D-Chiro-Inositol Treatment Affects Oocyte and Embryo Quality and Improves Glucose Intolerance in Both Aged Mice and Mouse Models of Polycystic Ovarian Syndrome
19 Pág.
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| Format: | artículo biblioteca |
| Language: | English |
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Multidisciplinary Digital Publishing Institute
2020-08-22
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| Subjects: | D-chiro-inositol, Embryo, Fertility, Glucose intolerance, Oocyte, Oxidative stress, Polycystic ovary syndrome, |
| Online Access: | http://hdl.handle.net/10261/338891 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100004837 http://dx.doi.org/10.13039/501100011011 http://dx.doi.org/10.13039/501100004587 https://api.elsevier.com/content/abstract/scopus_id/85089925262 |
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D-chiro-inositol Embryo Fertility Glucose intolerance Oocyte Oxidative stress Polycystic ovary syndrome D-chiro-inositol Embryo Fertility Glucose intolerance Oocyte Oxidative stress Polycystic ovary syndrome |
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D-chiro-inositol Embryo Fertility Glucose intolerance Oocyte Oxidative stress Polycystic ovary syndrome D-chiro-inositol Embryo Fertility Glucose intolerance Oocyte Oxidative stress Polycystic ovary syndrome Pericuesta Camacho, Eva Laguna-Barraza, Ricardo Ramos Ibeas, Priscila Gutierrez-Arroyo, Julia Navarro, Juán Antonio Vera, Katia Sanjuan, Carlos Baixeras, Elena Rodriguez de Fonseca, F. Gutiérrez-Adán, Alfonso D-Chiro-Inositol Treatment Affects Oocyte and Embryo Quality and Improves Glucose Intolerance in Both Aged Mice and Mouse Models of Polycystic Ovarian Syndrome |
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19 Pág. |
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Ministerio de Ciencia e Innovación (España) |
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Ministerio de Ciencia e Innovación (España) Pericuesta Camacho, Eva Laguna-Barraza, Ricardo Ramos Ibeas, Priscila Gutierrez-Arroyo, Julia Navarro, Juán Antonio Vera, Katia Sanjuan, Carlos Baixeras, Elena Rodriguez de Fonseca, F. Gutiérrez-Adán, Alfonso |
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artículo |
| topic_facet |
D-chiro-inositol Embryo Fertility Glucose intolerance Oocyte Oxidative stress Polycystic ovary syndrome |
| author |
Pericuesta Camacho, Eva Laguna-Barraza, Ricardo Ramos Ibeas, Priscila Gutierrez-Arroyo, Julia Navarro, Juán Antonio Vera, Katia Sanjuan, Carlos Baixeras, Elena Rodriguez de Fonseca, F. Gutiérrez-Adán, Alfonso |
| author_sort |
Pericuesta Camacho, Eva |
| title |
D-Chiro-Inositol Treatment Affects Oocyte and Embryo Quality and Improves Glucose Intolerance in Both Aged Mice and Mouse Models of Polycystic Ovarian Syndrome |
| title_short |
D-Chiro-Inositol Treatment Affects Oocyte and Embryo Quality and Improves Glucose Intolerance in Both Aged Mice and Mouse Models of Polycystic Ovarian Syndrome |
| title_full |
D-Chiro-Inositol Treatment Affects Oocyte and Embryo Quality and Improves Glucose Intolerance in Both Aged Mice and Mouse Models of Polycystic Ovarian Syndrome |
| title_fullStr |
D-Chiro-Inositol Treatment Affects Oocyte and Embryo Quality and Improves Glucose Intolerance in Both Aged Mice and Mouse Models of Polycystic Ovarian Syndrome |
| title_full_unstemmed |
D-Chiro-Inositol Treatment Affects Oocyte and Embryo Quality and Improves Glucose Intolerance in Both Aged Mice and Mouse Models of Polycystic Ovarian Syndrome |
| title_sort |
d-chiro-inositol treatment affects oocyte and embryo quality and improves glucose intolerance in both aged mice and mouse models of polycystic ovarian syndrome |
| publisher |
Multidisciplinary Digital Publishing Institute |
| publishDate |
2020-08-22 |
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http://hdl.handle.net/10261/338891 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100004837 http://dx.doi.org/10.13039/501100011011 http://dx.doi.org/10.13039/501100004587 https://api.elsevier.com/content/abstract/scopus_id/85089925262 |
