Two serological approaches for detection of antibodies to SARS-CoV-2 in different scenarios: a screening tool and a point-of-care test
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| Format: | artículo biblioteca |
| Language: | English |
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Elsevier
2020-12
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| Subjects: | COVID-19, Diagnosis, ELISA, LFA, SARS-CoV-2, Serology, |
| Online Access: | http://hdl.handle.net/10261/337345 http://dx.doi.org/10.13039/501100000780 https://api.elsevier.com/content/abstract/scopus_id/85090043845 |
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dig-inia-es-10261-3373452024-10-26T20:43:08Z Two serological approaches for detection of antibodies to SARS-CoV-2 in different scenarios: a screening tool and a point-of-care test Hoste, Alexis C.R. Venteo, Ángel Fresco-Taboada, Alba Tapia, Istar Monedero, Alejandro López, Lissette Jebbink, Maarten F. Pérez-Ramírez, E. Jiménez-Clavero, Miguel Ángel Almonacid, Mercedes Muñóz, Patricia E. Guinea, Jesus Vela, Carmen van der Hoek, Lia Rueda, Paloma Sastre, Patricia European Commission Hoste, Alexis C.R. [0000-0001-6724-6062] Venteo, Ángel [0000-0002-3680-5906] Fresco-Taboada, Alba [0000-0001-7103-8522] Jebbink, Maarten F. [0000-0001-5937-7548] Pérez-Ramírez, E. [0000-0001-7701-9816] Jiménez-Clavero, Miguel Ángel [0000-0003-2125-9743] Almonacid, Mercedes [0000-0002-8191-647X] Guinea, Jesus [0000-0002-7901-8355] Sastre, Patricia [0000-0003-3732-5047] Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72] COVID-19 Diagnosis ELISA LFA SARS-CoV-2 Serology 5 Pág. Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has infected more than 8 million people worldwide, becoming a pandemic. Detecting antibodies against SARS-CoV-2 is of utmost importance and a good indicator of exposure and circulation of the virus within the general population. Two serological tools based on a double recognition assay [enzyme-linked immunosorbent assay (DR-ELISA) and lateral flow assay (DR-LFA)] to detect total antibodies to SARS-CoV-2 have been developed based on the recombinant nucleocapsid protein. A total of 1065 serum samples, including positive for COVID-19 and negative samples from healthy donors or infected with other respiratory pathogens, were analyzed. The results showed values of sensitivity between 91.2% and 100%, and specificity of 100% and 98.2% for DR-LFA and DR-ELISA, respectively. No cross-reactivity against seasonal coronavirus (HCoV-NL63, HCoV-229E, HCoV-HKU1, HCoV-OC43) was found. These results demonstrate the importance of serology as a complementary tool to polymerase chain reaction for follow-up of recovered patients and identification of asymptomatic individuals. This work was supported by the EU Marie Skłodowska-Curie Actions Innovative Training Network HONOURs under grant no. 721367 (to Alexis C.R. Hoste). Peer reviewed 2023-10-18T10:40:36Z 2023-10-18T10:40:36Z 2020-12 artículo http://purl.org/coar/resource_type/c_6501 Diagnostic Microbiology and Infectious Disease 98(4): e115167 (2020) 0732-8893 http://hdl.handle.net/10261/337345 10.1016/j.diagmicrobio.2020.115167 http://dx.doi.org/10.13039/501100000780 32890908 2-s2.0-85090043845 https://api.elsevier.com/content/abstract/scopus_id/85090043845 en #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/EC/721367 Centro de Investigación en Sanidad Animal (CISA) Publisher's version https://doi.org/10.1016/j.diagmicrobio.2020.115167 Sí open application/pdf Elsevier |
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COVID-19 Diagnosis ELISA LFA SARS-CoV-2 Serology COVID-19 Diagnosis ELISA LFA SARS-CoV-2 Serology |
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COVID-19 Diagnosis ELISA LFA SARS-CoV-2 Serology COVID-19 Diagnosis ELISA LFA SARS-CoV-2 Serology Hoste, Alexis C.R. Venteo, Ángel Fresco-Taboada, Alba Tapia, Istar Monedero, Alejandro López, Lissette Jebbink, Maarten F. Pérez-Ramírez, E. Jiménez-Clavero, Miguel Ángel Almonacid, Mercedes Muñóz, Patricia E. Guinea, Jesus Vela, Carmen van der Hoek, Lia Rueda, Paloma Sastre, Patricia Two serological approaches for detection of antibodies to SARS-CoV-2 in different scenarios: a screening tool and a point-of-care test |
| description |
5 Pág. |
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European Commission |
| author_facet |
European Commission Hoste, Alexis C.R. Venteo, Ángel Fresco-Taboada, Alba Tapia, Istar Monedero, Alejandro López, Lissette Jebbink, Maarten F. Pérez-Ramírez, E. Jiménez-Clavero, Miguel Ángel Almonacid, Mercedes Muñóz, Patricia E. Guinea, Jesus Vela, Carmen van der Hoek, Lia Rueda, Paloma Sastre, Patricia |
| format |
artículo |
| topic_facet |
COVID-19 Diagnosis ELISA LFA SARS-CoV-2 Serology |
| author |
Hoste, Alexis C.R. Venteo, Ángel Fresco-Taboada, Alba Tapia, Istar Monedero, Alejandro López, Lissette Jebbink, Maarten F. Pérez-Ramírez, E. Jiménez-Clavero, Miguel Ángel Almonacid, Mercedes Muñóz, Patricia E. Guinea, Jesus Vela, Carmen van der Hoek, Lia Rueda, Paloma Sastre, Patricia |
| author_sort |
Hoste, Alexis C.R. |
| title |
Two serological approaches for detection of antibodies to SARS-CoV-2 in different scenarios: a screening tool and a point-of-care test |
| title_short |
Two serological approaches for detection of antibodies to SARS-CoV-2 in different scenarios: a screening tool and a point-of-care test |
| title_full |
Two serological approaches for detection of antibodies to SARS-CoV-2 in different scenarios: a screening tool and a point-of-care test |
| title_fullStr |
Two serological approaches for detection of antibodies to SARS-CoV-2 in different scenarios: a screening tool and a point-of-care test |
| title_full_unstemmed |
Two serological approaches for detection of antibodies to SARS-CoV-2 in different scenarios: a screening tool and a point-of-care test |
| title_sort |
two serological approaches for detection of antibodies to sars-cov-2 in different scenarios: a screening tool and a point-of-care test |
| publisher |
Elsevier |
| publishDate |
2020-12 |
| url |
http://hdl.handle.net/10261/337345 http://dx.doi.org/10.13039/501100000780 https://api.elsevier.com/content/abstract/scopus_id/85090043845 |
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