Progesterone effects on mouse sperm kinetics in conditions of viscosity

The spermatozoa delivered to the female genital tract need to swim towards the oocyte through viscous secretions. Once close to the oocyte, the spermatozoa are guided by a gradient of progesterone (P4 ) and other unknown chemoattractants via a process known as chemotaxis. Using polyvinylpyrrolidone to establish the conditions of viscosity, we examined the response of mouse spermatozoa to P4 . Herein, we show that in low-viscous media, P4 induces hyperactive-like motility whereby sperm show erratic trajectories and non-progressive movement. However, an opposite response is produced in viscous medium in that trajectories are linear and motility is more progressive and less erratic. Our observations provide a behavioural explanation for the chemotaxis of spermatozoa swimming under viscous conditions in a spatial gradient of the chemoattractant P4 . They also highlight the importance of using viscous solutions to mimic in vivo conditions when analysing sperm behaviour in response to any stimulus. © 2016 Society for Reproduction and Fertility.

Bibliographic Details

Main Authors:	Pérez-Cerezales, S., López-Cardona, A. P., Gutiérrez-Adán, A.
Format:	journal article biblioteca
Language:	eng
Published:	2016
Online Access:	http://hdl.handle.net/20.500.12792/3432
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