Strain and growth conditions may regulate resistance of Listeria monocytogenes biofilms to benzalkonium chloride

Strain and growth conditions may regulate resistance of Listeria monocytogenes biofilms to benzalkonium chloride

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Main Authors: Bonneville, Lourenço, Ortiz, Sagrario, Maia, Vera, Brito, Luisa, Martínez Suarez, Joaquín V.
Other Authors: Agencia Estatal de Investigación (España)
Format: artículo biblioteca
Language:English
Published: Multidisciplinary Digital Publishing Institute 2020-02-03
Subjects:Benzalkonium chloride, Biofilm, Disinfectant, Food industry, Listeria monocytogenes, Persistence, Polystyrene, Resistance, Stainless steel,
Online Access:http://hdl.handle.net/10261/343673
http://dx.doi.org/10.13039/501100000780
http://dx.doi.org/10.13039/501100011033
https://api.elsevier.com/content/abstract/scopus_id/85081604577
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spelling dig-inia-es-10261-3436732024-05-16T20:51:17Z Strain and growth conditions may regulate resistance of Listeria monocytogenes biofilms to benzalkonium chloride Bonneville, Lourenço Ortiz, Sagrario Maia, Vera Brito, Luisa Martínez Suarez, Joaquín V. Agencia Estatal de Investigación (España) Ministerio de Ciencia, Innovación y Universidades (España) European Commission Bonneville, Lourenço [0000-0002-7092-9021] Ortiz, Sagrario [0000-0002-7051-5128] Maia, Vera [0000-0001-9101-8801] Brito, Luisa [0000-0002-8614-5184] Martínez Suarez, Joaquín V. [0000-0003-4306-3223] Benzalkonium chloride Biofilm Disinfectant Food industry Listeria monocytogenes Persistence Polystyrene Resistance Stainless steel 10 Pág. Listeria monocytogenes is one of the main foodborne pathogens. The formation of biofilms by L. monocytogenes contributes to its resistance to disinfectants, which represents a serious risk for food production plants. The aim of this study was to compare the effect of sub-inhibitory concentrations of benzalkonium chloride (BAC) (1.25 or 2.5 mg/L) on biofilm production and on biofilm reduction after exposure to an inhibitory concentration of BAC (1280 mg/L) in two isogenic L. monocytogenes strains: the BAC-sensitive wild-type strain S2-1 and its BAC-resistant mutant derivative S2BAC, which presented a multidrug resistance phenotype. The biofilm-forming ability of the strains under different BAC concentrations was evaluated by the resazurin method using polystyrene microplates. The biofilm reduction after BAC exposure was evaluated by using stainless steel coupons (SSCs). When the resazurin method was used, S2BAC produced significantly more biofilm in the presence of a sub-inhibitory concentration of BAC compared to that in the culture medium without BAC (p < 0.05). When the SSC method was used, the presence of sub-inhibitory concentrations of BAC resulted in a higher resistance of the biofilm for S2BAC compared to that in the culture medium without BAC (p < 0.05). This was not observed with the sensitive S2-1 strain. These results suggest that biofilm behavior depends on the strain and sub-inhibitory concentrations of disinfectants and may explain the ability of certain isolates to persist in niches of food processing plants. This research was funded by Research Project grant RTI2018-098267-R-C31 from the Spanish Ministry of Science, Innovation and Universities and by project PORBIOTA-Portuguese E-Infrastructure for Information and Research on Biodiversity (POCI-01-0145-FEDER-022127), funded by Operational Thematic Program for Competitiveness and Internationalization (POCI), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (FEDER). Peer reviewed 2024-01-25T09:20:45Z 2024-01-25T09:20:45Z 2020-02-03 