Chromatin Dynamics Contribute to the Spatiotemporal Expression Pattern of Virulence Genes in a Fungal Plant Pathogen

Chromatin Dynamics Contribute to the Spatiotemporal Expression Pattern of Virulence Genes in a Fungal Plant Pathogen

18 Pág.

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Main Authors: Meile, Lukas, Peter, Jules, Puccetti, Guido, Alassimone, Julien, McDonald, Bruce A., Sánchez-Vallet, Andrea
Other Authors: Swiss National Science Foundation
Format: artículo biblioteca
Language:English
Published: American Society for Microbiology 2020-10-06
Subjects:Chromatin, Effector gene, Filamentous fungi, Histone methylation, Plant pathogens, Reporter gene, Virulence, Wheat,
Online Access:http://hdl.handle.net/10261/344825
https://api.elsevier.com/content/abstract/scopus_id/85092248025
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spelling dig-inia-es-10261-3448252024-05-17T20:38:48Z Chromatin Dynamics Contribute to the Spatiotemporal Expression Pattern of Virulence Genes in a Fungal Plant Pathogen Meile, Lukas Peter, Jules Puccetti, Guido Alassimone, Julien McDonald, Bruce A. Sánchez-Vallet, Andrea Swiss National Science Foundation Meile, Lukas [0000-0002-2680-3309] Peter, Jules [0000-0002-1966-1197] Puccetti, Guido [0000-0002-7747-4306] Alassimone, Julien [0000-0002-8118-2605] McDonald, Bruce A. [0000-0002-5332-2172] Sánchez-Vallet, Andrea [0000-0002-3668-9503] Chromatin Effector gene Filamentous fungi Histone methylation Plant pathogens Reporter gene Virulence Wheat 18 Pág. Dynamic changes in transcription profiles are key for the success of pathogens in colonizing their hosts. In many pathogens, genes associated with virulence, such as effector genes, are located in regions of the genome that are rich in transposable elements and heterochromatin. The contribution of chromatin modifications to gene expression in pathogens remains largely unknown. Using a combination of a reporter gene-based approach and chromatin immunoprecipitation, we show that the heterochromatic environment of effector genes in the fungal plant pathogen Zymoseptoria tritici is a key regulator of their specific spatiotemporal expression patterns. Enrichment in trimethylated lysine 27 of histone H3 dictates the repression of effector genes in the absence of the host. Chromatin decondensation during host colonization, featuring a reduction in this repressive modification, indicates a major role for epigenetics in effector gene induction. Our results illustrate that chromatin modifications triggered during host colonization determine the specific expression profile of effector genes at the cellular level and, hence, provide new insights into the regulation of virulence in fungal plant pathogens.IMPORTANCE Fungal plant pathogens possess a large repertoire of genes encoding putative effectors, which are crucial for infection. Many of these genes are expressed at low levels in the absence of the host but are strongly induced at specific stages of the infection. The mechanisms underlying this transcriptional reprogramming remain largely unknown. We investigated the role of the genomic environment and associated chromatin modifications of effector genes in controlling their expression pattern in the fungal wheat pathogen Zymoseptoria tritici Depending on their genomic location, effector genes are epigenetically repressed in the absence of the host and during the initial stages of infection. Derepression of effector genes occurs mainly during and after penetration of plant leaves and is associated with changes in histone modifications. Our work demonstrates the role of chromatin in shaping the expression of virulence components and, thereby, the interaction between fungal pathogens and their plant hosts. This research was supported by the Swiss National Science Foundation (grant 31003A_155955 to B.A.M.). The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication. Peer reviewed 2024-02-01T12:17:53Z 2024-02-01T12:17:53Z 2020-10-06 artículo http://purl.org/coar/resource_type/c_6501 mBio 11(5): e02343 (2020) 2161-2129 http://hdl.handle.net/10261/344825 10.1128/mBio.02343-20 2150-7511 33024042 2-s2.0-85092248025 https://api.elsevier.com/content/abstract/scopus_id/85092248025 en mBio Publisher's version https://doi.org/10.1128/mbio.02343-20 Sí open application/pdf American Society for Microbiology
institution INIA ES
collection DSpace
country España
countrycode ES
component Bibliográfico
access En linea
databasecode dig-inia-es
tag biblioteca
region Europa del Sur
libraryname Biblioteca del INIA España
language English
topic Chromatin
Effector gene
Filamentous fungi
Histone methylation
Plant pathogens
Reporter gene
Virulence
Wheat
Chromatin
Effector gene
Filamentous fungi
Histone methylation
Plant pathogens
Reporter gene
Virulence
Wheat
spellingShingle Chromatin
Effector gene
Filamentous fungi
Histone methylation
Plant pathogens
Reporter gene
Virulence
Wheat
Chromatin
Effector gene
Filamentous fungi
Histone methylation
Plant pathogens
Reporter gene
Virulence
Wheat
Meile, Lukas
Peter, Jules
Puccetti, Guido
Alassimone, Julien
McDonald, Bruce A.
Sánchez-Vallet, Andrea
Chromatin Dynamics Contribute to the Spatiotemporal Expression Pattern of Virulence Genes in a Fungal Plant Pathogen
description 18 Pág.
author2 Swiss National Science Foundation
author_facet Swiss National Science Foundation
Meile, Lukas
Peter, Jules
Puccetti, Guido
Alassimone, Julien
McDonald, Bruce A.
Sánchez-Vallet, Andrea
format artículo
topic_facet Chromatin
Effector gene
Filamentous fungi
Histone methylation
Plant pathogens
Reporter gene
Virulence
Wheat
author Meile, Lukas
Peter, Jules
Puccetti, Guido
Alassimone, Julien
McDonald, Bruce A.
Sánchez-Vallet, Andrea
author_sort Meile, Lukas
title Chromatin Dynamics Contribute to the Spatiotemporal Expression Pattern of Virulence Genes in a Fungal Plant Pathogen
title_short Chromatin Dynamics Contribute to the Spatiotemporal Expression Pattern of Virulence Genes in a Fungal Plant Pathogen
title_full Chromatin Dynamics Contribute to the Spatiotemporal Expression Pattern of Virulence Genes in a Fungal Plant Pathogen
title_fullStr Chromatin Dynamics Contribute to the Spatiotemporal Expression Pattern of Virulence Genes in a Fungal Plant Pathogen
title_full_unstemmed Chromatin Dynamics Contribute to the Spatiotemporal Expression Pattern of Virulence Genes in a Fungal Plant Pathogen
title_sort chromatin dynamics contribute to the spatiotemporal expression pattern of virulence genes in a fungal plant pathogen
publisher American Society for Microbiology
publishDate 2020-10-06
url http://hdl.handle.net/10261/344825
https://api.elsevier.com/content/abstract/scopus_id/85092248025
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