Interannual dynamics of Tuber melanosporum and fungal communities in productive black truffle orchards amended with truffle nests
Truffle growers devote great efforts to improve black truffle productivity, developing agronomic practices such as 'truffle nests' (peat amendments that are supplemented with truffle spore inoculum). It has been hypothesized that improved fruiting associated with nests is linked to stimulation of truffle mycelia previously established in soil or to changes generated in soil fungal community. To assess this, we used real-time PCR to quantify black truffle extraradical mycelium during 2 years after nests installation. We also characterized the fungal community via high-throughput amplicon sequencing of the ITS region of rRNA genes. We found that neither the abundance of truffle mycelium in nests nor in the soil-nest interphase was higher than in the bulk soil, which indicates that nests do not improve mycelial growth. The fungal community in nests showed lower richness and Shannon index and was compositionally different from that of soil, which suggests that nests may act as an open niche for fungal colonization that facilitates truffle fruiting. The ectomycorrhizal fungal community showed lower richness in nests. However, no negative relationships between amount of truffle mycelium and reads of other ectomycorrhizal fungi were found, thus countering the hypothesis that ectomycorrhizal competition plays a role in the nest effect.
Main Authors: | , , , , , , , |
---|---|
Other Authors: | |
Format: | artículo biblioteca |
Language: | English |
Published: |
Oxford University Press
2023-08-01
|
Subjects: | Tuber melanosporum, ITS amplicon sequencing, Ectomycorrhizal fungi, Extraradical mycelium, Fungal community, Truffle nests, Strengthen the means of implementation and revitalize the Global Partnership for Sustainable Development, |
Online Access: | http://hdl.handle.net/10261/334909 http://dx.doi.org/10.13039/100000001 https://api.elsevier.com/content/abstract/scopus_id/85166376613 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
id |
dig-inia-es-10261-334909 |
---|---|
record_format |
koha |
spelling |
dig-inia-es-10261-3349092024-05-25T20:41:43Z Interannual dynamics of Tuber melanosporum and fungal communities in productive black truffle orchards amended with truffle nests Garcia-Barreda, Sergi Marco, Pedro Bonito, Gregory Parladé, Javier Sánchez, Sergio González, Vicente Larena, Inmaculada Benucci, Gian Maria Niccolò CSIC - Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA) Ministerio de Ciencia, Innovación y Universidades (España) National Science Foundation (US) Garcia-Barreda, Sergi [0000-0002-7248-234X] Marco, Pedro [0000-0003-3384-7534] Bonito, Gregory [0000-0002-7262-8978] Parladé, Javier [0000-0002-0867-3280] Sánchez, Sergio [0000-0003-4331-9794] González, Vicente [0000-0002-2535-6914] Larena, Inmaculada [0000-0001-8424-8916] Benucci, Gian Maria Niccolò [0000-0003-1589-947X] Tuber melanosporum ITS amplicon sequencing Ectomycorrhizal fungi Extraradical mycelium Fungal community Truffle nests Strengthen the means of implementation and revitalize the Global Partnership for Sustainable Development Truffle growers devote great efforts to improve black truffle productivity, developing agronomic practices such as 'truffle nests' (peat amendments that are supplemented with truffle spore inoculum). It has been hypothesized that improved fruiting associated with nests is linked to stimulation of truffle mycelia previously established in soil or to changes generated in soil fungal community. To assess this, we used real-time PCR to quantify black truffle extraradical mycelium during 2 years after nests installation. We also characterized the fungal community via high-throughput amplicon sequencing of the ITS region of rRNA genes. We found that neither the abundance of truffle mycelium in nests nor in the soil-nest interphase was higher than in the bulk soil, which indicates that nests do not improve mycelial growth. The fungal community in nests showed lower richness and Shannon index and was compositionally different from that of soil, which suggests that nests may act as an open niche for fungal colonization that facilitates truffle fruiting. The ectomycorrhizal fungal community showed lower richness in nests. However, no negative relationships between amount of truffle mycelium and reads of other ectomycorrhizal fungi were found, thus countering the hypothesis that ectomycorrhizal competition plays a role in the nest effect. This work was supported by the Instituto Nacional de Investigaciones Agrarias (collaboration agreement 2016/1/0010 and RTA2015-00053-00-00 to S. S.); Spanish Ministry of Science, Innovation and Universities (RTI2018-093907-B-C21/22 to J. P.), and the National Science Foundation (Award #1946445 to G. B.). Peer reviewed 2023-09-12T08:04:14Z 2023-09-12T08:04:14Z 2023-08-01 artículo http://purl.org/coar/resource_type/c_6501 FEMS Microbiology Ecology 99(8): fiad084 (2023) 0168-6496 http://hdl.handle.net/10261/334909 10.1093/femsec/fiad084 1574-6941 http://dx.doi.org/10.13039/100000001 37481697 2-s2.0-85166376613 https://api.elsevier.com/content/abstract/scopus_id/85166376613 en #PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/INIA-CSIC//RTA2015-00053-00-00 info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-093907-B-C21/ES/DISEÑO E IMPLEMENTACION DE SISTEMAS MULTIPRODUCTIVOS SOSTENIBLES EN TRUFICULTURA Y ANALISIS DE LOS SERVICIOS ECOSISTEMICOS ASOCIADOS/ info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-093907-B-C22/ES/DISEÑO E IMPLEMENTACION DE SISTEMAS MULTIPRODUCTIVOS SOSTENIBLES EN TRUFICULTURA Y ANALISIS DE LOS SERVICIOS ECOSISTEMICOS ASOCIADOS/ Departamento de Protección Vegetal Publisher's version https://doi.org/10.1093/femsec/fiad084 Sí open Oxford University Press |
