Absence of E protein arrests transmissible gastroenteritis coronavirus maturation in the secretory pathway
A recombinant transmissible gastroenteritis coronavirus (rTGEV) in which E gene was deleted (rTGEV-ΔE) has been engineered. This deletion mutant only grows in cells expressing E protein (E+ cells) indicating that E was an essential gene for TGEV replication. Electron microscopy studies of rTGEV-ΔE infected BHK-pAPN-E- cells showed that only immature intracellular virions were assembled. These virions were non-infectious and not secreted to the extracellular medium in BHK-pAPN-E- cells. RNA and protein composition analysis by RNase-gold and immunoelectron microscopy showed that rTGEV-ΔE virions contained RNA and also all the structural TGEV proteins, except the deleted E protein. Nevertheless, full virion maturation was blocked. Studies of the rTGEV-ΔE subcellular localization by confocal and immunoelectron microscopy in infected E- cells showed that in the absence of E protein virus trafficking was arrested in the intermediate compartment. Therefore, the absence of E protein in TGEV resulted in two actions, a blockade of virus trafficking in the membranes of the secretory pathway, and prevention of full virus maturation. © 2007 Elsevier Inc. All rights reserved.
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dig-inia-es-20.500.12792-32342020-12-15T09:54:26Z Absence of E protein arrests transmissible gastroenteritis coronavirus maturation in the secretory pathway Ortego, J. Ceriani, J. E. Patiño, C. Plana, J. Enjuanes, L. A recombinant transmissible gastroenteritis coronavirus (rTGEV) in which E gene was deleted (rTGEV-ΔE) has been engineered. This deletion mutant only grows in cells expressing E protein (E+ cells) indicating that E was an essential gene for TGEV replication. Electron microscopy studies of rTGEV-ΔE infected BHK-pAPN-E- cells showed that only immature intracellular virions were assembled. These virions were non-infectious and not secreted to the extracellular medium in BHK-pAPN-E- cells. RNA and protein composition analysis by RNase-gold and immunoelectron microscopy showed that rTGEV-ΔE virions contained RNA and also all the structural TGEV proteins, except the deleted E protein. Nevertheless, full virion maturation was blocked. Studies of the rTGEV-ΔE subcellular localization by confocal and immunoelectron microscopy in infected E- cells showed that in the absence of E protein virus trafficking was arrested in the intermediate compartment. Therefore, the absence of E protein in TGEV resulted in two actions, a blockade of virus trafficking in the membranes of the secretory pathway, and prevention of full virus maturation. © 2007 Elsevier Inc. All rights reserved. 2020-10-22T14:16:49Z 2020-10-22T14:16:49Z 2007 journal article http://hdl.handle.net/20.500.12792/3234 10.1016/j.virol.2007.05.032 eng Attribution-NonCommercial-ShareAlike 4.0 International http://creativecommons.org/licenses/by-nc-sa/4.0/ open access |
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A recombinant transmissible gastroenteritis coronavirus (rTGEV) in which E gene was deleted (rTGEV-ΔE) has been engineered. This deletion mutant only grows in cells expressing E protein (E+ cells) indicating that E was an essential gene for TGEV replication. Electron microscopy studies of rTGEV-ΔE infected BHK-pAPN-E- cells showed that only immature intracellular virions were assembled. These virions were non-infectious and not secreted to the extracellular medium in BHK-pAPN-E- cells. RNA and protein composition analysis by RNase-gold and immunoelectron microscopy showed that rTGEV-ΔE virions contained RNA and also all the structural TGEV proteins, except the deleted E protein. Nevertheless, full virion maturation was blocked. Studies of the rTGEV-ΔE subcellular localization by confocal and immunoelectron microscopy in infected E- cells showed that in the absence of E protein virus trafficking was arrested in the intermediate compartment. Therefore, the absence of E protein in TGEV resulted in two actions, a blockade of virus trafficking in the membranes of the secretory pathway, and prevention of full virus maturation. © 2007 Elsevier Inc. All rights reserved. |
| format |
journal article |
| author |
Ortego, J. Ceriani, J. E. Patiño, C. Plana, J. Enjuanes, L. |
| spellingShingle |
Ortego, J. Ceriani, J. E. Patiño, C. Plana, J. Enjuanes, L. Absence of E protein arrests transmissible gastroenteritis coronavirus maturation in the secretory pathway |
| author_facet |
Ortego, J. Ceriani, J. E. Patiño, C. Plana, J. Enjuanes, L. |
| author_sort |
Ortego, J. |
| title |
Absence of E protein arrests transmissible gastroenteritis coronavirus maturation in the secretory pathway |
| title_short |
Absence of E protein arrests transmissible gastroenteritis coronavirus maturation in the secretory pathway |
| title_full |
Absence of E protein arrests transmissible gastroenteritis coronavirus maturation in the secretory pathway |
| title_fullStr |
Absence of E protein arrests transmissible gastroenteritis coronavirus maturation in the secretory pathway |
| title_full_unstemmed |
Absence of E protein arrests transmissible gastroenteritis coronavirus maturation in the secretory pathway |
| title_sort |
absence of e protein arrests transmissible gastroenteritis coronavirus maturation in the secretory pathway |
| publishDate |
2007 |
| url |
http://hdl.handle.net/20.500.12792/3234 |
| work_keys_str_mv |
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| _version_ |
1758004761132531712 |