A Novel Bacteriophage Infecting Multi-Drug- and Extended-Drug-Resistant Pseudomonas aeruginosa Strains
22 Pág.
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| Language: | English |
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Multidisciplinary Digital Publishing Institute
2024-06-03
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| Subjects: | Bacteriophages, Multidrug-resistance, Phage therapy, Pseudomonas aeruginosa, Treatment, |
| Online Access: | http://hdl.handle.net/10261/368875 http://dx.doi.org/10.13039/100012818 http://dx.doi.org/10.13039/501100004837 http://dx.doi.org/10.13039/501100004587 http://dx.doi.org/10.13039/501100000780 https://api.elsevier.com/content/abstract/scopus_id/85196900176 |
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dig-inia-es-10261-3688752024-11-04T21:59:20Z A Novel Bacteriophage Infecting Multi-Drug- and Extended-Drug-Resistant Pseudomonas aeruginosa Strains Santamaría-Corral, Guillermo Pagán, Israel Aguilera-Correa, John Jairo Esteban, Jaime García-Quintanilla, Meritxell Ministerio de Ciencia e Innovación (España) Instituto de Salud Carlos III European Commission Comunidad de Madrid Santamaría-Corral, Guillermo [0000-0002-7544-0604] Pagán, Israel [0000-0001-8876-1194] Aguilera-Correa, John Jairo [0000-0002-4002-1944] Esteban, Jaime [0000-0002-8971-3167] García-Quintanilla, Meritxell [0000-0003-1889-1377] Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72] Bacteriophages Multidrug-resistance Phage therapy Pseudomonas aeruginosa Treatment 22 Pág. The prevalence of carbapenem-resistant P. aeruginosa has dramatically increased over the last decade, and antibiotics alone are not enough to eradicate infections caused by this opportunistic pathogen. Phage therapy is a fresh treatment that can be administered under compassionate use, particularly against chronic cases. However, it is necessary to thoroughly characterize the virus before therapeutic application. Our work describes the discovery of the novel sequenced bacteriophage, vB_PaeP-F1Pa, containing an integrase, performs a phylogenetical analysis, describes its stability at a physiological pH and temperature, latent period (40 min), and burst size (394 ± 166 particles per bacterial cell), and demonstrates its ability to infect MDR and XDR P. aeruginosa strains. Moreover, this novel bacteriophage was able to inhibit the growth of bacteria inside preformed biofilms. The present study offers a road map to analyze essential areas for successful phage therapy against MDR and XDR P. aeruginosa infections, and shows that a phage containing an integrase is also able to show good in vitro results, indicating that it is very important to perform a genomic analysis before any clinical use, in order to prevent adverse effects in patients. This research received no specific grant from any funding agency in the public, commercial, or not-for-profit sectors. MG-Q is supported by the Subprograma Miguel Servet from the Ministerio de Ciencia e Innovación of Spain (CP19/00104), Instituto de Salud Carlos III (Plan Estatal de I+D+i 2017–2020), and co-funded by the European Social Fund “Investing in your future”. GS-C was supported by the Programa Operativo de Empleo Juvenil and Iniciativa de Empleo Juvenil (YEI) from the Consejería de Ciencia, Universidades e Innovación from Comunidad de Madrid and the European Union, Fondo Social Europeo: “El FSE invierte en tu futuro”. IP was supported by Ministerio de Ciencia e Innovación (Proyectos de Transición Ecológica y Digital, TED2021-130793B-I00). Peer reviewed 2024-10-02T06:49:19Z 2024-10-02T06:49:19Z 2024-06-03 artículo http://purl.org/coar/resource_type/c_6501 Publisher's version Antibiotics 13(6): 523 (2024) http://hdl.handle.net/10261/368875 10.3390/antibiotics13060523 2079-6382 http://dx.doi.org/10.13039/100012818 http://dx.doi.org/10.13039/501100004837 http://dx.doi.org/10.13039/501100004587 http://dx.doi.org/10.13039/501100000780 2-s2.0-85196900176 https://api.elsevier.com/content/abstract/scopus_id/85196900176 en #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/MICINN//TED2021-130793B-I00 Centro de Biotecnología y Genómica de Plantas (CBGP) https://doi.org/10.3390/antibiotics13060523 Sí open application/pdf Multidisciplinary Digital Publishing Institute |
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biblioteca |
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Bacteriophages Multidrug-resistance Phage therapy Pseudomonas aeruginosa Treatment Bacteriophages Multidrug-resistance Phage therapy Pseudomonas aeruginosa Treatment |
| spellingShingle |
Bacteriophages Multidrug-resistance Phage therapy Pseudomonas aeruginosa Treatment Bacteriophages Multidrug-resistance Phage therapy Pseudomonas aeruginosa Treatment Santamaría-Corral, Guillermo Pagán, Israel Aguilera-Correa, John Jairo Esteban, Jaime García-Quintanilla, Meritxell A Novel Bacteriophage Infecting Multi-Drug- and Extended-Drug-Resistant Pseudomonas aeruginosa Strains |
| description |
22 Pág. |
| author2 |
Ministerio de Ciencia e Innovación (España) |
| author_facet |
Ministerio de Ciencia e Innovación (España) Santamaría-Corral, Guillermo Pagán, Israel Aguilera-Correa, John Jairo Esteban, Jaime García-Quintanilla, Meritxell |
| format |
artículo |
| topic_facet |
Bacteriophages Multidrug-resistance Phage therapy Pseudomonas aeruginosa Treatment |
| author |
Santamaría-Corral, Guillermo Pagán, Israel Aguilera-Correa, John Jairo Esteban, Jaime García-Quintanilla, Meritxell |
| author_sort |
Santamaría-Corral, Guillermo |
| title |
A Novel Bacteriophage Infecting Multi-Drug- and Extended-Drug-Resistant Pseudomonas aeruginosa Strains |
| title_short |
A Novel Bacteriophage Infecting Multi-Drug- and Extended-Drug-Resistant Pseudomonas aeruginosa Strains |
| title_full |
A Novel Bacteriophage Infecting Multi-Drug- and Extended-Drug-Resistant Pseudomonas aeruginosa Strains |
| title_fullStr |
A Novel Bacteriophage Infecting Multi-Drug- and Extended-Drug-Resistant Pseudomonas aeruginosa Strains |
| title_full_unstemmed |
A Novel Bacteriophage Infecting Multi-Drug- and Extended-Drug-Resistant Pseudomonas aeruginosa Strains |
| title_sort |
novel bacteriophage infecting multi-drug- and extended-drug-resistant pseudomonas aeruginosa strains |
| publisher |
Multidisciplinary Digital Publishing Institute |
| publishDate |
2024-06-03 |
| url |
http://hdl.handle.net/10261/368875 http://dx.doi.org/10.13039/100012818 http://dx.doi.org/10.13039/501100004837 http://dx.doi.org/10.13039/501100004587 http://dx.doi.org/10.13039/501100000780 https://api.elsevier.com/content/abstract/scopus_id/85196900176 |
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