institution |
INIA ES |
collection |
DSpace |
country |
España |
countrycode |
ES |
component |
Bibliográfico |
access |
En linea |
databasecode |
dig-inia-es |
tag |
biblioteca |
region |
Europa del Sur |
libraryname |
Biblioteca del INIA España |
language |
English |
topic |
Tuber melanosporum ITS amplicon sequencing Ectomycorrhizal fungi Extraradical mycelium Fungal community Truffle nests Strengthen the means of implementation and revitalize the Global Partnership for Sustainable Development Tuber melanosporum ITS amplicon sequencing Ectomycorrhizal fungi Extraradical mycelium Fungal community Truffle nests Strengthen the means of implementation and revitalize the Global Partnership for Sustainable Development |
spellingShingle |
Tuber melanosporum ITS amplicon sequencing Ectomycorrhizal fungi Extraradical mycelium Fungal community Truffle nests Strengthen the means of implementation and revitalize the Global Partnership for Sustainable Development Tuber melanosporum ITS amplicon sequencing Ectomycorrhizal fungi Extraradical mycelium Fungal community Truffle nests Strengthen the means of implementation and revitalize the Global Partnership for Sustainable Development Garcia-Barreda, Sergi Marco, Pedro Bonito, Gregory Parladé, Javier Sánchez, Sergio González, Vicente Larena, Inmaculada Benucci, Gian Maria Niccolò Interannual dynamics of Tuber melanosporum and fungal communities in productive black truffle orchards amended with truffle nests |
description |
Truffle growers devote great efforts to improve black truffle productivity, developing agronomic practices such as 'truffle nests' (peat amendments that are supplemented with truffle spore inoculum). It has been hypothesized that improved fruiting associated with nests is linked to stimulation of truffle mycelia previously established in soil or to changes generated in soil fungal community. To assess this, we used real-time PCR to quantify black truffle extraradical mycelium during 2 years after nests installation. We also characterized the fungal community via high-throughput amplicon sequencing of the ITS region of rRNA genes. We found that neither the abundance of truffle mycelium in nests nor in the soil-nest interphase was higher than in the bulk soil, which indicates that nests do not improve mycelial growth. The fungal community in nests showed lower richness and Shannon index and was compositionally different from that of soil, which suggests that nests may act as an open niche for fungal colonization that facilitates truffle fruiting. The ectomycorrhizal fungal community showed lower richness in nests. However, no negative relationships between amount of truffle mycelium and reads of other ectomycorrhizal fungi were found, thus countering the hypothesis that ectomycorrhizal competition plays a role in the nest effect. |
author2 |
CSIC - Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA) |
author_facet |
CSIC - Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA) Garcia-Barreda, Sergi Marco, Pedro Bonito, Gregory Parladé, Javier Sánchez, Sergio González, Vicente Larena, Inmaculada Benucci, Gian Maria Niccolò |
format |
artículo |
topic_facet |
Tuber melanosporum ITS amplicon sequencing Ectomycorrhizal fungi Extraradical mycelium Fungal community Truffle nests Strengthen the means of implementation and revitalize the Global Partnership for Sustainable Development |
author |
Garcia-Barreda, Sergi Marco, Pedro Bonito, Gregory Parladé, Javier Sánchez, Sergio González, Vicente Larena, Inmaculada Benucci, Gian Maria Niccolò |
author_sort |
Garcia-Barreda, Sergi |
title |
Interannual dynamics of Tuber melanosporum and fungal communities in productive black truffle orchards amended with truffle nests |
title_short |
Interannual dynamics of Tuber melanosporum and fungal communities in productive black truffle orchards amended with truffle nests |
title_full |
Interannual dynamics of Tuber melanosporum and fungal communities in productive black truffle orchards amended with truffle nests |
title_fullStr |
Interannual dynamics of Tuber melanosporum and fungal communities in productive black truffle orchards amended with truffle nests |
title_full_unstemmed |
Interannual dynamics of Tuber melanosporum and fungal communities in productive black truffle orchards amended with truffle nests |
title_sort |
interannual dynamics of tuber melanosporum and fungal communities in productive black truffle orchards amended with truffle nests |
publisher |
Oxford University Press |
publishDate |
2023-08-01 |
url |
http://hdl.handle.net/10261/334909 http://dx.doi.org/10.13039/100000001 https://api.elsevier.com/content/abstract/scopus_id/85166376613 |
work_keys_str_mv |
AT garciabarredasergi interannualdynamicsoftubermelanosporumandfungalcommunitiesinproductiveblacktruffleorchardsamendedwithtrufflenests AT marcopedro interannualdynamicsoftubermelanosporumandfungalcommunitiesinproductiveblacktruffleorchardsamendedwithtrufflenests AT bonitogregory interannualdynamicsoftubermelanosporumandfungalcommunitiesinproductiveblacktruffleorchardsamendedwithtrufflenests AT parladejavier interannualdynamicsoftubermelanosporumandfungalcommunitiesinproductiveblacktruffleorchardsamendedwithtrufflenests AT sanchezsergio interannualdynamicsoftubermelanosporumandfungalcommunitiesinproductiveblacktruffleorchardsamendedwithtrufflenests AT gonzalezvicente interannualdynamicsoftubermelanosporumandfungalcommunitiesinproductiveblacktruffleorchardsamendedwithtrufflenests AT larenainmaculada interannualdynamicsoftubermelanosporumandfungalcommunitiesinproductiveblacktruffleorchardsamendedwithtrufflenests AT benuccigianmarianiccolo interannualdynamicsoftubermelanosporumandfungalcommunitiesinproductiveblacktruffleorchardsamendedwithtrufflenests |
_version_ |
1802819782631751680 |