Effect of temperature and cell concentration on dolphin (Tursiops truncatus) spermatozoa quality evaluated at different days of refrigeration
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| Language: | English |
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Elsevier
2020-01
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| Subjects: | Cetacean, Conservation, Marine-animals, Sperm cells, |
| Online Access: | http://hdl.handle.net/10261/347007 https://api.elsevier.com/content/abstract/scopus_id/85076042839 |
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dig-inia-es-10261-3470072024-05-16T20:41:55Z Effect of temperature and cell concentration on dolphin (Tursiops truncatus) spermatozoa quality evaluated at different days of refrigeration Ruiz-Díaz, Sara Luongo, Chiara Fuentes-Albero, María Del Carmen Abril-Sánchez, S. Sánchez-Calabuig, M. J. Barros-García, Carlos de la Fé, Christian García-Galán, Ana Ros-Santaella, José Luis Pintus, Eliana García-Parraga, Daniel García-Vázquez, Francisco Alberto Ruiz-Díaz, Sara [0000-0002-3864-0156] Luongo, Chiara [0000-0003-0812-7033] Fuentes-Albero, María Del Carmen [0000-0002-2612-674X] Abril-Sánchez, S. [0000-0003-0963-6809] Sánchez-Calabuig, M. J. [0000-0002-4280-992X] de la Fé, Christian [0000-0002-4354-1689] García-Galán, Ana [0000-0001-5613-2975] Ros-Santaella, José Luis [0000-0002-4186-7730] Pintus, Eliana [0000-0002-8496-0820] García-Parraga, Daniel [0000-0002-3335-5831] García-Vázquez, Francisco Alberto [0000-0001-7665-3858] Cetacean Conservation Marine-animals Sperm cells 12 Pág. The use of cryopreserved dolphin spermatozoa facilitates the exchange of genetic material between aquatic parks and makes spermatozoa accessible to laboratories for studies to further our understanding of marine mammal reproduction. Sperm cryopreservation in the bottlenose dolphin (Tursiops truncatus) has been developed for the exchange of gametes within the ex situ population. The aim of this study was to develop an effective method for refrigeration of bottlenose dolphin spermatozoa diluted in a commercial extender (BTS). In Experiment 1, the effect of temperature (5 compared with 15 °C) on sperm quality was evaluated during 7 days of storage at 100 × 106 spermatozoa/ml. In Experiment 2, the effect of the storage concentration (100 × 106 compared with 20 × 106 spermatozoa/ml) on sperm quality was assessed during 7 days of storage at 5 °C. In Experiment 1, total motility (including % of rapid sperm) was greater at 5 than 15 °C. When the effect of storage concentration was evaluated (Experiment 2), total motility and ALH were greater at the higher storage concentration (100 × 106 spermatozoa/ml). For both experiments, values for viability, acrosome integrity, and normal morphology variables were consistent throughout the 7 days of refrigeration. In Experiment 3, a microbiological study was performed to evaluate the effect of the refrigeration temperature and days of storage on bacterial growth. The results of microbiological analysis indicated there was Staphylococcus aureus in some samples, however, there was no effect of temperature or days of refrigeration. In conclusion, bottlenose dolphin semen can be refrigerated for a short to medium period of storage and there is maintenance of functionality of sperm when stored at 100 × 106 spermatozoa/ml at 5 °C. There was not any specific grant from funding agencies in the public, commercial, or not-for-profit sectors obtained to conduct the research reported in this manuscript. Peer reviewed 2024-02-14T08:58:00Z 2024-02-14T08:58:00Z 2020-01 artículo http://purl.org/coar/resource_type/c_6501 Animal Reproduction Science 212: e106248 (2020) 0378-4320 http://hdl.handle.net/10261/347007 10.1016/j.anireprosci.2019.106248 31864493 2-s2.0-85076042839 https://api.elsevier.com/content/abstract/scopus_id/85076042839 en Departamento de Reproducción Animal Publisher's version https://doi.org/10.1016/j.anireprosci.2019.106248 Sí open Elsevier |
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Cetacean Conservation Marine-animals Sperm cells Cetacean Conservation Marine-animals Sperm cells |
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Cetacean Conservation Marine-animals Sperm cells Cetacean Conservation Marine-animals Sperm cells Ruiz-Díaz, Sara Luongo, Chiara Fuentes-Albero, María Del Carmen Abril-Sánchez, S. Sánchez-Calabuig, M. J. Barros-García, Carlos de la Fé, Christian García-Galán, Ana Ros-Santaella, José Luis Pintus, Eliana García-Parraga, Daniel García-Vázquez, Francisco Alberto Effect of temperature and cell concentration on dolphin (Tursiops truncatus) spermatozoa quality evaluated at different days of refrigeration |
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12 Pág. |
| author2 |
Ruiz-Díaz, Sara [0000-0002-3864-0156] |
| author_facet |
Ruiz-Díaz, Sara [0000-0002-3864-0156] Ruiz-Díaz, Sara Luongo, Chiara Fuentes-Albero, María Del Carmen Abril-Sánchez, S. Sánchez-Calabuig, M. J. Barros-García, Carlos de la Fé, Christian García-Galán, Ana Ros-Santaella, José Luis Pintus, Eliana García-Parraga, Daniel García-Vázquez, Francisco Alberto |
| format |
artículo |
| topic_facet |
Cetacean Conservation Marine-animals Sperm cells |
| author |
Ruiz-Díaz, Sara Luongo, Chiara Fuentes-Albero, María Del Carmen Abril-Sánchez, S. Sánchez-Calabuig, M. J. Barros-García, Carlos de la Fé, Christian García-Galán, Ana Ros-Santaella, José Luis Pintus, Eliana García-Parraga, Daniel García-Vázquez, Francisco Alberto |
| author_sort |
Ruiz-Díaz, Sara |
| title |
Effect of temperature and cell concentration on dolphin (Tursiops truncatus) spermatozoa quality evaluated at different days of refrigeration |
| title_short |
Effect of temperature and cell concentration on dolphin (Tursiops truncatus) spermatozoa quality evaluated at different days of refrigeration |
| title_full |
Effect of temperature and cell concentration on dolphin (Tursiops truncatus) spermatozoa quality evaluated at different days of refrigeration |
| title_fullStr |
Effect of temperature and cell concentration on dolphin (Tursiops truncatus) spermatozoa quality evaluated at different days of refrigeration |
| title_full_unstemmed |
Effect of temperature and cell concentration on dolphin (Tursiops truncatus) spermatozoa quality evaluated at different days of refrigeration |
| title_sort |
effect of temperature and cell concentration on dolphin (tursiops truncatus) spermatozoa quality evaluated at different days of refrigeration |
| publisher |
Elsevier |
| publishDate |
2020-01 |
| url |
http://hdl.handle.net/10261/347007 https://api.elsevier.com/content/abstract/scopus_id/85076042839 |
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