Asynchrony between the early embryo and the reproductive tract affects subsequent embryo development in cattle
The aim of this study was to determine the effect of maternal-embryonic asynchrony in the reproductive tract (oviduct and uterus) on subsequent embryo development in cattle. Fifty Day 1 in vitro-produced zygotes were transferred endoscopically into the oviduct ipsilateral to the corpus luteum of heifers (n = 40) that were either synchronous with the embryos (Day 1 after ovulation) or asynchronous and ahead of the embryo (Day 3 after ovulation). A subset of heifers was killed in a commercial abattoir 3, 6 or 14 days after embryo transfer. Location within the reproductive tract, developmental stage and the quality of embryos were recorded. Transfer of embryos to an advanced (asynchronous) oviduct resulted, on Day 4, in fewer embryos at the expected location (oviduct), and a greater number of degenerated and retarded embryos with a lower total cell number than for embryos in the synchronous group. Similarly, on Day 7, asynchrony led to a greater number of degenerated and retarded embryos compared with the synchronous group. Total embryo cell number was similar among groups. Although Day 15 conceptuses were longer following asynchronous transfer, only 50% of the asynchronous heifers yielded conceptuses, compared with 100% in the synchronous group. In conclusion, asynchrony between the developing embryo and the reproductive tract has a negative effect on embryo development.
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CSIRO Publishing
2020-01-29
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Subjects: | Bovine, Mammalia, Preimplantation, Reproduction, |
Online Access: | http://hdl.handle.net/10261/329890 http://dx.doi.org/10.13039/501100001602 http://dx.doi.org/10.13039/501100000780 https://api.elsevier.com/content/abstract/scopus_id/85078745566 |
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dig-inia-es-10261-3298902024-05-14T20:41:32Z Asynchrony between the early embryo and the reproductive tract affects subsequent embryo development in cattle Rodríguez-Alonso, Beatriz Sánchez, José María Hamdi, M. McDonald, Michael Havlicek, V. Besenfelder, U. Lonergan, P. Rizos, Dimitrios European Commission Ministerio de Ciencia, Innovación y Universidades (España) Science Foundation Ireland Fernández Sánchez, José María [0000-0003-3889-2342] Hamdi, M. [0000-0002-2792-8293] Havlicek, V. [0009-0008-8271-0001] Besenfelder, U. [0009-0008-9443-1130] Lonergan, P. [0000-0001-9500-057X] Rizos, Dimitrios [0000-0001-6813-3940] Bovine Mammalia Preimplantation Reproduction The aim of this study was to determine the effect of maternal-embryonic asynchrony in the reproductive tract (oviduct and uterus) on subsequent embryo development in cattle. Fifty Day 1 in vitro-produced zygotes were transferred endoscopically into the oviduct ipsilateral to the corpus luteum of heifers (n = 40) that were either synchronous with the embryos (Day 1 after ovulation) or asynchronous and ahead of the embryo (Day 3 after ovulation). A subset of heifers was killed in a commercial abattoir 3, 6 or 14 days after embryo transfer. Location within the reproductive tract, developmental stage and the quality of embryos were recorded. Transfer of embryos to an advanced (asynchronous) oviduct resulted, on Day 4, in fewer embryos at the expected location (oviduct), and a greater number of degenerated and retarded embryos with a lower total cell number than for embryos in the synchronous group. Similarly, on Day 7, asynchrony led to a greater number of degenerated and retarded embryos compared with the synchronous group. Total embryo cell number was similar among groups. Although Day 15 conceptuses were longer following asynchronous transfer, only 50% of the asynchronous heifers yielded conceptuses, compared with 100% in the synchronous group. In conclusion, asynchrony between the developing embryo and the reproductive tract has a negative effect on embryo development. This study was supported by the European Union H2020 Marie Sklodowska Curie (MSCA) Innovative raining Network (ITN) project ‘Biology and Technology of Reproductive Health – REP-BIOTECH – 675526’, the spanish Ministry of Science, Innovation and