Multiplex Assay for Simultaneous Detection of Antibodies against Crimean-Congo Hemorrhagic Fever Virus Nucleocapsid Protein and Glycoproteins in Ruminants
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| Format: | artículo biblioteca |
| Language: | English |
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American Society for Microbiology
2023-02-23
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| Subjects: | CCHFV, Crimean-Congo hemorrhagic fever virus, Diagnosis, Glycoproteins, Multiplex assay, Nucleocapsid protein, |
| Online Access: | http://hdl.handle.net/10261/330114 http://dx.doi.org/10.13039/501100000780 https://api.elsevier.com/content/abstract/scopus_id/85153856061 |
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CCHFV Crimean-Congo hemorrhagic fever virus Diagnosis Glycoproteins Multiplex assay Nucleocapsid protein CCHFV Crimean-Congo hemorrhagic fever virus Diagnosis Glycoproteins Multiplex assay Nucleocapsid protein |
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CCHFV Crimean-Congo hemorrhagic fever virus Diagnosis Glycoproteins Multiplex assay Nucleocapsid protein CCHFV Crimean-Congo hemorrhagic fever virus Diagnosis Glycoproteins Multiplex assay Nucleocapsid protein Hoste, Alexis C.R. Djadjovski, Igor Jiménez-Clavero, Miguel Ángel Rueda, Paloma Barr, John N. Sastre, Patricia Multiplex Assay for Simultaneous Detection of Antibodies against Crimean-Congo Hemorrhagic Fever Virus Nucleocapsid Protein and Glycoproteins in Ruminants |
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10 Pág. |
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European Commission |
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European Commission Hoste, Alexis C.R. Djadjovski, Igor Jiménez-Clavero, Miguel Ángel Rueda, Paloma Barr, John N. Sastre, Patricia |
| format |
artículo |
| topic_facet |
CCHFV Crimean-Congo hemorrhagic fever virus Diagnosis Glycoproteins Multiplex assay Nucleocapsid protein |
| author |
Hoste, Alexis C.R. Djadjovski, Igor Jiménez-Clavero, Miguel Ángel Rueda, Paloma Barr, John N. Sastre, Patricia |
| author_sort |
Hoste, Alexis C.R. |
| title |
Multiplex Assay for Simultaneous Detection of Antibodies against Crimean-Congo Hemorrhagic Fever Virus Nucleocapsid Protein and Glycoproteins in Ruminants |
| title_short |
Multiplex Assay for Simultaneous Detection of Antibodies against Crimean-Congo Hemorrhagic Fever Virus Nucleocapsid Protein and Glycoproteins in Ruminants |
| title_full |
Multiplex Assay for Simultaneous Detection of Antibodies against Crimean-Congo Hemorrhagic Fever Virus Nucleocapsid Protein and Glycoproteins in Ruminants |
| title_fullStr |
Multiplex Assay for Simultaneous Detection of Antibodies against Crimean-Congo Hemorrhagic Fever Virus Nucleocapsid Protein and Glycoproteins in Ruminants |
| title_full_unstemmed |
Multiplex Assay for Simultaneous Detection of Antibodies against Crimean-Congo Hemorrhagic Fever Virus Nucleocapsid Protein and Glycoproteins in Ruminants |
| title_sort |
multiplex assay for simultaneous detection of antibodies against crimean-congo hemorrhagic fever virus nucleocapsid protein and glycoproteins in ruminants |
| publisher |
American Society for Microbiology |
| publishDate |
2023-02-23 |
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http://hdl.handle.net/10261/330114 http://dx.doi.org/10.13039/501100000780 https://api.elsevier.com/content/abstract/scopus_id/85153856061 |
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1816136432166633472 |
