Nobiletin as a novel agent to enhance porcine in vitro embryo development and quality
11 Pág.
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| Format: | artículo biblioteca |
| Language: | English |
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Elsevier
2024-07-15
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| Subjects: | Antioxidant, Embryo, In vitro, Nobiletin, Pig, ROS, |
| Online Access: | http://hdl.handle.net/10261/356474 http://dx.doi.org/10.13039/501100004837 http://dx.doi.org/10.13039/501100011033 https://api.elsevier.com/content/abstract/scopus_id/85191163582 |
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Antioxidant Embryo In vitro Nobiletin Pig ROS Antioxidant Embryo In vitro Nobiletin Pig ROS |
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Antioxidant Embryo In vitro Nobiletin Pig ROS Antioxidant Embryo In vitro Nobiletin Pig ROS Cajas, Yulia N. Cañón-Beltrán, Karina Mazzarella, Rosane Núñez, Carolina González, Encina M. Rodriguez-Martinez, Heriberto Rizos, Dimitrios Martinez, Cristina A. Nobiletin as a novel agent to enhance porcine in vitro embryo development and quality |
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11 Pág. |
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Agencia Estatal de Investigación (España) |
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Agencia Estatal de Investigación (España) Cajas, Yulia N. Cañón-Beltrán, Karina Mazzarella, Rosane Núñez, Carolina González, Encina M. Rodriguez-Martinez, Heriberto Rizos, Dimitrios Martinez, Cristina A. |
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artículo |
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Antioxidant Embryo In vitro Nobiletin Pig ROS |
| author |
Cajas, Yulia N. Cañón-Beltrán, Karina Mazzarella, Rosane Núñez, Carolina González, Encina M. Rodriguez-Martinez, Heriberto Rizos, Dimitrios Martinez, Cristina A. |
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Cajas, Yulia N. |
| title |
Nobiletin as a novel agent to enhance porcine in vitro embryo development and quality |
| title_short |
Nobiletin as a novel agent to enhance porcine in vitro embryo development and quality |
| title_full |
Nobiletin as a novel agent to enhance porcine in vitro embryo development and quality |
| title_fullStr |
Nobiletin as a novel agent to enhance porcine in vitro embryo development and quality |
| title_full_unstemmed |
Nobiletin as a novel agent to enhance porcine in vitro embryo development and quality |
| title_sort |
nobiletin as a novel agent to enhance porcine in vitro embryo development and quality |
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Elsevier |
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2024-07-15 |
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http://hdl.handle.net/10261/356474 http://dx.doi.org/10.13039/501100004837 http://dx.doi.org/10.13039/501100011033 https://api.elsevier.com/content/abstract/scopus_id/85191163582 |
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1816136644089085952 |
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dig-inia-es-10261-3564742024-10-29T21:36:05Z Nobiletin as a novel agent to enhance porcine in vitro embryo development and quality Cajas, Yulia N. Cañón-Beltrán, Karina Mazzarella, Rosane Núñez, Carolina González, Encina M. Rodriguez-Martinez, Heriberto Rizos, Dimitrios Martinez, Cristina A. Agencia Estatal de Investigación (España) Ministerio de Ciencia e Innovación (España) Swedish Research Council Cajas, Yulia N. [0000-0001-9791-6733] Cañón-Beltrán, Karina [0000-0002-0279-0857] Mazzarella, Rosane [0000-0001-6860-8897] Núñez, Carolina [0000-0002-8020-6513] González, Encina M. [0000-0002-6217-866X] Rodriguez-Martinez, Heriberto [0000-0002-5194-2124] Rizos, Dimitrios [0000-0001-6813-3940] Martinez, Cristina A. [0000-0001-6811-0191] Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72] Antioxidant Embryo In vitro Nobiletin Pig ROS 11 Pág. In vitro embryo production (IVP) is of great importance to the porcine industry, as well as for basic research and biomedical applications. Despite the large efforts made in laboratories worldwide to address suboptimal culture conditions, porcine IVP remains inefficient. Nobiletin (Nob, 5,6,7,8,3',4' hexamethoxyflavone) supplementation to in vitro culture (IVC) medium, enhances in vitro embryo development in various species. However, its impact on the quality and developmental capacity of in vitro-produced pig embryos is yet to be established. This study evaluated the effects of different concentrations (2.5 and 5 μM) of Nob during the early culture of in vitro-produced pig embryos on embryo developmental competence, mitochondrial activity, lipid content, intracellular Reactive Oxygen Species (ROS) and Glutathione (GSH) content, Total Cell Number (TCN) per blastocyst, and expression of genes related to embryo development, quality and oxidative stress. Embryos cultured in medium without Nob supplementation and in medium supplemented with 0.01 % dimethyl sulfoxide (DMSO-vehicle for Nob) constituted the Control and DMSO groups, respectively. Embryo development rates were evaluated on Days 2, 6 and 7 of IVC. Additionally, a representative group of embryos was selected to assess mitochondrial activity, lipid, ROS and GSH content (on Days 2 and 6 of IVC), TCN assessment and gene expression analyses (on Day 6 of IVC). No significant differences were observed in any of the parameters evaluated on Day 2 of IVC. In contrast, embryos cultured under the presence of Nob 2.5 showed higher developmental rates on Days 6 and 7 of IVC. In addition, Day 6 embryos showed increased mitochondrial activity, with decreased levels of ROS and GSH in the Nob 2.5 group compared to the other groups. Both Nob 2.5 and Nob 5 embryos showed higher TCN compared to the Control and DMSO groups. Furthermore, Nob 2.5 and Nob 5 upregulated the expression of Superoxide dismutase type 1 (SOD1) and Glucose-6-phosphate dehydrogenase (G6PDH) genes, which could help to counteract oxidative stress during IVC. In conclusion, the addition of Nob during the first 48 h of IVC increased porcine embryo development rates and enhanced their quality, including the upregulation of relevant genes that potentially improved the overall efficiency of the IVP system. This work was supported by research project PID2019-111641RB-I00 (MCIN/AEI/10.13039/501100011033/to D.R.) and Swedish Research Council FORMAS (Project 2019-00288 to H.R.-M.). Peer reviewed 2024-05-09T10:54:36Z 2024-05-09T10:54:36Z 2024-07-15 artículo http://purl.org/coar/resource_type/c_6501 Theriogenology 223: 36-46 (2024) 0093-691X http://hdl.handle.net/10261/356474 10.1016/j.theriogenology.2024.04.011 1879-3231 http://dx.doi.org/10.13039/501100004837 http://dx.doi.org/10.13039/501100011033 38669840 2-s2.0-85191163582 https://api.elsevier.com/content/abstract/scopus_id/85191163582 en #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-111641RB-I00/ES/VESICULAS EXTRACELULARES: MODULADORES DE LA INTERACCION MATERNO-EMBRIONARIA Y DE LA FERTILIDAD EN BOVINO/ Departamento de Reproducción Animal Publisher's version https://doi.org/10.1016/j.theriogenology.2024.04.011 Sí open application/pdf Elsevier |