Arabinoxylan-Oligosaccharides Act as Damage Associated Molecular Patterns in Plants Regulating Disease Resistance
Immune responses in plants can be triggered by damage/microbe-associated molecular patterns (DAMPs/MAMPs) upon recognition by plant pattern recognition receptors (PRRs). DAMPs are signaling molecules synthesized by plants or released from host cellular structures (e.g., plant cell walls) upon pathogen infection or wounding. Despite the hypothesized important role of plant cell wall-derived DAMPs in plant-pathogen interactions, a very limited number of these DAMPs are well characterized. Recent work demonstrated that pectin-enriched cell wall fractions extracted from the cell wall mutant impaired in Arabidopsis Response Regulator 6 (arr6), that showed altered disease resistance to several pathogens, triggered more intense immune responses than those activated by similar cell wall fractions from wild-type plants. It was hypothesized that arr6 cell wall fractions could be differentially enriched in DAMPs. In this work, we describe the characterization of the previous immune-active fractions of arr6 showing the highest triggering capacities upon further fractionation by chromatographic means. These analyses pointed to a role of pentose-based oligosaccharides triggering plant immune responses. The characterization of several pentose-based oligosaccharide structures revealed that β-1,4-xylooligosaccharides of specific degrees of polymerization and carrying arabinose decorations are sensed as DAMPs by plants. Moreover, the pentasaccharide 33-α-L-arabinofuranosyl-xylotetraose (XA3XX) was found as a highly active DAMP structure triggering strong immune responses in Arabidopsis thaliana and enhancing crop disease resistance.
Main Authors: | , , , , , , , , |
---|---|
Other Authors: | |
Format: | artículo biblioteca |
Language: | English |
Published: |
Frontiers Media
2020-08-07
|
Subjects: | Arabinoxylan, Cell wall, Damage-associated molecular pattern (DAMP), Pattern triggered immunity, Plant immunity, |
Online Access: | http://hdl.handle.net/10261/339134 http://dx.doi.org/10.13039/501100003329 http://dx.doi.org/10.13039/501100001659 http://dx.doi.org/10.13039/100012818 http://dx.doi.org/10.13039/501100011033 http://dx.doi.org/10.13039/501100004189 https://api.elsevier.com/content/abstract/scopus_id/85089896778 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
id |
dig-inia-es-10261-339134 |
---|---|
record_format |
koha |
institution |
INIA ES |
collection |
DSpace |
country |
España |
countrycode |
ES |
component |
Bibliográfico |
access |
En linea |
databasecode |
dig-inia-es |
tag |
biblioteca |
region |
Europa del Sur |
libraryname |
Biblioteca del INIA España |
language |
English |
topic |
Arabinoxylan Cell wall Damage-associated molecular pattern (DAMP) Pattern triggered immunity Plant immunity Arabinoxylan Cell wall Damage-associated molecular pattern (DAMP) Pattern triggered immunity Plant immunity |
spellingShingle |
Arabinoxylan Cell wall Damage-associated molecular pattern (DAMP) Pattern triggered immunity Plant immunity Arabinoxylan Cell wall Damage-associated molecular pattern (DAMP) Pattern triggered immunity Plant immunity Mélida, Hugo Bacete, Laura Ruprecht, Colin Rebaque, Diego Del Hierro, Irene López, Gema Brunner, Frédéric Pfrengle, Fabian Molina, Antonio Arabinoxylan-Oligosaccharides Act as Damage Associated Molecular Patterns in Plants Regulating Disease Resistance |
description |
Immune responses in plants can be triggered by damage/microbe-associated molecular patterns (DAMPs/MAMPs) upon recognition by plant pattern recognition receptors (PRRs). DAMPs are signaling molecules synthesized by plants or released from host cellular structures (e.g., plant cell walls) upon pathogen infection or wounding. Despite the hypothesized important role of plant cell wall-derived DAMPs in plant-pathogen interactions, a very limited number of these DAMPs are well characterized. Recent work demonstrated that pectin-enriched cell wall fractions extracted from the cell wall mutant impaired in Arabidopsis Response Regulator 6 (arr6), that showed altered disease resistance to