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dig-inia-es-10261-3301142024-10-27T21:36:21Z Multiplex Assay for Simultaneous Detection of Antibodies against Crimean-Congo Hemorrhagic Fever Virus Nucleocapsid Protein and Glycoproteins in Ruminants Hoste, Alexis C.R. Djadjovski, Igor Jiménez-Clavero, Miguel Ángel Rueda, Paloma Barr, John N. Sastre, Patricia European Commission Hoste, Alexis C.R. [0000-0001-6724-6062] Djadjovski, Igor [0000-0002-4820-3590] Jiménez-Clavero, Miguel Ángel [0000-0003-2125-9743] Barr, John N. [0000-0002-9035-2039] Sastre, Patricia [0000-0003-3732-5047] Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72] CCHFV Crimean-Congo hemorrhagic fever virus Diagnosis Glycoproteins Multiplex assay Nucleocapsid protein 10 Pág. Crimean-Congo hemorrhagic fever virus (CCHFV) is a widespread tick-borne zoonotic virus that causes Crimean-Congo hemorrhagic fever (CCHF). CCHF is asymptomatic in infected animals but can develop into severe illness in humans, with high case-fatality rates. Due to complex environmental and socio-economic factors, the distribution of CCHFV vectors is changing, leading to disease occurrence in previously unaffected countries. Neither an effective treatment nor a vaccine has been developed against CCHFV; thus, surveillance programs are essential to limit and control the spread of the virus. Furthermore, the WHO highlighted the need of assays that can cover a range of CCHFV antigenic targets, DIVA (differentiating infected from vaccinated animals) assays, or assays for future vaccine evaluation. Here, we developed a multiplex assay, based on a suspension microarray, able to detect specific antibodies in ruminants to three recombinantly produced CCHFV proteins: the nucleocapsid (N) protein and two glycoproteins, GN ectodomain (GNe), and GP38. This triplex assay was used to assess the antibody response in naturally infected animals. Out of the 29 positive field sera to the N protein, 40% showed antibodies against GNe or GP38, with 11 out of these 12 samples being positive to both glycoproteins. To determine the diagnostic specificity of the test, a total of 147 sera from Spanish farms free of CCHFV were included in the study. This multiplex assay could be useful to detect antibodies to different proteins of CCHFV as vaccine target candidates and to study the immune response to CCHFV in infected animals and for surveillance programs to prevent the further spread of the virus. IMPORTANCE Crimean-Congo hemorrhagic fever virus (CCHFV) causes Crimean-Congo hemorrhagic fever, which is one of the most important tick-borne viral diseases of humans and has recently been found in previously unaffected countries such as Spain. The disease is asymptomatic in infected animals but can develop into severe illness in humans. As neither an effective treatment nor a vaccine has been developed against CCHFV, surveillance programs are essential to limit and control the spread of the virus. In this study, a multiplex assay detecting antibodies against different CCHFV antigens in a single sample and independent of the ruminant species has been developed. This assay could be very useful in surveillance studies, to control the spread of CCHFV and prevent future outbreaks, and to better understand the immune response induced by CCHFV. We thank the EU Marie Skłodowska-Curie Actions (MSCA) Innovative Training Network (ITN): H2020-MSCA-ITN-2016, under grant no. 721367 (to Alexis C. R. Hoste) and the MediLabSecure Project, supported by the European Commission (DEVCO: IFS/2018/402-247) (to Igor Djadjovski and Miguel Ángel Jiménez-Clavero). Peer reviewed 2023-06-30T10:10:48Z 2023-06-30T10:10:48Z 2023-02-23 artículo http://purl.org/coar/resource_type/c_6501 Microbiology Spectrum 11(2): e02600-22 (2023) 2165-0497 http://hdl.handle.net/10261/330114 10.1128/spectrum.02600-22 http://dx.doi.org/10.13039/501100000780 36815788 2-s2.0-85153856061 https://api.elsevier.com/content/abstract/scopus_id/85153856061 en #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/EC/H2020/721367 Centro de Investigación en Sanidad Animal (CISA) Publisher's version https://doi.org/10.1128/spectrum.02600-22 Sí open application/pdf American Society for Microbiology |