Complex control of seed germination timing by ERF50 involves RGL2 antagonism and negative feedback regulation of DOG1

Complex control of seed germination timing by ERF50 involves RGL2 antagonism and negative feedback regulation of DOG1

17 Pág.

Saved in:
Bibliographic Details
Main Authors: Carrera-Castaño, Gerardo, Mira, Sara, Fañanás-Pueyo, Iris, Sánchez, Rocío, Contreras, Ángela, Weiste, Christoph, Dröge-Laser, Wolfgang, Plaza Gómez, Luis Manuel, Oñate-Sánchez, Luis
Other Authors: Agencia Estatal de Investigación (España)
Format: artículo biblioteca
Language:English
Published: John Wiley & Sons 2024-06
Subjects:Arabidopsis, DELLA, DOG1, ERF50/DREB2G, EXPA2, Seed dormancy, Seed germination,
Online Access:http://hdl.handle.net/10261/358115
https://api.elsevier.com/content/abstract/scopus_id/85188434489
Tags: Add Tag
No Tags, Be the first to tag this record!
id dig-inia-es-10261-358115
record_format koha
spelling dig-inia-es-10261-3581152024-05-23T20:38:17Z Complex control of seed germination timing by ERF50 involves RGL2 antagonism and negative feedback regulation of DOG1 Carrera-Castaño, Gerardo Mira, Sara Fañanás-Pueyo, Iris Sánchez, Rocío Contreras, Ángela Weiste, Christoph Dröge-Laser, Wolfgang Plaza Gómez, Luis Manuel Oñate-Sánchez, Luis Agencia Estatal de Investigación (España) Ministerio de Ciencia e Innovación (España) European Commission Universidad Politécnica de Madrid Comunidad de Madrid Carrera-Castaño, Gerardo [0000-0002-0393-3186] Mira, Sara [0000-0002-0164-5156] Fañanás-Pueyo, Iris [0000-0001-7608-1538] Sánchez, Rocío [0009-0000-5805-3496] Contreras, Ángela [0000-0003-3780-8428] Weiste, Christoph [0000-0003-1894-7924] Dröge-Laser, Wolfgang [0000-0003-4066-8024] Plaza Gómez, Luis Manuel [0000-0002-4346-4820] Oñate-Sánchez, Luis [0000-0001-7047-5739] Arabidopsis DELLA DOG1 ERF50/DREB2G EXPA2 Seed dormancy Seed germination 17 Pág. Seed dormancy governs germination timing, with both evolutionary and applied consequences. Despite extensive studies on the hormonal and genetic control of these processes, molecular mechanisms directly linking dormancy and germination remain poorly understood. By screening a collection of lines overexpressing Arabidopsis transcription factors, we identified ERF50 as a key gene to control dormancy and germination. To study its regulation, we measured seed-related physiological parameters in loss-of-function mutants and carried out transactivation, protein interaction and ChIP-PCR analyses. We found direct ERF50-mediated repression of DOG1 and activation of EXPA2 transcription, which results in enhanced seed germination. Although ERF50 expression is increased by DOG1 in dormant seeds, ERF50 germination-promoting activity is blocked by RGL2. The physiological, genetic and molecular evidence gathered here supports that ERF50 controls germination timing by regulating DOG1 levels to leverage its role as enhancer of seed germination, via RGL2 antagonism on EXPA2 expression. Our results highlight the central role of ERF50 as a feedback regulator to couple and fine-tune seed dormancy and germination. This work was supported with grants PID2019-109154RB-I00 and PID2022-142059OB-I00 funded by Spanish MCIN/AEI/10.13039/501100011033 and MCIN/AEI/10.13039/501100011033/FEDER, UE, respectively. GC-C was supported by a PhD contract from Universidad Politécnica de Madrid (UPM; programa propio), IF-P is supported by a PhD contract (FPI) funded by grant SEV-2016-0672 to the CBGP (Centre of Excellence Severo Ochoa Program of the Agencia Estatal de Investigación, Spain) and RS-M had a contract associated to grant BIO2016-77840-R awarded to LO-S. We thank Drs. D. Alabadí, T.-p. Sun and Y. Xiang as well as NASC for sending mutant seeds, Drs. Holdsworth and Prat for providing resources for the experimental work and D. Fraga (funded by Comunidad de Madrid, PEJ-2020-TL/BIO-18153) for technical assistance. Peer reviewed 2024-05-23T08:13:48Z 2024-05-23T08:13:48Z 2024-06 artículo New Phytologist 242(5): 2026-2042 (2024) 0028-646X http://hdl.handle.net/10261/358115 10.1111/nph.19681 1469-8137 38494681 2-s2.0-85188434489 https://api.elsevier.com/content/abstract/scopus_id/85188434489 en #PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-109154RB-I00/ES/UTILIZACION DE COMPONENTES DE LA RUTA DE LAS GIBERELINAS PARA MEJORAR EL VIGOR DE LAS SEMILLAS Y CRECIMIENTO DE LOS CULTIVOS/ info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PID2022-142059OB-I00/ES/EXPLORANDO LAS INTERACCIONES ASOCIADAS A UN NUEVO MECANISMO DE DEGRADACION DE DELLAS: UNA NUEVA ESTRATEGIA PARA MEJORAR EL DESBALANCE NUTRICIONAL EN CULTIVOS/ S2020-TL/BIO-18153 Centro de Biotecnología y Genómica de Plantas (CBGP) Publisher's version https://doi.org/10.1111/nph.19681 Sí open application/pdf John Wiley & Sons