several pathogens, triggered more intense immune responses than those activated by similar cell wall fractions from wild-type plants. It was hypothesized that arr6 cell wall fractions could be differentially enriched in DAMPs. In this work, we describe the characterization of the previous immune-active fractions of arr6 showing the highest triggering capacities upon further fractionation by chromatographic means. These analyses pointed to a role of pentose-based oligosaccharides triggering plant immune responses. The characterization of several pentose-based oligosaccharide structures revealed that β-1,4-xylooligosaccharides of specific degrees of polymerization and carrying arabinose decorations are sensed as DAMPs by plants. Moreover, the pentasaccharide 33-α-L-arabinofuranosyl-xylotetraose (XA3XX) was found as a highly active DAMP structure triggering strong immune responses in Arabidopsis thaliana and enhancing crop disease resistance. |
author2 |
Comunidad de Madrid |
author_facet |
Comunidad de Madrid Mélida, Hugo Bacete, Laura Ruprecht, Colin Rebaque, Diego Del Hierro, Irene López, Gema Brunner, Frédéric Pfrengle, Fabian Molina, Antonio |
format |
artículo |
topic_facet |
Arabinoxylan Cell wall Damage-associated molecular pattern (DAMP) Pattern triggered immunity Plant immunity |
author |
Mélida, Hugo Bacete, Laura Ruprecht, Colin Rebaque, Diego Del Hierro, Irene López, Gema Brunner, Frédéric Pfrengle, Fabian Molina, Antonio |
author_sort |
Mélida, Hugo |
title |
Arabinoxylan-Oligosaccharides Act as Damage Associated Molecular Patterns in Plants Regulating Disease Resistance |
title_short |
Arabinoxylan-Oligosaccharides Act as Damage Associated Molecular Patterns in Plants Regulating Disease Resistance |
title_full |
Arabinoxylan-Oligosaccharides Act as Damage Associated Molecular Patterns in Plants Regulating Disease Resistance |
title_fullStr |
Arabinoxylan-Oligosaccharides Act as Damage Associated Molecular Patterns in Plants Regulating Disease Resistance |
title_full_unstemmed |
Arabinoxylan-Oligosaccharides Act as Damage Associated Molecular Patterns in Plants Regulating Disease Resistance |
title_sort |
arabinoxylan-oligosaccharides act as damage associated molecular patterns in plants regulating disease resistance |
publisher |
Frontiers Media |
publishDate |
2020-08-07 |
url |
http://hdl.handle.net/10261/339134 http://dx.doi.org/10.13039/501100003329 http://dx.doi.org/10.13039/501100001659 http://dx.doi.org/10.13039/100012818 http://dx.doi.org/10.13039/501100011033 http://dx.doi.org/10.13039/501100004189 https://api.elsevier.com/content/abstract/scopus_id/85089896778 |
work_keys_str_mv |
AT melidahugo arabinoxylanoligosaccharidesactasdamageassociatedmolecularpatternsinplantsregulatingdiseaseresistance AT bacetelaura arabinoxylanoligosaccharidesactasdamageassociatedmolecularpatternsinplantsregulatingdiseaseresistance AT ruprechtcolin arabinoxylanoligosaccharidesactasdamageassociatedmolecularpatternsinplantsregulatingdiseaseresistance AT rebaquediego arabinoxylanoligosaccharidesactasdamageassociatedmolecularpatternsinplantsregulatingdiseaseresistance AT delhierroirene arabinoxylanoligosaccharidesactasdamageassociatedmolecularpatternsinplantsregulatingdiseaseresistance AT lopezgema arabinoxylanoligosaccharidesactasdamageassociatedmolecularpatternsinplantsregulatingdiseaseresistance AT brunnerfrederic arabinoxylanoligosaccharidesactasdamageassociatedmolecularpatternsinplantsregulatingdiseaseresistance AT pfrenglefabian arabinoxylanoligosaccharidesactasdamageassociatedmolecularpatternsinplantsregulatingdiseaseresistance AT molinaantonio arabinoxylanoligosaccharidesactasdamageassociatedmolecularpatternsinplantsregulatingdiseaseresistance |
_version_ |
1802819814075400192 |
spelling |
