Influenza D virus utilizes both 9-O-acetylated Nacetylneuraminic and 9-O-acetylated N-glycolylneuraminic acids as functional entry receptors
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| Format: | artículo biblioteca |
| Language: | English |
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American Society for Microbiology
2024-02-20
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| Subjects: | 9Ac 2, CASD1, Influenza D, Neu5, Neu5Gc9Ac, Receptor, |
| Online Access: | http://hdl.handle.net/10261/360872 https://api.elsevier.com/content/abstract/scopus_id/85188479550 |
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dig-inia-es-10261-3608722024-10-28T21:39:07Z Influenza D virus utilizes both 9-O-acetylated Nacetylneuraminic and 9-O-acetylated N-glycolylneuraminic acids as functional entry receptors Uprety, Tirth Yu, Jieshi Nogales, Aitor Naveed, Ahsan Yu, Hai Chen, Xi Liu, Yunpeng Bowman, Andrew S. Martínez-Sobrido, Luis Parrish, Colin R. Melikyan, Gregory B. Wang, Dan Li, Feng University of Kentucky Uprety, Tirth [0000-0003-1945-1875] Yu, Jieshi [0000-0003-1466-1339] Nogales, Aitor [0000-0002-2424-7900] Bowman, Andrew S. [0000-0002-0738-8453] Martínez-Sobrido, Luis [0000-0001-7084-0804] Parrish, Colin R. [0000-0002-1836-6655] Li, Feng [0000-0002-8904-2893] Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72] 9Ac 2 CASD1 Influenza D Neu5 Neu5Gc9Ac Receptor 17 Pág. Influenza D virus (IDV) utilizes bovines as a primary reservoir with periodical spillover to other hosts. We have previously demonstrated that IDV binds both 9-O-acetylated N-acetylneuraminic acid (Neu5,9Ac2) and 9-O-acetylated N-glycolylneuraminic acid (Neu5Gc9Ac). Bovines produce both Neu5,9Ac2 and Neu5Gc9Ac, while humans are genetically unable to synthesize Neu5Gc9Ac. 9-O-Acetylation of sialic acids is catalyzed by CASD1 via a covalent acetyl-enzyme intermediate. To characterize the role of Neu5,9Ac2 and Neu5Gc9Ac in IDV infection and determine which form of 9-O-acetylated sialic acids drives IDV entry, we took advantage of a CASD1 knockout (KO) MDCK cell line and carried out feeding experiments using synthetic 9-O-acetyl sialic acids in combination with the single-round and multi-round IDV infection assays. The data from our studies show that (i) CASD1 KO cells are resistant to IDV infection and lack of IDV binding to the cell surface is responsible for the failure of IDV replication; (ii) feeding CASD1 KO cells with Neu5,9Ac2 or Neu5Gc9Ac resulted in a dose-dependent rescue of IDV infectivity; and (iii) diverse IDVs replicated robustly in CASD1 KO cells fed with either Neu5,9Ac2 or Neu5Gc9Ac at a level similar to that in wild-type cells with a functional CASD1. These data demonstrate that IDV can utilize Neu5,9Ac2- or non-human Neu5Gc9Ac-containing glycan receptor for infection. Our findings provide evidence that IDV has acquired the ability to infect and transmit among agricultural animals that are enriched in Neu5Gc9Ac, in addition to posing a zoonotic risk to humans expressing only Neu5,9Ac2 This study was supported by NIH R01AI141889. The authors also thank the support provided by the William Robert Mills Chair Endowment Fund and Agricultural Experiment Station of the University of Kentucky Peer reviewed 2024-06-19T08:08:16Z 2024-06-19T08:08:16Z 2024-02-20 artículo http://purl.org/coar/resource_type/c_6501 Journal of Virology 98(3): e0004224 (2024) 0022-538X http://hdl.handle.net/10261/360872 10.1128/jvi.00042-24 1098-5514 38376198 2-s2.0-85188479550 https://api.elsevier.com/content/abstract/scopus_id/85188479550 en Centro de Investigación en Sanidad Animal (CISA) https://doi.org/10.1128/jvi.00042-24 Sí open American Society for Microbiology |
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9Ac 2 CASD1 Influenza D Neu5 Neu5Gc9Ac Receptor 9Ac 2 CASD1 Influenza D Neu5 Neu5Gc9Ac Receptor |
| spellingShingle |
9Ac 2 CASD1 Influenza D Neu5 Neu5Gc9Ac Receptor 9Ac 2 CASD1 Influenza D Neu5 Neu5Gc9Ac Receptor Uprety, Tirth Yu, Jieshi Nogales, Aitor Naveed, Ahsan Yu, Hai Chen, Xi Liu, Yunpeng Bowman, Andrew S. Martínez-Sobrido, Luis Parrish, Colin R. Melikyan, Gregory B. Wang, Dan Li, Feng Influenza D virus utilizes both 9-O-acetylated Nacetylneuraminic and 9-O-acetylated N-glycolylneuraminic acids as functional entry receptors |
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17 Pág. |
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University of Kentucky |
| author_facet |
University of Kentucky Uprety, Tirth Yu, Jieshi Nogales, Aitor Naveed, Ahsan Yu, Hai Chen, Xi Liu, Yunpeng Bowman, Andrew S. Martínez-Sobrido, Luis Parrish, Colin R. Melikyan, Gregory B. Wang, Dan Li, Feng |
| format |
artículo |
| topic_facet |
9Ac 2 CASD1 Influenza D Neu5 Neu5Gc9Ac Receptor |
| author |
Uprety, Tirth Yu, Jieshi Nogales, Aitor Naveed, Ahsan Yu, Hai Chen, Xi Liu, Yunpeng Bowman, Andrew S. Martínez-Sobrido, Luis Parrish, Colin R. Melikyan, Gregory B. Wang, Dan Li, Feng |
| author_sort |
Uprety, Tirth |
| title |
Influenza D virus utilizes both 9-O-acetylated Nacetylneuraminic and 9-O-acetylated N-glycolylneuraminic acids as functional entry receptors |
| title_short |
Influenza D virus utilizes both 9-O-acetylated Nacetylneuraminic and 9-O-acetylated N-glycolylneuraminic acids as functional entry receptors |
| title_full |
Influenza D virus utilizes both 9-O-acetylated Nacetylneuraminic and 9-O-acetylated N-glycolylneuraminic acids as functional entry receptors |
| title_fullStr |
Influenza D virus utilizes both 9-O-acetylated Nacetylneuraminic and 9-O-acetylated N-glycolylneuraminic acids as functional entry receptors |
| title_full_unstemmed |
Influenza D virus utilizes both 9-O-acetylated Nacetylneuraminic and 9-O-acetylated N-glycolylneuraminic acids as functional entry receptors |
| title_sort |
influenza d virus utilizes both 9-o-acetylated nacetylneuraminic and 9-o-acetylated n-glycolylneuraminic acids as functional entry receptors |
| publisher |
American Society for Microbiology |
| publishDate |
2024-02-20 |
| url |
http://hdl.handle.net/10261/360872 https://api.elsevier.com/content/abstract/scopus_id/85188479550 |
| work_keys_str_mv |
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1816136673986084864 |