Novel isolation of water-soluble polysaccharides from the fruiting bodies of Pleurotus ostreatus mushrooms
Novel water-soluble polysaccharides have been isolated from the fruiting bodies of the edible mushroom Pleurotus ostreatus. Three polysaccharide fractions were obtained by ethanol precipitation from cold water, hot water and hot aqueous NaOH extracts. The fractions were purified by size exclusion chromatography showing a unique carbohydrate occurring in each fraction PC from the cold fraction, PH from the hot fraction and PB from the hot aqueous NaOH fraction. The analysis of the methylated alditol acetates and the NMR studies revealed that all the polysaccharides displayed a linear backbone. PC was formed by α-(1→3),(1→6)-linked galactopyranosyl residues whereas PH and PB consisted of glucose-linked units. PH was exclusively composed of glucopyranosyl units bound by α-(1→4) linkages whereas PB was a β-linked glucan showing (1→3) and (1→6) glycosidic bonds. The analysis of molecular arrangement by complexation with Congo red showed that only the β-linked polysaccharide (PB) displayed a triple helix conformation. © 2012 Elsevier Ltd. All rights reserved.
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dig-inia-es-20.500.12792-32812020-12-15T09:52:39Z Novel isolation of water-soluble polysaccharides from the fruiting bodies of Pleurotus ostreatus mushrooms Palacios, I. García-Lafuente, A. Guillamón, E. Villares, A. Novel water-soluble polysaccharides have been isolated from the fruiting bodies of the edible mushroom Pleurotus ostreatus. Three polysaccharide fractions were obtained by ethanol precipitation from cold water, hot water and hot aqueous NaOH extracts. The fractions were purified by size exclusion chromatography showing a unique carbohydrate occurring in each fraction PC from the cold fraction, PH from the hot fraction and PB from the hot aqueous NaOH fraction. The analysis of the methylated alditol acetates and the NMR studies revealed that all the polysaccharides displayed a linear backbone. PC was formed by α-(1→3),(1→6)-linked galactopyranosyl residues whereas PH and PB consisted of glucose-linked units. PH was exclusively composed of glucopyranosyl units bound by α-(1→4) linkages whereas PB was a β-linked glucan showing (1→3) and (1→6) glycosidic bonds. The analysis of molecular arrangement by complexation with Congo red showed that only the β-linked polysaccharide (PB) displayed a triple helix conformation. © 2012 Elsevier Ltd. All rights reserved. 2020-10-22T14:22:14Z 2020-10-22T14:22:14Z 2012 journal article http://hdl.handle.net/20.500.12792/3281 10.1016/j.carres.2012.06.016 eng Attribution-NonCommercial-ShareAlike 4.0 International http://creativecommons.org/licenses/by-nc-sa/4.0/ open access |
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Novel water-soluble polysaccharides have been isolated from the fruiting bodies of the edible mushroom Pleurotus ostreatus. Three polysaccharide fractions were obtained by ethanol precipitation from cold water, hot water and hot aqueous NaOH extracts. The fractions were purified by size exclusion chromatography showing a unique carbohydrate occurring in each fraction PC from the cold fraction, PH from the hot fraction and PB from the hot aqueous NaOH fraction. The analysis of the methylated alditol acetates and the NMR studies revealed that all the polysaccharides displayed a linear backbone. PC was formed by α-(1→3),(1→6)-linked galactopyranosyl residues whereas PH and PB consisted of glucose-linked units. PH was exclusively composed of glucopyranosyl units bound by α-(1→4) linkages whereas PB was a β-linked glucan showing (1→3) and (1→6) glycosidic bonds. The analysis of molecular arrangement by complexation with Congo red showed that only the β-linked polysaccharide (PB) displayed a triple helix conformation. © 2012 Elsevier Ltd. All rights reserved. |
| format |
journal article |
| author |
Palacios, I. García-Lafuente, A. Guillamón, E. Villares, A. |
| spellingShingle |
Palacios, I. García-Lafuente, A. Guillamón, E. Villares, A. Novel isolation of water-soluble polysaccharides from the fruiting bodies of Pleurotus ostreatus mushrooms |
| author_facet |
Palacios, I. García-Lafuente, A. Guillamón, E. Villares, A. |
| author_sort |
Palacios, I. |
| title |
Novel isolation of water-soluble polysaccharides from the fruiting bodies of Pleurotus ostreatus mushrooms |
| title_short |
Novel isolation of water-soluble polysaccharides from the fruiting bodies of Pleurotus ostreatus mushrooms |
| title_full |
Novel isolation of water-soluble polysaccharides from the fruiting bodies of Pleurotus ostreatus mushrooms |
| title_fullStr |
Novel isolation of water-soluble polysaccharides from the fruiting bodies of Pleurotus ostreatus mushrooms |
| title_full_unstemmed |
Novel isolation of water-soluble polysaccharides from the fruiting bodies of Pleurotus ostreatus mushrooms |
| title_sort |
novel isolation of water-soluble polysaccharides from the fruiting bodies of pleurotus ostreatus mushrooms |
| publishDate |
2012 |
| url |
http://hdl.handle.net/20.500.12792/3281 |
| work_keys_str_mv |
AT palaciosi novelisolationofwatersolublepolysaccharidesfromthefruitingbodiesofpleurotusostreatusmushrooms AT garcialafuentea novelisolationofwatersolublepolysaccharidesfromthefruitingbodiesofpleurotusostreatusmushrooms AT guillamone novelisolationofwatersolublepolysaccharidesfromthefruitingbodiesofpleurotusostreatusmushrooms AT villaresa novelisolationofwatersolublepolysaccharidesfromthefruitingbodiesofpleurotusostreatusmushrooms |
| _version_ |
1758004894133911552 |