institution INIA ES
collection DSpace
country España
countrycode ES
component Bibliográfico
access En linea
databasecode dig-inia-es
tag biblioteca
region Europa del Sur
libraryname Biblioteca del INIA España
language English
topic Arabidopsis
DELLA
DOG1
ERF50/DREB2G
EXPA2
Seed dormancy
Seed germination
Arabidopsis
DELLA
DOG1
ERF50/DREB2G
EXPA2
Seed dormancy
Seed germination
spellingShingle Arabidopsis
DELLA
DOG1
ERF50/DREB2G
EXPA2
Seed dormancy
Seed germination
Arabidopsis
DELLA
DOG1
ERF50/DREB2G
EXPA2
Seed dormancy
Seed germination
Carrera-Castaño, Gerardo
Mira, Sara
Fañanás-Pueyo, Iris
Sánchez, Rocío
Contreras, Ángela
Weiste, Christoph
Dröge-Laser, Wolfgang
Plaza Gómez, Luis Manuel
Oñate-Sánchez, Luis
Complex control of seed germination timing by ERF50 involves RGL2 antagonism and negative feedback regulation of DOG1
description 17 Pág.
author2 Agencia Estatal de Investigación (España)
author_facet Agencia Estatal de Investigación (España)
Carrera-Castaño, Gerardo
Mira, Sara
Fañanás-Pueyo, Iris
Sánchez, Rocío
Contreras, Ángela
Weiste, Christoph
Dröge-Laser, Wolfgang
Plaza Gómez, Luis Manuel
Oñate-Sánchez, Luis
format artículo
topic_facet Arabidopsis
DELLA
DOG1
ERF50/DREB2G
EXPA2
Seed dormancy
Seed germination
author Carrera-Castaño, Gerardo
Mira, Sara
Fañanás-Pueyo, Iris
Sánchez, Rocío
Contreras, Ángela
Weiste, Christoph
Dröge-Laser, Wolfgang
Plaza Gómez, Luis Manuel
Oñate-Sánchez, Luis
author_sort Carrera-Castaño, Gerardo
title Complex control of seed germination timing by ERF50 involves RGL2 antagonism and negative feedback regulation of DOG1
title_short Complex control of seed germination timing by ERF50 involves RGL2 antagonism and negative feedback regulation of DOG1
title_full Complex control of seed germination timing by ERF50 involves RGL2 antagonism and negative feedback regulation of DOG1
title_fullStr Complex control of seed germination timing by ERF50 involves RGL2 antagonism and negative feedback regulation of DOG1
title_full_unstemmed Complex control of seed germination timing by ERF50 involves RGL2 antagonism and negative feedback regulation of DOG1
title_sort complex control of seed germination timing by erf50 involves rgl2 antagonism and negative feedback regulation of dog1
publisher John Wiley & Sons
publishDate 2024-06
url http://hdl.handle.net/10261/358115
https://api.elsevier.com/content/abstract/scopus_id/85188434489
work_keys_str_mv AT carreracastanogerardo complexcontrolofseedgerminationtimingbyerf50involvesrgl2antagonismandnegativefeedbackregulationofdog1
AT mirasara complexcontrolofseedgerminationtimingbyerf50involvesrgl2antagonismandnegativefeedbackregulationofdog1
AT fananaspueyoiris complexcontrolofseedgerminationtimingbyerf50involvesrgl2antagonismandnegativefeedbackregulationofdog1
AT sanchezrocio complexcontrolofseedgerminationtimingbyerf50involvesrgl2antagonismandnegativefeedbackregulationofdog1
AT contrerasangela complexcontrolofseedgerminationtimingbyerf50involvesrgl2antagonismandnegativefeedbackregulationofdog1
AT weistechristoph complexcontrolofseedgerminationtimingbyerf50involvesrgl2antagonismandnegativefeedbackregulationofdog1
AT drogelaserwolfgang complexcontrolofseedgerminationtimingbyerf50involvesrgl2antagonismandnegativefeedbackregulationofdog1
AT plazagomezluismanuel complexcontrolofseedgerminationtimingbyerf50involvesrgl2antagonismandnegativefeedbackregulationofdog1
AT onatesanchezluis complexcontrolofseedgerminationtimingbyerf50involvesrgl2antagonismandnegativefeedbackregulationofdog1
_version_ 1802819945965289472

Similar Items