided into six aliquots prior to a standard straw cryopreservation in freezing extender (lactose-egg yolk-glycerol-Orvus ES Paste) with different pH. After thawing, sperm quality (plasma membrane integrity, motility and acrosome status) were assessed at 30 and 90 minutes of post-thaw incubation at 37 degree C. RESULTS When the boar sperm were frozen in a freezing media with pH basic, and particularly at pH 8, it had higher post-thaw sperm quality. CONCLUSION The pre-adjustment at pH 8 of the freezing extender (lactose-egg yolk-glycerol-Orvus ES Paste) is able to improve the post-thaw boar sperm quality. 2020-10-22T17:15:14Z 2020-10-22T17:15:14Z 2015 journal article http://hdl.handle.net/20.500.12792/4499 eng Attribution-NonCommercial-ShareAlike 4.0 International http://creativecommons.org/licenses/by-nc-sa/4.0/ open access
institution INIA ES
collection DSpace
country España
countrycode ES
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databasecode dig-inia-es
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region Europa del Sur
libraryname Biblioteca del INIA España
language eng
description BACKGROUND During freezing the selective precipitation of substances in the medium may provoke a pH shift and lead to sperm damage. OBJECTIVE To study the effect of the pH pre-adjustment in the freezing extender on post-thaw boar sperm quality. METHODS A total of 15 ejaculates from different boars were obtained and divided into six aliquots prior to a standard straw cryopreservation in freezing extender (lactose-egg yolk-glycerol-Orvus ES Paste) with different pH. After thawing, sperm quality (plasma membrane integrity, motility and acrosome status) were assessed at 30 and 90 minutes of post-thaw incubation at 37 degree C. RESULTS When the boar sperm were frozen in a freezing media with pH basic, and particularly at pH 8, it had higher post-thaw sperm quality. CONCLUSION The pre-adjustment at pH 8 of the freezing extender (lactose-egg yolk-glycerol-Orvus ES Paste) is able to improve the post-thaw boar sperm quality.
format journal article
author Tomas, C.
Gómez-Fernandez, J.
Gómez-Izquierdo, E.
Gómez-Fidalgo, E.
Sánchez-Sánchez, R.
González-Bulnes, A.
de Mercado, E.
spellingShingle Tomas, C.
Gómez-Fernandez, J.
Gómez-Izquierdo, E.
Gómez-Fidalgo, E.
Sánchez-Sánchez, R.
González-Bulnes, A.
de Mercado, E.
Effect of the pH pre-adjustment in the freezing extender on post-thaw boar sperm quality
author_facet Tomas, C.
Gómez-Fernandez, J.
Gómez-Izquierdo, E.
Gómez-Fidalgo, E.
Sánchez-Sánchez, R.
González-Bulnes, A.
de Mercado, E.
author_sort Tomas, C.
title Effect of the pH pre-adjustment in the freezing extender on post-thaw boar sperm quality
title_short Effect of the pH pre-adjustment in the freezing extender on post-thaw boar sperm quality
title_full Effect of the pH pre-adjustment in the freezing extender on post-thaw boar sperm quality
title_fullStr Effect of the pH pre-adjustment in the freezing extender on post-thaw boar sperm quality
title_full_unstemmed Effect of the pH pre-adjustment in the freezing extender on post-thaw boar sperm quality
title_sort effect of the ph pre-adjustment in the freezing extender on post-thaw boar sperm quality
publishDate 2015
url http://hdl.handle.net/20.500.12792/4499
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