Nicotiana benthamiana resistance to non-adapted Melon necrotic spot virus results from an incompatible interaction between virus RNA and translation initiation factor 4E
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| Format: | artículo biblioteca |
| Language: | English |
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Wiley-Blackwell
2011-01-22
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| Subjects: | cap-independent translational enhancer, Melon necrotic spot virus, Nicotiana benthamiana, Non-host resistance, Susceptibility factor, Translation initiation factor eIF4E, |
| Online Access: | http://hdl.handle.net/10261/345157 http://dx.doi.org/10.13039/501100004837 http://dx.doi.org/10.13039/100007801 https://api.elsevier.com/content/abstract/scopus_id/79955383643 |
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dig-inia-es-10261-3451572024-05-17T20:36:02Z Nicotiana benthamiana resistance to non-adapted Melon necrotic spot virus results from an incompatible interaction between virus RNA and translation initiation factor 4E Nieto, Cristina Rodríguez-Moreno, Luis Rodríguez-Hernández, Ana M. Aranda, Miguel A. Truniger, Verónica Ministerio de Ciencia e Innovación (España) Fundación Séneca Nieto, Cristina [0000-0001-7226-314X] Rodríguez-Moreno, Luis [0000-0003-2385-8782] Truniger, Verónica [0000-0001-8221-2434] cap-independent translational enhancer Melon necrotic spot virus Nicotiana benthamiana Non-host resistance Susceptibility factor Translation initiation factor eIF4E 10 Pág. Nicotiana benthamiana has been described as non-host for Melon necrotic spot virus (MNSV). We investigated the basis of this resistance using the unique opportunity provided by strain MNSV-264, a recombinant virus that is able to overcome the resistance. Analysis of chimeric MNSV mutants showed that virulence in N. benthamiana is conferred by a 49 nucleotide section of the MNSV-264 3'-UTR, which acts in this host as a cap-independent translational enhancer (3'-CITE). Although the 3'-CITE of non-adapted MNSV-Mα5 is active in susceptible melon, it does not promote efficient translation in N. benthamiana, thus preventing expression of proteins required for virus replication. However, MNSV-Mα5 gains the ability to multiply in N. benthamiana cells if eIF4E from a susceptible melon variety (Cm-eIF4E-S) is supplied in trans. These data show that N. benthamiana resistance to MNSV-Mα5 results from incompatibility between the MNSV-Mα5 3'-CITE and N. benthamiana eIF4E in initiating efficient translation of the viral genome. Therefore, non-host resistance conferred by the inability of a host susceptibility factor to support viral multiplication may be a possible mechanism for this type of resistance to viruses. This work was supported by grants AGL2009-07552/AGR (Ministerio de Ciencia e Innovación, Spain) and EUI2009-04009 (PLANT-KBBE2009 program, Ministerio de Ciencia e Innovación, Spain) and 05702/PI/07 Fundación Séneca (Región de Murcia, Spain). Peer reviewed 2024-02-02T17:45:14Z 2024-02-02T17:45:14Z 2011-01-22 artículo http://purl.org/coar/resource_type/c_6501 Plant Journal 66(3): 492-501 (2011) 0960-7412 http://hdl.handle.net/10261/345157 10.1111/j.1365-313X.2011.04507.x 1365-313X http://dx.doi.org/10.13039/501100004837 http://dx.doi.org/10.13039/100007801 21255163 2-s2.0-79955383643 https://api.elsevier.com/content/abstract/scopus_id/79955383643 en #PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/MICINN//AGL2009-07552/ES/Traduccion De Rnas De Virus De Plantas Independiente De Cap: Analisis Del Modelo Experimental Mnsv%2FMelon Y Aplicaciones Biotecnologicas/ info:eu-repo/grantAgreement/MICINN//EUI2009-04009/ES/IDENTIFICATION OF NEW PLANT SUSCEPTIBILITY FACTORS WHOSE MODIFICATION WOULD CONFER VIRUS RESISTANCE/ The Plant journal : for cell and molecular biology Publisher's version https://doi.org/10.1111/j.1365-313X.2011.04507.x Sí open application/pdf Wiley-Blackwell |
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cap-independent translational enhancer Melon necrotic spot virus Nicotiana benthamiana Non-host resistance Susceptibility factor Translation initiation factor eIF4E cap-independent translational enhancer Melon necrotic spot virus Nicotiana benthamiana Non-host resistance Susceptibility factor Translation initiation factor eIF4E |
| spellingShingle |
cap-independent translational enhancer Melon necrotic spot virus Nicotiana benthamiana Non-host resistance Susceptibility factor Translation initiation factor eIF4E cap-independent translational enhancer Melon necrotic spot virus Nicotiana benthamiana Non-host resistance Susceptibility factor Translation initiation factor eIF4E Nieto, Cristina Rodríguez-Moreno, Luis Rodríguez-Hernández, Ana M. Aranda, Miguel A. Truniger, Verónica Nicotiana benthamiana resistance to non-adapted Melon necrotic spot virus results from an incompatible interaction between virus RNA and translation initiation factor 4E |
| description |
10 Pág. |
| author2 |
Ministerio de Ciencia e Innovación (España) |
| author_facet |
Ministerio de Ciencia e Innovación (España) Nieto, Cristina Rodríguez-Moreno, Luis Rodríguez-Hernández, Ana M. Aranda, Miguel A. Truniger, Verónica |
| format |
artículo |
| topic_facet |
cap-independent translational enhancer Melon necrotic spot virus Nicotiana benthamiana Non-host resistance Susceptibility factor Translation initiation factor eIF4E |
| author |
Nieto, Cristina Rodríguez-Moreno, Luis Rodríguez-Hernández, Ana M. Aranda, Miguel A. Truniger, Verónica |
| author_sort |
Nieto, Cristina |
| title |
Nicotiana benthamiana resistance to non-adapted Melon necrotic spot virus results from an incompatible interaction between virus RNA and translation initiation factor 4E |
| title_short |
Nicotiana benthamiana resistance to non-adapted Melon necrotic spot virus results from an incompatible interaction between virus RNA and translation initiation factor 4E |
| title_full |
Nicotiana benthamiana resistance to non-adapted Melon necrotic spot virus results from an incompatible interaction between virus RNA and translation initiation factor 4E |
| title_fullStr |
Nicotiana benthamiana resistance to non-adapted Melon necrotic spot virus results from an incompatible interaction between virus RNA and translation initiation factor 4E |
| title_full_unstemmed |
Nicotiana benthamiana resistance to non-adapted Melon necrotic spot virus results from an incompatible interaction between virus RNA and translation initiation factor 4E |
| title_sort |
nicotiana benthamiana resistance to non-adapted melon necrotic spot virus results from an incompatible interaction between virus rna and translation initiation factor 4e |
| publisher |
Wiley-Blackwell |
| publishDate |
2011-01-22 |
| url |
http://hdl.handle.net/10261/345157 http://dx.doi.org/10.13039/501100004837 http://dx.doi.org/10.13039/100007801 https://api.elsevier.com/content/abstract/scopus_id/79955383643 |
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1802819863556653056 |