Quantification of low levels of rainbow trout immunoglobulin by enzyme immunoassay using two monoclonal antibodies
An enzyme immunoassay has been developed for quantitation of low levels of trout immunoglobulin (Ig). This assay uses two monoclonal antibodies, one as capture antibody and the other as detector, directed against two non-overlapping epitopes on the heavy chains of trout Ig. The assay shows high reproducibility and can detect 0.12 μg trout Ig ml-1. Coefficients of intra- and interassay variation ranged from 3.8 to 7.1% and from 7.9 to 17.4%, respectively. Analysis of 37 healthy trout showed increasing serum Ig concentration with size. The mean Ig concentration was 0.67 mg ml-1 for trout of about 20 g and 9.1 mg ml-1 for trout weighing more than 125 g. © 1993.
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1993
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dig-inia-es-20.500.12792-40152020-12-15T09:54:33Z Quantification of low levels of rainbow trout immunoglobulin by enzyme immunoassay using two monoclonal antibodies Sánchez, C. Babin, M. Tomillo, J. Ubeira, F. M. Domínguez, J. An enzyme immunoassay has been developed for quantitation of low levels of trout immunoglobulin (Ig). This assay uses two monoclonal antibodies, one as capture antibody and the other as detector, directed against two non-overlapping epitopes on the heavy chains of trout Ig. The assay shows high reproducibility and can detect 0.12 μg trout Ig ml-1. Coefficients of intra- and interassay variation ranged from 3.8 to 7.1% and from 7.9 to 17.4%, respectively. Analysis of 37 healthy trout showed increasing serum Ig concentration with size. The mean Ig concentration was 0.67 mg ml-1 for trout of about 20 g and 9.1 mg ml-1 for trout weighing more than 125 g. © 1993. 2020-10-22T16:00:02Z 2020-10-22T16:00:02Z 1993 journal article http://hdl.handle.net/20.500.12792/4015 10.1016/0165-2427(93)90006-P eng Attribution-NonCommercial-ShareAlike 4.0 International http://creativecommons.org/licenses/by-nc-sa/4.0/ open access |
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An enzyme immunoassay has been developed for quantitation of low levels of trout immunoglobulin (Ig). This assay uses two monoclonal antibodies, one as capture antibody and the other as detector, directed against two non-overlapping epitopes on the heavy chains of trout Ig. The assay shows high reproducibility and can detect 0.12 μg trout Ig ml-1. Coefficients of intra- and interassay variation ranged from 3.8 to 7.1% and from 7.9 to 17.4%, respectively. Analysis of 37 healthy trout showed increasing serum Ig concentration with size. The mean Ig concentration was 0.67 mg ml-1 for trout of about 20 g and 9.1 mg ml-1 for trout weighing more than 125 g. © 1993. |
| format |
journal article |
| author |
Sánchez, C. Babin, M. Tomillo, J. Ubeira, F. M. Domínguez, J. |
| spellingShingle |
Sánchez, C. Babin, M. Tomillo, J. Ubeira, F. M. Domínguez, J. Quantification of low levels of rainbow trout immunoglobulin by enzyme immunoassay using two monoclonal antibodies |
| author_facet |
Sánchez, C. Babin, M. Tomillo, J. Ubeira, F. M. Domínguez, J. |
| author_sort |
Sánchez, C. |
| title |
Quantification of low levels of rainbow trout immunoglobulin by enzyme immunoassay using two monoclonal antibodies |
| title_short |
Quantification of low levels of rainbow trout immunoglobulin by enzyme immunoassay using two monoclonal antibodies |
| title_full |
Quantification of low levels of rainbow trout immunoglobulin by enzyme immunoassay using two monoclonal antibodies |
| title_fullStr |
Quantification of low levels of rainbow trout immunoglobulin by enzyme immunoassay using two monoclonal antibodies |
| title_full_unstemmed |
Quantification of low levels of rainbow trout immunoglobulin by enzyme immunoassay using two monoclonal antibodies |
| title_sort |
quantification of low levels of rainbow trout immunoglobulin by enzyme immunoassay using two monoclonal antibodies |
| publishDate |
1993 |
| url |
http://hdl.handle.net/20.500.12792/4015 |
| work_keys_str_mv |
AT sanchezc quantificationoflowlevelsofrainbowtroutimmunoglobulinbyenzymeimmunoassayusingtwomonoclonalantibodies AT babinm quantificationoflowlevelsofrainbowtroutimmunoglobulinbyenzymeimmunoassayusingtwomonoclonalantibodies AT tomilloj quantificationoflowlevelsofrainbowtroutimmunoglobulinbyenzymeimmunoassayusingtwomonoclonalantibodies AT ubeirafm quantificationoflowlevelsofrainbowtroutimmunoglobulinbyenzymeimmunoassayusingtwomonoclonalantibodies AT dominguezj quantificationoflowlevelsofrainbowtroutimmunoglobulinbyenzymeimmunoassayusingtwomonoclonalantibodies |
| _version_ |
1758004855895490560 |