Universities – AGL2015-70140-R and Science Foundation Ireland (Grant: 13/IA/1983). Peer reviewed 2023-06-28T09:59:32Z 2023-06-28T09:59:32Z 2020-01-29 artículo http://purl.org/coar/resource_type/c_6501 Reproduction, Fertility and Development 32(6): 564-571 (2020) 1031-3613 http://hdl.handle.net/10261/329890 10.1071/RD19360 1448-5990 http://dx.doi.org/10.13039/501100001602 http://dx.doi.org/10.13039/501100000780 32209207 2-s2.0-85078745566 https://api.elsevier.com/content/abstract/scopus_id/85078745566 en #PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/EC/H2020/675526 info:eu-repo/grantAgreement/MINECO//AGL2015-70140-R/ES/INTERACCION EMBRIO-MATERNAL: BALANCE FUNDAMENTAL PARA EL EXITO REPRODUCTIVO EN EL GANADO BOVINO/ Departamento de Reproducción Animal Publisher's version https://doi.org/10.1071/RD19360 Sí open CSIRO Publishing |
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Bovine Mammalia Preimplantation Reproduction Bovine Mammalia Preimplantation Reproduction Rodríguez-Alonso, Beatriz Sánchez, José María Hamdi, M. McDonald, Michael Havlicek, V. Besenfelder, U. Lonergan, P. Rizos, Dimitrios Asynchrony between the early embryo and the reproductive tract affects subsequent embryo development in cattle |
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The aim of this study was to determine the effect of maternal-embryonic asynchrony in the reproductive tract (oviduct and uterus) on subsequent embryo development in cattle. Fifty Day 1 in vitro-produced zygotes were transferred endoscopically into the oviduct ipsilateral to the corpus luteum of heifers (n = 40) that were either synchronous with the embryos (Day 1 after ovulation) or asynchronous and ahead of the embryo (Day 3 after ovulation). A subset of heifers was killed in a commercial abattoir 3, 6 or 14 days after embryo transfer. Location within the reproductive tract, developmental stage and the quality of embryos were recorded. Transfer of embryos to an advanced (asynchronous) oviduct resulted, on Day 4, in fewer embryos at the expected location (oviduct), and a greater number of degenerated and retarded embryos with a lower total cell number than for embryos in the synchronous group. Similarly, on Day 7, asynchrony led to a greater number of degenerated and retarded embryos compared with the synchronous group. Total embryo cell number was similar among groups. Although Day 15 conceptuses were longer following asynchronous transfer, only 50% of the asynchronous heifers yielded conceptuses, compared with 100% in the synchronous group. In conclusion, asynchrony between the developing embryo and the reproductive tract has a negative effect on embryo development. |
author2 |
European Commission |
author_facet |
European Commission Rodríguez-Alonso, Beatriz Sánchez, José María Hamdi, M. McDonald, Michael Havlicek, V. Besenfelder, U. Lonergan, P. Rizos, Dimitrios |
format |
artículo |
topic_facet |
Bovine Mammalia Preimplantation Reproduction |
author |
Rodríguez-Alonso, Beatriz Sánchez, José María Hamdi, M. McDonald, Michael Havlicek, V. Besenfelder, U. Lonergan, P. Rizos, Dimitrios |
author_sort |
Rodríguez-Alonso, Beatriz |
title |
Asynchrony between the early embryo and the reproductive tract affects subsequent embryo development in cattle |
title_short |
Asynchrony between the early embryo and the reproductive tract affects subsequent embryo development in cattle |
title_full |
Asynchrony between the early embryo and the reproductive tract affects subsequent embryo development in cattle |
title_fullStr |
Asynchrony between the early embryo and the reproductive tract affects subsequent embryo development in cattle |
title_full_unstemmed |
Asynchrony between the early embryo and the reproductive tract affects subsequent embryo development in cattle |
title_sort |
asynchrony between the early embryo and the reproductive tract affects subsequent embryo development in cattle |
publisher |
CSIRO Publishing |
publishDate |
2020-01-29 |
url |
http://hdl.handle.net/10261/329890 http://dx.doi.org/10.13039/501100001602 http://dx.doi.org/10.13039/501100000780 https://api.elsevier.com/content/abstract/scopus_id/85078745566 |
work_keys_str_mv |
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1802819757435518976 |