Attenuated infectious hematopoietic necrosis virus with rearranged gene order as potential vaccine
The genome of infectious hematopoietic necrosis virus (IHNV), a salmonid novirhabdovirus, has been engineered to modify the gene order and to evaluate the impact on a possible attenuation of the virus in vitro and in vivo. By reverse genetics, eight recombinant IHNVs (rIHNVs), termed NxGy according to the respective positions of the nucleoprotein (N) and glycoprotein (G) genes along the genome, have been recovered. All rIHNVs have been fully characterized in vitro for their cytopathic effects, kinetics of replication, and profiles of viral gene transcription. These rIHNVs are stable through up to 10 passages in cell culture. Following bath immersion administration of the various rIHNVs to juvenile trout, some of the rIHNVs were clearly attenuated (N2G3, N2G4, N3G4, and N4G1). The position of the N gene seems to be one of the most critical features correlated to the level of viral attenuation. The induced immune response potential in fish was evaluated by enzyme-linked immunosorbent spot assay (ELISPOT) and seroneutralization assays. The recombinant virus N2G3 induced a strong antibody response in immunized fish and conferred 86% of protection against wild-type IHNV challenge in trout, thus representing a promising starting point for the development of a live attenuated vaccine candidate. © 2016, American Society for Microbiology. All Rights Reserved.
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dig-inia-es-20.500.12792-38942020-12-15T09:14:37Z Attenuated infectious hematopoietic necrosis virus with rearranged gene order as potential vaccine Rouxel, R. N. Tafalla, C. Mérour, E. Leal, E. Biacchesi, S. Brémont, M. The genome of infectious hematopoietic necrosis virus (IHNV), a salmonid novirhabdovirus, has been engineered to modify the gene order and to evaluate the impact on a possible attenuation of the virus in vitro and in vivo. By reverse genetics, eight recombinant IHNVs (rIHNVs), termed NxGy according to the respective positions of the nucleoprotein (N) and glycoprotein (G) genes along the genome, have been recovered. All rIHNVs have been fully characterized in vitro for their cytopathic effects, kinetics of replication, and profiles of viral gene transcription. These rIHNVs are stable through up to 10 passages in cell culture. Following bath immersion administration of the various rIHNVs to juvenile trout, some of the rIHNVs were clearly attenuated (N2G3, N2G4, N3G4, and N4G1). The position of the N gene seems to be one of the most critical features correlated to the level of viral attenuation. The induced immune response potential in fish was evaluated by enzyme-linked immunosorbent spot assay (ELISPOT) and seroneutralization assays. The recombinant virus N2G3 induced a strong antibody response in immunized fish and conferred 86% of protection against wild-type IHNV challenge in trout, thus representing a promising starting point for the development of a live attenuated vaccine candidate. © 2016, American Society for Microbiology. All Rights Reserved. 2020-10-22T15:42:13Z 2020-10-22T15:42:13Z 2016 journal article http://hdl.handle.net/20.500.12792/3894 10.1128/JVI.01024-16 eng Attribution-NonCommercial-ShareAlike 4.0 International http://creativecommons.org/licenses/by-nc-sa/4.0/ open access |
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The genome of infectious hematopoietic necrosis virus (IHNV), a salmonid novirhabdovirus, has been engineered to modify the gene order and to evaluate the impact on a possible attenuation of the virus in vitro and in vivo. By reverse genetics, eight recombinant IHNVs (rIHNVs), termed NxGy according to the respective positions of the nucleoprotein (N) and glycoprotein (G) genes along the genome, have been recovered. All rIHNVs have been fully characterized in vitro for their cytopathic effects, kinetics of replication, and profiles of viral gene transcription. These rIHNVs are stable through up to 10 passages in cell culture. Following bath immersion administration of the various rIHNVs to juvenile trout, some of the rIHNVs were clearly attenuated (N2G3, N2G4, N3G4, and N4G1). The position of the N gene seems to be one of the most critical features correlated to the level of viral attenuation. The induced immune response potential in fish was evaluated by enzyme-linked immunosorbent spot assay (ELISPOT) and seroneutralization assays. The recombinant virus N2G3 induced a strong antibody response in immunized fish and conferred 86% of protection against wild-type IHNV challenge in trout, thus representing a promising starting point for the development of a live attenuated vaccine candidate. © 2016, American Society for Microbiology. All Rights Reserved. |
format |
journal article |
author |
Rouxel, R. N. Tafalla, C. Mérour, E. Leal, E. Biacchesi, S. Brémont, M. |
spellingShingle |
Rouxel, R. N. Tafalla, C. Mérour, E. Leal, E. Biacchesi, S. Brémont, M. Attenuated infectious hematopoietic necrosis virus with rearranged gene order as potential vaccine |
author_facet |
Rouxel, R. N. Tafalla, C. Mérour, E. Leal, E. Biacchesi, S. Brémont, M. |
author_sort |
Rouxel, R. N. |
title |
Attenuated infectious hematopoietic necrosis virus with rearranged gene order as potential vaccine |
title_short |
Attenuated infectious hematopoietic necrosis virus with rearranged gene order as potential vaccine |
title_full |
Attenuated infectious hematopoietic necrosis virus with rearranged gene order as potential vaccine |
title_fullStr |
Attenuated infectious hematopoietic necrosis virus with rearranged gene order as potential vaccine |
title_full_unstemmed |
Attenuated infectious hematopoietic necrosis virus with rearranged gene order as potential vaccine |
title_sort |
attenuated infectious hematopoietic necrosis virus with rearranged gene order as potential vaccine |
publishDate |
2016 |
url |
http://hdl.handle.net/20.500.12792/3894 |
work_keys_str_mv |
AT rouxelrn attenuatedinfectioushematopoieticnecrosisviruswithrearrangedgeneorderaspotentialvaccine AT tafallac attenuatedinfectioushematopoieticnecrosisviruswithrearrangedgeneorderaspotentialvaccine AT meroure attenuatedinfectioushematopoieticnecrosisviruswithrearrangedgeneorderaspotentialvaccine AT leale attenuatedinfectioushematopoieticnecrosisviruswithrearrangedgeneorderaspotentialvaccine AT biacchesis attenuatedinfectioushematopoieticnecrosisviruswithrearrangedgeneorderaspotentialvaccine AT bremontm attenuatedinfectioushematopoieticnecrosisviruswithrearrangedgeneorderaspotentialvaccine |
_version_ |
1758005053975691264 |