Evidence of the coevolution of antigenicity and host cell tropism of foot-and-mouth disease virus in vivo

Evidence of the coevolution of antigenicity and host cell tropism of foot-and-mouth disease virus in vivo

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In this work we analyze the antigenic properties and the stability in cell culture of virus mutants recovered upon challenge of peptide-vaccinated cattle with foot-and-mouth disease virus (FMDV) C3 Arg85. Previously, we showed that a significant proportion of 29 lesions analyzed (41%) contained viruses with single amino acid replacements (R141G, L144P, or L147P) within a major antigenic site located at the G-H loop of VP1, known to participate also in interactions with integrin receptors. Here we document that no replacements at this site were found in viruses from 12 lesions developed in six control animals upon challenge with FMDV C3 Arg85. Sera from unprotected, vaccinated animals exhibited poor neutralization titers against mutants recovered from them. Sequence analyses of the viruses recovered upon 10 serial passages in BHK-21 and FBK-2 cells in the presence of preimmune (nonneutralizing) sera revealed that mutants reverted to the parental sequence, suggesting an effect of the amino acid replacements in the interaction of the viruses with cells. Parallel passages in the presence of subneutralizing concentrations of immune homologous sera resulted in the maintenance of mutations R141G and L147P, while mutation L144P reverted to the C3 Arg85 sequence. Reactivity with a panel of FMDV type C-specific monoclonal antibodies indicated that mutant viruses showed altered antigenicity. These results suggest that the selective pressure exerted by host humoral immune response can play a role in both the selection and stability of antigenic FMDV variants and that such variants can manifest alterations in cell tropism.

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Main Authors: Tami, C., Taboga, O., Berinstein, A., Núñez, J. I., Palma, E. L., Domingo, E., Sobrino, F., Carrillo, E.
Format: journal article biblioteca
Language:eng
Published: 2003
Online Access:http://hdl.handle.net/20.500.12792/4439
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spelling dig-inia-es-20.500.12792-44392020-12-15T09:54:35Z Evidence of the coevolution of antigenicity and host cell tropism of foot-and-mouth disease virus in vivo Tami, C. Taboga, O. Berinstein, A. Núñez, J. I. Palma, E. L. Domingo, E. Sobrino, F. Carrillo, E. In this work we analyze the antigenic properties and the stability in cell culture of virus mutants recovered upon challenge of peptide-vaccinated cattle with foot-and-mouth disease virus (FMDV) C3 Arg85. Previously, we showed that a significant proportion of 29 lesions analyzed (41%) contained viruses with single amino acid replacements (R141G, L144P, or L147P) within a major antigenic site located at the G-H loop of VP1, known to participate also in interactions with integrin receptors. Here we document that no replacements at this site were found in viruses from 12 lesions developed in six control animals upon challenge with FMDV C3 Arg85. Sera from unprotected, vaccinated animals exhibited poor neutralization titers against mutants recovered from them. Sequence analyses of the viruses recovered upon 10 serial passages in BHK-21 and FBK-2 cells in the presence of preimmune (nonneutralizing) sera revealed that mutants reverted to the parental sequence, suggesting an effect of the amino acid replacements in the interaction of the viruses with cells. Parallel passages in the presence of subneutralizing concentrations of immune homologous sera resulted in the maintenance of mutations R141G and L147P, while mutation L144P reverted to the C3 Arg85 sequence. Reactivity with a panel of FMDV type C-specific monoclonal antibodies indicated that mutant viruses showed altered antigenicity. These results suggest that the selective pressure exerted by host humoral immune response can play a role in both the selection and stability of antigenic FMDV variants and that such variants can manifest alterations in cell tropism. 2020-10-22T17:05:30Z 2020-10-22T17:05:30Z 2003 journal article http://hdl.handle.net/20.500.12792/4439 10.1128/JVI.77.2.1219-1226.2003 eng Attribution-NonCommercial-ShareAlike 4.0 International http://creativecommons.org/licenses/by-nc-sa/4.0/ open access
institution INIA ES
collection DSpace
country España
countrycode ES
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libraryname Biblioteca del INIA España
language eng
description In this work we analyze the antigenic properties and the stability in cell culture of virus mutants recovered upon challenge of peptide-vaccinated cattle with foot-and-mouth disease virus (FMDV) C3 Arg85. Previously, we showed that a significant proportion of 29 lesions analyzed (41%) contained viruses with single amino acid replacements (R141G, L144P, or L147P) within a major antigenic site located at the G-H loop of VP1, known to participate also in interactions with integrin receptors. Here we document that no replacements at this site were found in viruses from 12 lesions developed in six control animals upon challenge with FMDV C3 Arg85. Sera from unprotected, vaccinated animals exhibited poor neutralization titers against mutants recovered from them. Sequence analyses of the viruses recovered upon 10 serial passages in BHK-21 and FBK-2 cells in the presence of preimmune (nonneutralizing) sera revealed that mutants reverted to the parental sequence, suggesting an effect of the amino acid replacements in the interaction of the viruses with cells. Parallel passages in the presence of subneutralizing concentrations of immune homologous sera resulted in the maintenance of mutations R141G and L147P, while mutation L144P reverted to the C3 Arg85 sequence. Reactivity with a panel of FMDV type C-specific monoclonal antibodies indicated that mutant viruses showed altered antigenicity. These results suggest that the selective pressure exerted by host humoral immune response can play a role in both the selection and stability of antigenic FMDV variants and that such variants can manifest alterations in cell tropism.
format journal article
author Tami, C.
Taboga, O.
Berinstein, A.
Núñez, J. I.
Palma, E. L.
Domingo, E.
Sobrino, F.
Carrillo, E.
spellingShingle Tami, C.
Taboga, O.
Berinstein, A.
Núñez, J. I.
Palma, E. L.
Domingo, E.
Sobrino, F.
Carrillo, E.
Evidence of the coevolution of antigenicity and host cell tropism of foot-and-mouth disease virus in vivo
author_facet Tami, C.
Taboga, O.
Berinstein, A.
Núñez, J. I.
Palma, E. L.
Domingo, E.
Sobrino, F.
Carrillo, E.
author_sort Tami, C.
title Evidence of the coevolution of antigenicity and host cell tropism of foot-and-mouth disease virus in vivo
title_short Evidence of the coevolution of antigenicity and host cell tropism of foot-and-mouth disease virus in vivo
title_full Evidence of the coevolution of antigenicity and host cell tropism of foot-and-mouth disease virus in vivo
title_fullStr Evidence of the coevolution of antigenicity and host cell tropism of foot-and-mouth disease virus in vivo
title_full_unstemmed Evidence of the coevolution of antigenicity and host cell tropism of foot-and-mouth disease virus in vivo
title_sort evidence of the coevolution of antigenicity and host cell tropism of foot-and-mouth disease virus in vivo
publishDate 2003
url http://hdl.handle.net/20.500.12792/4439
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