Immunization with a small fragment of the Schmallenberg virus nucleoprotein highly conserved across the Orthobunyaviruses of the Simbu serogroup reduces viremia in SBV challenged IFNAR<sup>-/-</sup> mice
10 Pág.
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Other Authors: | |
| Format: | artículo biblioteca |
| Language: | English |
| Published: |
Elsevier
2023-05-11
|
| Subjects: | Cell-mediate immunity, Orthobunyavirus, Pan-vaccine, Schmallenberg virus, Subunit vaccine, |
| Online Access: | http://hdl.handle.net/10261/329733 http://dx.doi.org/10.13039/501100011033 https://api.elsevier.com/content/abstract/scopus_id/85153098116 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| id |
dig-inia-es-10261-329733 |
|---|---|
| record_format |
koha |
| institution |
INIA ES |
| collection |
DSpace |
| country |
España |
| countrycode |
ES |
| component |
Bibliográfico |
| access |
En linea |
| databasecode |
dig-inia-es |
| tag |
biblioteca |
| region |
Europa del Sur |
| libraryname |
Biblioteca del INIA España |
| language |
English |
| topic |
Cell-mediate immunity Orthobunyavirus Pan-vaccine Schmallenberg virus Subunit vaccine Cell-mediate immunity Orthobunyavirus Pan-vaccine Schmallenberg virus Subunit vaccine |
| spellingShingle |
Cell-mediate immunity Orthobunyavirus Pan-vaccine Schmallenberg virus Subunit vaccine Cell-mediate immunity Orthobunyavirus Pan-vaccine Schmallenberg virus Subunit vaccine Guerra, Gabriel Soares Barriales, Diego Lorenzo, Gema Moreno, Sandra Anguita, Juan Brun Torres, Alejandro Abrescia, Nicola G.A. Immunization with a small fragment of the Schmallenberg virus nucleoprotein highly conserved across the Orthobunyaviruses of the Simbu serogroup reduces viremia in SBV challenged IFNAR<sup>-/-</sup> mice |
| description |
10 Pág. |
| author2 |
Ministerio de Ciencia, Innovación y Universidades (España) |
| author_facet |
Ministerio de Ciencia, Innovación y Universidades (España) Guerra, Gabriel Soares Barriales, Diego Lorenzo, Gema Moreno, Sandra Anguita, Juan Brun Torres, Alejandro Abrescia, Nicola G.A. |
| format |
artículo |
| topic_facet |
Cell-mediate immunity Orthobunyavirus Pan-vaccine Schmallenberg virus Subunit vaccine |
| author |
Guerra, Gabriel Soares Barriales, Diego Lorenzo, Gema Moreno, Sandra Anguita, Juan Brun Torres, Alejandro Abrescia, Nicola G.A. |
| author_sort |
Guerra, Gabriel Soares |
| title |
Immunization with a small fragment of the Schmallenberg virus nucleoprotein highly conserved across the Orthobunyaviruses of the Simbu serogroup reduces viremia in SBV challenged IFNAR<sup>-/-</sup> mice |
| title_short |
Immunization with a small fragment of the Schmallenberg virus nucleoprotein highly conserved across the Orthobunyaviruses of the Simbu serogroup reduces viremia in SBV challenged IFNAR<sup>-/-</sup> mice |
| title_full |
Immunization with a small fragment of the Schmallenberg virus nucleoprotein highly conserved across the Orthobunyaviruses of the Simbu serogroup reduces viremia in SBV challenged IFNAR<sup>-/-</sup> mice |
| title_fullStr |
Immunization with a small fragment of the Schmallenberg virus nucleoprotein highly conserved across the Orthobunyaviruses of the Simbu serogroup reduces viremia in SBV challenged IFNAR<sup>-/-</sup> mice |
| title_full_unstemmed |
Immunization with a small fragment of the Schmallenberg virus nucleoprotein highly conserved across the Orthobunyaviruses of the Simbu serogroup reduces viremia in SBV challenged IFNAR<sup>-/-</sup> mice |
| title_sort |
immunization with a small fragment of the schmallenberg virus nucleoprotein highly conserved across the orthobunyaviruses of the simbu serogroup reduces viremia in sbv challenged ifnar<sup>-/-</sup> mice |
| publisher |
Elsevier |
| publishDate |
2023-05-11 |
| url |
http://hdl.handle.net/10261/329733 http://dx.doi.org/10.13039/501100011033 https://api.elsevier.com/content/abstract/scopus_id/85153098116 |
| work_keys_str_mv |
AT guerragabrielsoares immunizationwithasmallfragmentoftheschmallenbergvirusnucleoproteinhighlyconservedacrosstheorthobunyavirusesofthesimbuserogroupreducesviremiainsbvchallengedifnarsupsupmice AT barrialesdiego immunizationwithasmallfragmentoftheschmallenbergvirusnucleoproteinhighlyconservedacrosstheorthobunyavirusesofthesimbuserogroupreducesviremiainsbvchallengedifnarsupsupmice AT lorenzogema immunizationwithasmallfragmentoftheschmallenbergvirusnucleoproteinhighlyconservedacrosstheorthobunyavirusesofthesimbuserogroupreducesviremiainsbvchallengedifnarsupsupmice AT morenosandra immunizationwithasmallfragmentoftheschmallenbergvirusnucleoproteinhighlyconservedacrosstheorthobunyavirusesofthesimbuserogroupreducesviremiainsbvchallengedifnarsupsupmice AT anguitajuan immunizationwithasmallfragmentoftheschmallenbergvirusnucleoproteinhighlyconservedacrosstheorthobunyavirusesofthesimbuserogroupreducesviremiainsbvchallengedifnarsupsupmice AT bruntorresalejandro immunizationwithasmallfragmentoftheschmallenbergvirusnucleoproteinhighlyconservedacrosstheorthobunyavirusesofthesimbuserogroupreducesviremiainsbvchallengedifnarsupsupmice AT abrescianicolaga immunizationwithasmallfragmentoftheschmallenbergvirusnucleoproteinhighlyconservedacrosstheorthobunyavirusesofthesimbuserogroupreducesviremiainsbvchallengedifnarsupsupmice |