dig-inia-es-10261-3391342024-05-17T20:38:19Z Arabinoxylan-Oligosaccharides Act as Damage Associated Molecular Patterns in Plants Regulating Disease Resistance Mélida, Hugo Bacete, Laura Ruprecht, Colin Rebaque, Diego Del Hierro, Irene López, Gema Brunner, Frédéric Pfrengle, Fabian Molina, Antonio Comunidad de Madrid Ministerio de Economía y Competitividad (España) Ministerio de Ciencia, Innovación y Universidades (España) Agencia Estatal de Investigación (España) Ministerio de Educación (España) Max Planck Society German Research Foundation Mélida, Hugo [0000-0003-1792-0113] Bacete, Laura [0000-0003-3171-8181] Ruprecht, Colin [0000-0003-4362-2208] Rebaque, Diego [0000-0001-5343-7220] Del Hierro, Irene [0000-0001-7777-0802] Pfrengle, Fabian [0000-0003-2206-6636] López, Gema [0000-0003-2588-4378] Arabinoxylan Cell wall Damage-associated molecular pattern (DAMP) Pattern triggered immunity Plant immunity Immune responses in plants can be triggered by damage/microbe-associated molecular patterns (DAMPs/MAMPs) upon recognition by plant pattern recognition receptors (PRRs). DAMPs are signaling molecules synthesized by plants or released from host cellular structures (e.g., plant cell walls) upon pathogen infection or wounding. Despite the hypothesized important role of plant cell wall-derived DAMPs in plant-pathogen interactions, a very limited number of these DAMPs are well characterized. Recent work demonstrated that pectin-enriched cell wall fractions extracted from the cell wall mutant impaired in Arabidopsis Response Regulator 6 (arr6), that showed altered disease resistance to several pathogens, triggered more intense immune responses than those activated by similar cell wall fractions from wild-type plants. It was hypothesized that arr6 cell wall fractions could be differentially enriched in DAMPs. In this work, we describe the characterization of the previous immune-active fractions of arr6 showing the highest triggering capacities upon further fractionation by chromatographic means. These analyses pointed to a role of pentose-based oligosaccharides triggering plant immune responses. The characterization of several pentose-based oligosaccharide structures revealed that β-1,4-xylooligosaccharides of specific degrees of polymerization and carrying arabinose decorations are sensed as DAMPs by plants. Moreover, the pentasaccharide 33-α-L-arabinofuranosyl-xylotetraose (XA3XX) was found as a highly active DAMP structure triggering strong immune responses in Arabidopsis thaliana and enhancing crop disease resistance. This work was supported by grants IND2017/BIO-7800 of the Comunidad de Madrid Regional Government, BIO2015-64077-R of the Spanish Ministry of Economy and Competitiveness (MINECO), RTI2018-096975-B-I00 of Spanish Ministry of Science, Innovation and Universities, to AM. This work has been also financially supported by the “Severo Ochoa Programme for Centres of Excellence in R&D” from the Agencia Estatal de Investigación of Spain (grant SEV-2016-0672 (2017-2021) to the CBGP). In the frame of this program HM was supported with a postdoctoral fellow. DR was the recipient of an Industrial PhD Fellow (IND2017/BIO-7800) and IH was the recipient of an PhD FPU fellow from the Spanish Ministry of Education (FPU16/07118). FP thanks the Max Planck Society and the German Research Foundation (DFG, Emmy Noether program PF850/1-1 to FP) for financial support. Peer reviewed 2023-11-17T10:11:06Z 2023-11-17T10:11:06Z 2020-08-07 artículo http://purl.org/coar/resource_type/c_6501 Frontiers in Plant Science 11: e1210 (2020) http://hdl.handle.net/10261/339134 10.3389/fpls.2020.01210 1664-462X http://dx.doi.org/10.13039/501100003329 http://dx.doi.org/10.13039/501100001659 http://dx.doi.org/10.13039/100012818 http://dx.doi.org/10.13039/501100011033 http://dx.doi.org/10.13039/501100004189 32849751 2-s2.0-85089896778 https://api.elsevier.com/content/abstract/scopus_id/85089896778 en #PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# S2017/BIO-7800 info:eu-repo/grantAgreement/MINECO//BIO2015-64077-R/ES/RESPUESTAS DE INMUNIDAD REGULADAS POR EL SISTEMA DE PERCEPCION DE INTEGRIDAD DE LA PARED CELULAR VEGETAL/ info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-096975-B-I00/ES/RESPUESTAS DE INMUNIDAD VEGETAL REGULADAS POR PATRONES MOLECULARES ASOCIADOS A DAÑO: DEL RECONOCIMIENTO DE DAMPS (DAMAGE-ASSOCIATED MOLECULAR PATTERNS) A LA RESISTENCIA/ info:eu-repo/grantAgreement/MEC//FPU16/07118 Centro de Biotecnología y Genómica de Plantas (CBGP) Publisher's version Sí open application/pdf Frontiers Media |