DNA methylation increases throughout Arabidopsis development
We used amplified fragment length polymorphisms (AFLP) to analyze the stability of DNA methylation throughout Arabidopsis development. AFLP can detect genome-wide changes in cytosine methylation produced by DNA demethylation agents, such as 5-azacytidine, or specific mutations at the DDM1 locus. In both cases, cytosine demethylation is associated with a general increase in the presence of amplified fragments. Using this approach, we followed DNA methylation at methylation sensitive restriction sites throughout Arabidopsis development. The results show a progressive DNA methylation trend from cotyledons to vegetative organs to reproductive organs. © Springer-Verlag 2005.
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dig-inia-es-20.500.12792-39342020-12-15T09:54:32Z DNA methylation increases throughout Arabidopsis development Ruiz-García, L. Cervera, M. T. Martínez-Zapater, J. M. We used amplified fragment length polymorphisms (AFLP) to analyze the stability of DNA methylation throughout Arabidopsis development. AFLP can detect genome-wide changes in cytosine methylation produced by DNA demethylation agents, such as 5-azacytidine, or specific mutations at the DDM1 locus. In both cases, cytosine demethylation is associated with a general increase in the presence of amplified fragments. Using this approach, we followed DNA methylation at methylation sensitive restriction sites throughout Arabidopsis development. The results show a progressive DNA methylation trend from cotyledons to vegetative organs to reproductive organs. © Springer-Verlag 2005. 2020-10-22T15:47:50Z 2020-10-22T15:47:50Z 2005 journal article http://hdl.handle.net/20.500.12792/3934 10.1007/s00425-005-1524-6 eng Attribution-NonCommercial-ShareAlike 4.0 International http://creativecommons.org/licenses/by-nc-sa/4.0/ open access |
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We used amplified fragment length polymorphisms (AFLP) to analyze the stability of DNA methylation throughout Arabidopsis development. AFLP can detect genome-wide changes in cytosine methylation produced by DNA demethylation agents, such as 5-azacytidine, or specific mutations at the DDM1 locus. In both cases, cytosine demethylation is associated with a general increase in the presence of amplified fragments. Using this approach, we followed DNA methylation at methylation sensitive restriction sites throughout Arabidopsis development. The results show a progressive DNA methylation trend from cotyledons to vegetative organs to reproductive organs. © Springer-Verlag 2005. |
| format |
journal article |
| author |
Ruiz-García, L. Cervera, M. T. Martínez-Zapater, J. M. |
| spellingShingle |
Ruiz-García, L. Cervera, M. T. Martínez-Zapater, J. M. DNA methylation increases throughout Arabidopsis development |
| author_facet |
Ruiz-García, L. Cervera, M. T. Martínez-Zapater, J. M. |
| author_sort |
Ruiz-García, L. |
| title |
DNA methylation increases throughout Arabidopsis development |
| title_short |
DNA methylation increases throughout Arabidopsis development |
| title_full |
DNA methylation increases throughout Arabidopsis development |
| title_fullStr |
DNA methylation increases throughout Arabidopsis development |
| title_full_unstemmed |
DNA methylation increases throughout Arabidopsis development |
| title_sort |
dna methylation increases throughout arabidopsis development |
| publishDate |
2005 |
| url |
http://hdl.handle.net/20.500.12792/3934 |
| work_keys_str_mv |
AT ruizgarcial dnamethylationincreasesthroughoutarabidopsisdevelopment AT cerveramt dnamethylationincreasesthroughoutarabidopsisdevelopment AT martinezzapaterjm dnamethylationincreasesthroughoutarabidopsisdevelopment |
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1758004723321929728 |