DNA methylation increases throughout Arabidopsis development
We used amplified fragment length polymorphisms (AFLP) to analyze the stability of DNA methylation throughout Arabidopsis development. AFLP can detect genome-wide changes in cytosine methylation produced by DNA demethylation agents, such as 5-azacytidine, or specific mutations at the DDM1 locus. In both cases, cytosine demethylation is associated with a general increase in the presence of amplified fragments. Using this approach, we followed DNA methylation at methylation sensitive restriction sites throughout Arabidopsis development. The results show a progressive DNA methylation trend from cotyledons to vegetative organs to reproductive organs. © Springer-Verlag 2005.
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Main Authors: | Ruiz-García, L., Cervera, M. T., Martínez-Zapater, J. M. |
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Format: | journal article biblioteca |
Language: | eng |
Published: |
2005
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Online Access: | http://hdl.handle.net/20.500.12792/3934 |
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