artículo http://purl.org/coar/resource_type/c_6501 Applied Sciences 10(3): e988 (2020) http://hdl.handle.net/10261/343673 10.3390/app10030988 2076-3417 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100011033 2-s2.0-85081604577 https://api.elsevier.com/content/abstract/scopus_id/85081604577 en #PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-098267-R-C31/ES/ALTERNATIVAS AL USO DE DESINFECTANTES EN LA INDUSTRIA ALIMENTARIA DIRIGIDAS A REDUCIR LA SUPERVIVENCIA DE LISTERIA MONOCYTOGENES Y SALMONELLA ENTERICA SOBRE LAS SUPERFICIES/ info:eu-repo/grantAgreement/EC/022127 Departamento de Tecnología de los alimentos Publisher's version https://doi.org/10.3390/app10030988 Sí open application/pdf Multidisciplinary Digital Publishing Institute
institution INIA ES
collection DSpace
country España
countrycode ES
component Bibliográfico
access En linea
databasecode dig-inia-es
tag biblioteca
region Europa del Sur
libraryname Biblioteca del INIA España
language English
topic Benzalkonium chloride
Biofilm
Disinfectant
Food industry
Listeria monocytogenes
Persistence
Polystyrene
Resistance
Stainless steel
Benzalkonium chloride
Biofilm
Disinfectant
Food industry
Listeria monocytogenes
Persistence
Polystyrene
Resistance
Stainless steel
spellingShingle Benzalkonium chloride
Biofilm
Disinfectant
Food industry
Listeria monocytogenes
Persistence
Polystyrene
Resistance
Stainless steel
Benzalkonium chloride
Biofilm
Disinfectant
Food industry
Listeria monocytogenes
Persistence
Polystyrene
Resistance
Stainless steel
Bonneville, Lourenço
Ortiz, Sagrario
Maia, Vera
Brito, Luisa
Martínez Suarez, Joaquín V.
Strain and growth conditions may regulate resistance of Listeria monocytogenes biofilms to benzalkonium chloride
description 10 Pág.
author2 Agencia Estatal de Investigación (España)
author_facet Agencia Estatal de Investigación (España)
Bonneville, Lourenço
Ortiz, Sagrario
Maia, Vera
Brito, Luisa
Martínez Suarez, Joaquín V.
format artículo
topic_facet Benzalkonium chloride
Biofilm
Disinfectant
Food industry
Listeria monocytogenes
Persistence
Polystyrene
Resistance
Stainless steel
author Bonneville, Lourenço
Ortiz, Sagrario
Maia, Vera
Brito, Luisa
Martínez Suarez, Joaquín V.
author_sort Bonneville, Lourenço
title Strain and growth conditions may regulate resistance of Listeria monocytogenes biofilms to benzalkonium chloride
title_short Strain and growth conditions may regulate resistance of Listeria monocytogenes biofilms to benzalkonium chloride
title_full Strain and growth conditions may regulate resistance of Listeria monocytogenes biofilms to benzalkonium chloride
title_fullStr Strain and growth conditions may regulate resistance of Listeria monocytogenes biofilms to benzalkonium chloride
title_full_unstemmed Strain and growth conditions may regulate resistance of Listeria monocytogenes biofilms to benzalkonium chloride
title_sort strain and growth conditions may regulate resistance of listeria monocytogenes biofilms to benzalkonium chloride
publisher Multidisciplinary Digital Publishing Institute
publishDate 2020-02-03
url http://hdl.handle.net/10261/343673
http://dx.doi.org/10.13039/501100000780
http://dx.doi.org/10.13039/501100011033
https://api.elsevier.com/content/abstract/scopus_id/85081604577
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AT maiavera strainandgrowthconditionsmayregulateresistanceoflisteriamonocytogenesbiofilmstobenzalkoniumchloride
AT britoluisa strainandgrowthconditionsmayregulateresistanceoflisteriamonocytogenesbiofilmstobenzalkoniumchloride
AT martinezsuarezjoaquinv strainandgrowthconditionsmayregulateresistanceoflisteriamonocytogenesbiofilmstobenzalkoniumchloride
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