| _version_ |
1816136425419046912 |
| spelling |
dig-inia-es-10261-3297332024-10-31T21:35:47Z Immunization with a small fragment of the Schmallenberg virus nucleoprotein highly conserved across the Orthobunyaviruses of the Simbu serogroup reduces viremia in SBV challenged IFNAR<sup>-/-</sup> mice Guerra, Gabriel Soares Barriales, Diego Lorenzo, Gema Moreno, Sandra Anguita, Juan Brun Torres, Alejandro Abrescia, Nicola G.A. Ministerio de Ciencia, Innovación y Universidades (España) Agencia Estatal de Investigación (España) Fundación la Caixa Guerra, Gabriel Soares [0000-0002-5277-5938] Barriales, Diego [0000-0002-6433-3379] Lorenzo, Gema [0000-0003-1869-9051] Moreno, Sandra [0000-0002-3548-037X] Anguita, Juan [0000-0003-2061-7182] Brun Torres, Alejandro [0000-0001-7865-538X] Abrescia, Nicola G.A. [0000-0001-5559-1918] Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72] Cell-mediate immunity Orthobunyavirus Pan-vaccine Schmallenberg virus Subunit vaccine 10 Pág. Schmallenberg Virus (SBV), an arbovirus from the Peribunyaviridae family and Orthobunyavirus genus, was discovered in late 2011 in Germany and has been circulating in Europe, Asia and Africa ever since. The virus causes a disease associated with ruminants that includes fever, fetal malformation, drop in milk production, diarrhoea and stillbirths, becoming a burden for small and large farms. Building on previous studies on SBV nucleoprotein (SBV-N) as a promising vaccine candidate, we have investigated the possible protein regions responsible for protection. Based on selective truncation of domains designed from the available crystal structure of the SBV-N, we identified both the N-terminal domain (N-term; Met1 – Thr133) and a smaller fragment within (C4; Met1 – Ala58) as vaccine prototypes. Two injections of the N-term and C4 polypeptides protected mice knockout for type I interferon (IFN) receptors (IFNAR-/-) challenged with virulent SBV, opposite to control groups that presented severe signs of morbidity and weight loss. Viremia analyses along with the presence of IFN-γ secreted from splenocytes re-stimulated with the N-terminal region of the protein corroborate that these two portions of SBV-N can be employed as subunit vaccines. Apart from both proteinaceous fragments being easily produced in bacterial cells, the C4 polypeptide shares a high sequence homology (∼87.1 %) with the corresponding region of nucleoproteins of several viruses of the Simbu serogroup, a group of Orthobunyaviruses that comprises SBV and veterinary pathogens like Akabane virus and human infecting viruses like Oropouche. Thus, we propose that this smaller fragment is better suited for vaccine nanoparticle formulation, and it paves the way to further research with other related Orthobunyaviruses. This work was supported by the Spanish Ministerio de Ciencia, Innovación y Universidades RTI2018-095700-B-I00 (NGAA), RTI2018-096494-B-I00 (JA), AGL2017-83226 (AB) and “La Caixa” Foundation (ID 100010434) INPhINIT “La Caixa” fellowship LCF/BQ/DI19/11730041 (GSG and NGAA). Peer reviewed 2023-06-27T08:30:28Z 2023-06-27T08:30:28Z 2023-05-11 artículo http://purl.org/coar/resource_type/c_6501 Vaccine 41(20): 3275-3284 (2023) 0264-410X http://hdl.handle.net/10261/329733 10.1016/j.vaccine.2023.04.027 http://dx.doi.org/10.13039/501100011033 37085455 2-s2.0-85153098116 https://api.elsevier.com/content/abstract/scopus_id/85153098116 en #PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-095700-B-I00/ES/MEJORA DE LA SALUD HUMANA Y ANIMAL A TRAVES DE ESTUDIOS ESTRUCTURALES Y FUNCIONALES DE LAS INTERACCIONES VIRUS-HUESPED Y ENSEMBLAJE VIRAL/ info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-096494-B-I00/ES/DINAMICA DE LA RESPUESTA DE MACROFAGOS A PATOGENOS Y SIMBIONTES/ info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2017-83226-R/ES/UTILIZACION DEL VIRUS DE LA FIEBRE DEL VALLE DEL RIFT COMO VECTOR VACUNAL: MEJORA RACIONAL DE SU SEGURIDAD, ESTABILIDAD Y TRAZABILIDAD/ Centro de Investigación en Sanidad Animal (CISA) https://doi.org/10.1016/j.vaccine.2023.04.027 Sí open application/pdf Elsevier |