Validating a commercially available enzyme immunoassay for the determination of 17β-estradiol and progestogens in the feces of cheetahs (Acinonyx jubatus) A case report
Fecal 17β-estradiol and progestogens excretion was monitored in adult, female cheetahs (Acinonyx jubatus; n = 2), ZGG-12301 (born 3 April 1993), gonadotrophin treated and ZGT-3301, (born 19 August 1993), nontreated, for 120 days using commercially available plate enzyme immunoassay kits prepared for human serum or plasma. There were significant differences (P < 0.001) between baseline and peak concentrations of both hormone measures. Female ZGG-12301, which conceived, but this pregnancy resulted in an unobserved spontaneous abortion, showed no significant difference (P > 0.05) between baseline and gestation 17β-estradiol values; fecal 17β-estradiol excretion during pregnancy was statistically different (P < 0.001) from excretion during the nonpregnancy period. Baseline progestogen concentrations were different from pregnancy (P < 0.001) and postovulatory (P < 0.01) concentrations, and progestogen concentrations during pregnancy period were different (P < 0.001) from postovulatory concentrations. In the nontreated cheetah (ZGT-3301), basal and increased progestogen concentrations were statistically different (P < 0.01). On the basis of 17β-estradiol excretory patterns, duration of the estrous cycle (x- ± SEM) was 13.2 ± 2.2 days. These results suggest that the enzyme-linked immunosorbent methods reported in this study were capable of quantifying reproductive hormones in fecal extracts of cheetahs and could be a practical alternative to other enzyme-linked immunosorbent assays which require more complex procedures. Copyright 2005 by American Association of Zoo Veterinarians.
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dig-inia-es-20.500.12792-41512020-12-15T09:54:33Z Validating a commercially available enzyme immunoassay for the determination of 17β-estradiol and progestogens in the feces of cheetahs (Acinonyx jubatus) A case report Borque, C. Perez-Garnelo, S. S. Lopez, M. Talavera, C. Delclaux, M. De La Fuente, J. Fecal 17β-estradiol and progestogens excretion was monitored in adult, female cheetahs (Acinonyx jubatus; n = 2), ZGG-12301 (born 3 April 1993), gonadotrophin treated and ZGT-3301, (born 19 August 1993), nontreated, for 120 days using commercially available plate enzyme immunoassay kits prepared for human serum or plasma. There were significant differences (P < 0.001) between baseline and peak concentrations of both hormone measures. Female ZGG-12301, which conceived, but this pregnancy resulted in an unobserved spontaneous abortion, showed no significant difference (P > 0.05) between baseline and gestation 17β-estradiol values; fecal 17β-estradiol excretion during pregnancy was statistically different (P < 0.001) from excretion during the nonpregnancy period. Baseline progestogen concentrations were different from pregnancy (P < 0.001) and postovulatory (P < 0.01) concentrations, and progestogen concentrations during pregnancy period were different (P < 0.001) from postovulatory concentrations. In the nontreated cheetah (ZGT-3301), basal and increased progestogen concentrations were statistically different (P < 0.01). On the basis of 17β-estradiol excretory patterns, duration of the estrous cycle (x- ± SEM) was 13.2 ± 2.2 days. These results suggest that the enzyme-linked immunosorbent methods reported in this study were capable of quantifying reproductive hormones in fecal extracts of cheetahs and could be a practical alternative to other enzyme-linked immunosorbent assays which require more complex procedures. Copyright 2005 by American Association of Zoo Veterinarians. 2020-10-22T16:19:25Z 2020-10-22T16:19:25Z 2005 journal article http://hdl.handle.net/20.500.12792/4151 10.1638/03-068 eng Attribution-NonCommercial-ShareAlike 4.0 International http://creativecommons.org/licenses/by-nc-sa/4.0/ open access |
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Fecal 17β-estradiol and progestogens excretion was monitored in adult, female cheetahs (Acinonyx jubatus; n = 2), ZGG-12301 (born 3 April 1993), gonadotrophin treated and ZGT-3301, (born 19 August 1993), nontreated, for 120 days using commercially available plate enzyme immunoassay kits prepared for human serum or plasma. There were significant differences (P < 0.001) between baseline and peak concentrations of both hormone measures. Female ZGG-12301, which conceived, but this pregnancy resulted in an unobserved spontaneous abortion, showed no significant difference (P > 0.05) between baseline and gestation 17β-estradiol values; fecal 17β-estradiol excretion during pregnancy was statistically different (P < 0.001) from excretion during the nonpregnancy period. Baseline progestogen concentrations were different from pregnancy (P < 0.001) and postovulatory (P < 0.01) concentrations, and progestogen concentrations during pregnancy period were different (P < 0.001) from postovulatory concentrations. In the nontreated cheetah (ZGT-3301), basal and increased progestogen concentrations were statistically different (P < 0.01). On the basis of 17β-estradiol excretory patterns, duration of the estrous cycle (x- ± SEM) was 13.2 ± 2.2 days. These results suggest that the enzyme-linked immunosorbent methods reported in this study were capable of quantifying reproductive hormones in fecal extracts of cheetahs and could be a practical alternative to other enzyme-linked immunosorbent assays which require more complex procedures. Copyright 2005 by American Association of Zoo Veterinarians. |
| format |
journal article |
| author |
Borque, C. Perez-Garnelo, S. S. Lopez, M. Talavera, C. Delclaux, M. De La Fuente, J. |
| spellingShingle |
Borque, C. Perez-Garnelo, S. S. Lopez, M. Talavera, C. Delclaux, M. De La Fuente, J. Validating a commercially available enzyme immunoassay for the determination of 17β-estradiol and progestogens in the feces of cheetahs (Acinonyx jubatus) A case report |
| author_facet |
Borque, C. Perez-Garnelo, S. S. Lopez, M. Talavera, C. Delclaux, M. De La Fuente, J. |
| author_sort |
Borque, C. |
| title |
Validating a commercially available enzyme immunoassay for the determination of 17β-estradiol and progestogens in the feces of cheetahs (Acinonyx jubatus) A case report |
| title_short |
Validating a commercially available enzyme immunoassay for the determination of 17β-estradiol and progestogens in the feces of cheetahs (Acinonyx jubatus) A case report |
| title_full |
Validating a commercially available enzyme immunoassay for the determination of 17β-estradiol and progestogens in the feces of cheetahs (Acinonyx jubatus) A case report |
| title_fullStr |
Validating a commercially available enzyme immunoassay for the determination of 17β-estradiol and progestogens in the feces of cheetahs (Acinonyx jubatus) A case report |
| title_full_unstemmed |
Validating a commercially available enzyme immunoassay for the determination of 17β-estradiol and progestogens in the feces of cheetahs (Acinonyx jubatus) A case report |
| title_sort |
validating a commercially available enzyme immunoassay for the determination of 17β-estradiol and progestogens in the feces of cheetahs (acinonyx jubatus) a case report |
| publishDate |
2005 |
| url |
http://hdl.handle.net/20.500.12792/4151 |
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