Foodborne transmission of bovine spongiform encephalopathy to nonhuman primates

Risk for human exposure to bovine spongiform encephalopathy (BSE)-inducing agent was estimated in a nonhuman primate model. To determine attack rates, incubation times, and molecular signatures, we orally exposed 18 macaques to 1 high dose of brain material from cattle with BSE. Several macaques were euthanized at regular intervals starting at 1 year postinoculation, and others were observed until clinical signs developed. Among those who received >5 g BSE-inducing agent, attack rates were 100% and prions could be detected in peripheral tissues from 1 year postinoculation onward. The overall median incubation time was 4.6 years (3.7-5.3). However, for 3 macaques orally exposed on multiple occasions, incubation periods were at least 7-10 years. Before clinical signs were noted, we detected a non-type 2B signature, indicating the existence of atypical prion protein during the incubation period. This finding could affect diagnosis of variant Creutzfeldt-Jakob disease in humans and might be relevant for retrospective studies of positive tonsillectomy or appendectomy specimens because time of infection is unknown.

Bibliographic Details

Main Authors:	Holznagel, E., Yutzy, B., Schulz-Schaeffer, W., Kruip, C., Hahmann, U., Bierke, P., Torres, J. M., Kim, Y. S., Thomzig, A., Beekes, M., Hunsmann, G., Loewer, J.
Format:	journal article biblioteca
Language:	eng
Published:	2013
Online Access:	http://hdl.handle.net/20.500.12792/2123
Tags:	Add Tag No Tags, Be the first to tag this record!