Sex determines the expression level of one third of the actively expressed genes in bovine blastocysts
Although genetically identical for autosomal Chrs (Chr), male and female preimplantation embryos could display sex-specific transcriptional regulation. To illustrate sex-specific differences at the mRNA level, we compared gene-expression patterns between male and female blastocysts by DNA microarray comparison of nine groups of 60 bovine in vitro-produced blastocysts of each sex. Almost one-third of the transcripts detected showed sexual dimorphism (2,921 transcripts; false-discovery rate, P < 0.05), suggesting that in the absence of hormonal influences, the sex Chrs impose an extensive transcriptional regulation upon autosomal genes. Six geneswere analyzed by qPCR in in vivo-derived embryos, which displayed similar sexual dimorphism. Ontology analysis suggested a higher global transcriptional level in females andamore active proteinmetabolisminmales. Agenehomologto an X-linkedgeneinvolved in network interactions during spliceosome assembly was found in the Y-Chr. Most of the X-linked-expressed transcripts (88.5%)were up-regulated in females, but most of them (70%) exhibited fold-changes lower than 1.6, suggesting that X-Chr inactivation is partially achieved at the blastocyst stage. Almost half of the transcripts up-regulated in female embryos exhibiting more than 1.6-fold change were present in the X-Chr and eight of themwere selected to determinea putativepaternal imprinting by gene expression comparison with parthenogenetic embryos. Five (BEX, CAPN6, BEX2, SRPX2, and UBE2A) exhibited a higher expression in females than in parthenotes, suggesting that they are predominantly expressed by the paternal inherited X-Chr and that imprinting may increase the transcriptional skew caused by double X-Chr dosage.
Main Authors: | Bermejo-Alvarez, P., Rizos, D., Rath, D., Lonergan, P., Gutierrez-Adan, A. |
---|---|
Format: | journal article biblioteca |
Language: | eng |
Published: |
2010
|
Online Access: | http://hdl.handle.net/20.500.12792/3530 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Similar Items
-
Sex determines the expression level of one third of the actively expressed genes in bovine blastocysts
by: Bermejo Álvarez, Pablo, et al.
Published: (2010) -
Developmental kinetics and gene expression in male and female bovine embryos produced in vitro with sex-sorted spermatozoa
by: Bermejo-Álvarez, P., et al.
Published: (2010) -
Developmental kinetics and gene expression in male and female bovine embryos produced in vitro with sex-sorted spermatozoa
by: Bermejo Álvarez, Pablo, et al.
Published: (2010) -
Epigenetic differences between male and female bovine blastocysts produced in vitro
by: Bermejo-Álvarez, P., et al.
Published: (2008) -
Amino acid metabolism of bovine blastocysts A biomarker of sex and viability
by: Sturmey, R. G., et al.
Published: (2010)