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dig-inia-es-10261-3388912024-10-27T21:40:12Z D-Chiro-Inositol Treatment Affects Oocyte and Embryo Quality and Improves Glucose Intolerance in Both Aged Mice and Mouse Models of Polycystic Ovarian Syndrome Pericuesta Camacho, Eva Laguna-Barraza, Ricardo Ramos Ibeas, Priscila Gutierrez-Arroyo, Julia Navarro, Juán Antonio Vera, Katia Sanjuan, Carlos Baixeras, Elena Rodriguez de Fonseca, F. Gutiérrez-Adán, Alfonso Ministerio de Ciencia e Innovación (España) Instituto de Salud Carlos III European Commission Junta de Andalucía Pericuesta Camacho, Eva [0000-0001-9387-3270] Ramos Ibeas, Priscila [0000-0003-4578-0189] Navarro, Juán Antonio [0000-0003-3089-0983] Baixeras, Elena [0000-0001-6955-2434] Rodriguez de Fonseca, F. [0000-0002-4516-5795] Gutiérrez-Adán, Alfonso [0000-0001-9893-9179] Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72] D-chiro-inositol Embryo Fertility Glucose intolerance Oocyte Oxidative stress Polycystic ovary syndrome 19 Pág. Polycystic ovarian syndrome (PCOS) is the main cause of female infertility. It is a multifactorial disorder with varying clinical manifestations including metabolic/endocrine abnormalities, hyperandrogenism, and ovarian cysts, among other conditions. D-Chiro-inositol (DCI) is the main treatment available for PCOS in humans. To address some of the mechanisms of this complex disorder and its treatment, this study examines the effect of DCI on reproduction during the development of different PCOS-associated phenotypes in aged females and two mouse models of PCOS. Aged females (8 months old) were treated or not (control) with DCI for 2 months. PCOS models were generated by treatment with dihydrotestosterone (DHT) on Days 16, 17, and 18 of gestation, or by testosterone propionate (TP) treatment on the first day of life. At two months of age, PCOS mice were treated with DCI for 2 months and their reproductive parameters analyzed. No effects of DCI treatment were produced on body weight or ovary/body weight ratio. However, treatment reduced the number of follicles with an atretic cyst-like appearance and improved embryo development in the PCOS models, and also increased implantation rates in both aged and PCOS mice. DCI modified the expression of genes related to oocyte quality, oxidative stress, and luteal sufficiency in cumulus-oocyte complexes (COCs) obtained from the aged and PCOS models. Further, the phosphorylation of AKT, a main metabolic sensor activated by insulin in the liver, was enhanced only in the DHT group, which was the only PCOS model showing glucose intolerance and AKT dephosphorylation. The effect of DCI in the TP model seemed mediated by its influence on oxidative stress and follicle insufficiency. Our results indicate that DCI works in preclinical models of PCOS and offer insight into its mechanism of action when used to treat this infertility-associated syndrome. This work was supported by grants RTC2016-4983-1 and RTI2018-093548-B-I00 from the Spanish Ministry of Science and Innovation, and from the EU-ERDF-Instituto de Salud Carlos III (Grant PI19/01577) and Consejería de Economía, Conocimiento y Universidad, Junta de Andalucía (Grant P18-TP-5194). P Ramos-Ibeas was supported by a Ramón y Cajal Contract from MINECO (RYC2018-025666-I). Peer reviewed 2023-11-14T09:03:20Z 2023-11-14T09:03:20Z 2020-08-22 artículo http://purl.org/coar/resource_type/c_6501 International Journal of Molecular Sciences 21(17): e6049 (2020) 1661-6596 http://hdl.handle.net/10261/338891 10.3390/ijms21176049 1422-0067 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100004837 http://dx.doi.org/10.13039/501100011011 http://dx.doi.org/10.13039/501100004587 32842637 2-s2.0-85089925262 https://api.elsevier.com/content/abstract/scopus_id/85089925262 en #PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/AEI//RTC2016-4983-1 info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-093548-B-I00/ES/ANALISIS FUNCIONAL DE ISOFORMAS DE ARNM RELACIONADAS CON DESARROLLO EMBRIONARIO Y FERTILIDAD/ info:eu-repo/grantAgreement/MINECO//RYC2018-025666-I Departamento de Reproducción Animal Publisher's version https://doi.org/10.3390/ijms21176049 Sí open application/pdf Multidisciplinary Digital Publishing Institute |