Absence of E protein arrests transmissible gastroenteritis coronavirus maturation in the secretory pathway
A recombinant transmissible gastroenteritis coronavirus (rTGEV) in which E gene was deleted (rTGEV-ΔE) has been engineered. This deletion mutant only grows in cells expressing E protein (E+ cells) indicating that E was an essential gene for TGEV replication. Electron microscopy studies of rTGEV-ΔE infected BHK-pAPN-E- cells showed that only immature intracellular virions were assembled. These virions were non-infectious and not secreted to the extracellular medium in BHK-pAPN-E- cells. RNA and protein composition analysis by RNase-gold and immunoelectron microscopy showed that rTGEV-ΔE virions contained RNA and also all the structural TGEV proteins, except the deleted E protein. Nevertheless, full virion maturation was blocked. Studies of the rTGEV-ΔE subcellular localization by confocal and immunoelectron microscopy in infected E- cells showed that in the absence of E protein virus trafficking was arrested in the intermediate compartment. Therefore, the absence of E protein in TGEV resulted in two actions, a blockade of virus trafficking in the membranes of the secretory pathway, and prevention of full virus maturation. © 2007 Elsevier Inc. All rights reserved.
Main Authors: | , , , , |
---|---|
Format: | journal article biblioteca |
Language: | eng |
Published: |
2007
|
Online Access: | http://hdl.handle.net/20.500.12792/3234 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
id |
dig-inia-es-20.500.12792-3234 |
---|---|
record_format |
koha |
spelling |
dig-inia-es-20.500.12792-32342020-12-15T09:54:26Z Absence of E protein arrests transmissible gastroenteritis coronavirus maturation in the secretory pathway Ortego, J. Ceriani, J. E. Patiño, C. Plana, J. Enjuanes, L. A recombinant transmissible gastroenteritis coronavirus (rTGEV) in which E gene was deleted (rTGEV-ΔE) has been engineered. This deletion mutant only grows in cells expressing E protein (E+ cells) indicating that E was an essential gene for TGEV replication. Electron microscopy studies of rTGEV-ΔE infected BHK-pAPN-E- cells showed that only immature intracellular virions were assembled. These virions were non-infectious and not secreted to the extracellular medium in BHK-pAPN-E- cells. RNA and protein composition analysis by RNase-gold and immunoelectron microscopy showed that rTGEV-ΔE virions contained RNA and also all the structural TGEV proteins, except the deleted E protein. Nevertheless, full virion maturation was blocked. Studies of the rTGEV-ΔE subcellular localization by confocal and immunoelectron microscopy in infected E- cells showed that in the absence of E protein virus trafficking was arrested in the intermediate compartment. Therefore, the absence of E protein in TGEV resulted in two actions, a blockade of virus trafficking in the membranes of the secretory pathway, and prevention of full virus maturation. © 2007 Elsevier Inc. All rights reserved. 2020-10-22T14:16:49Z 2020-10-22T14:16:49Z 2007 journal article http://hdl.handle.net/20.500.12792/3234 10.1016/j.virol.2007.05.032 eng Attribution-NonCommercial-ShareAlike 4.0 International http://creativecommons.org/licenses/by-nc-sa/4.0/ open access |
institution |
INIA ES |
collection |
DSpace |
country |
España |
countrycode |
ES |
component |
Bibliográfico |
access |
En linea |
databasecode |
dig-inia-es |
tag |
biblioteca |
region |
Europa del Sur |
libraryname |
Biblioteca del INIA España |
language |
eng |
description |
A recombinant transmissible gastroenteritis coronavirus (rTGEV) in which E gene was deleted (rTGEV-ΔE) has been engineered. This deletion mutant only grows in cells expressing E protein (E+ cells) indicating that E was an essential gene for TGEV replication. Electron microscopy studies of rTGEV-ΔE infected BHK-pAPN-E- cells showed that only immature intracellular virions were assembled. These virions were non-infectious and not secreted to the extracellular medium in BHK-pAPN-E- cells. RNA and protein composition analysis by RNase-gold and immunoelectron microscopy showed that rTGEV-ΔE virions contained RNA and also all the structural TGEV proteins, except the deleted E protein. Nevertheless, full virion maturation was blocked. Studies of the rTGEV-ΔE subcellular localization by confocal and immunoelectron microscopy in infected E- cells showed that in the absence of E protein virus trafficking was arrested in the intermediate compartment. Therefore, the absence of E protein in TGEV resulted in two actions, a blockade of virus trafficking in the membranes of the secretory pathway, and prevention of full virus maturation. © 2007 Elsevier Inc. All rights reserved. |
format |
journal article |
author |
Ortego, J. Ceriani, J. E. Patiño, C. Plana, J. Enjuanes, L. |
spellingShingle |
Ortego, J. Ceriani, J. E. Patiño, C. Plana, J. Enjuanes, L. Absence of E protein arrests transmissible gastroenteritis coronavirus maturation in the secretory pathway |
author_facet |
Ortego, J. Ceriani, J. E. Patiño, C. Plana, J. Enjuanes, L. |
author_sort |
Ortego, J. |
title |
Absence of E protein arrests transmissible gastroenteritis coronavirus maturation in the secretory pathway |
title_short |
Absence of E protein arrests transmissible gastroenteritis coronavirus maturation in the secretory pathway |
title_full |
Absence of E protein arrests transmissible gastroenteritis coronavirus maturation in the secretory pathway |
title_fullStr |
Absence of E protein arrests transmissible gastroenteritis coronavirus maturation in the secretory pathway |
title_full_unstemmed |
Absence of E protein arrests transmissible gastroenteritis coronavirus maturation in the secretory pathway |
title_sort |
absence of e protein arrests transmissible gastroenteritis coronavirus maturation in the secretory pathway |
publishDate |
2007 |
url |
http://hdl.handle.net/20.500.12792/3234 |
work_keys_str_mv |
AT ortegoj absenceofeproteinarreststransmissiblegastroenteritiscoronavirusmaturationinthesecretorypathway AT cerianije absenceofeproteinarreststransmissiblegastroenteritiscoronavirusmaturationinthesecretorypathway AT patinoc absenceofeproteinarreststransmissiblegastroenteritiscoronavirusmaturationinthesecretorypathway AT planaj absenceofeproteinarreststransmissiblegastroenteritiscoronavirusmaturationinthesecretorypathway AT enjuanesl absenceofeproteinarreststransmissiblegastroenteritiscoronavirusmaturationinthesecretorypathway |
_version_ |
1758004761132531712 |