Identification of Vitis vinifera L. Local Cultivars Recovered in Andalusia (Spain) by Using Microsatellite Markers
11 Pàg.
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Multidisciplinary Digital Publishing Institute
2023-02-28
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Subjects: | Genetic characterization, Grapevine, Synonymies, |
Online Access: | http://hdl.handle.net/10261/329706 http://dx.doi.org/10.13039/501100006280 https://api.elsevier.com/content/abstract/scopus_id/85151466228 |
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dig-inia-es-10261-3297062024-05-20T20:46:19Z Identification of Vitis vinifera L. Local Cultivars Recovered in Andalusia (Spain) by Using Microsatellite Markers Jiménez-Cantizano, A. Puig-Pujol, Anna Arroyo García, Rosa Adela Ministerio de Ciencia y Tecnología (España) Jiménez-Cantizano, A. [0000-0002-3779-3214] Puig-Pujol, Anna [0000-0001-5041-6432] Arroyo García, Rosa Adela [0000-0002-0597-1282] Genetic characterization Grapevine Synonymies 11 Pàg. In Andalusia (Spain), there are different wine regions that have a great recognized tradition. In these regions, the cultivation of the vine is ancient and there are still vineyards planted with local varieties of Vitis vinifera L. that have not yet been identified. The aim of this research study was to identify 49 accessions of grapevine collected in the districts of four provinces in Andalusia (Spain). All samples were genotyped with 20 microsatellite markers in order to ascertain the identity and analyze the genetic diversity of the collected material. In total, 30 different genotypes were obtained, 22 of them which were identified with named, known varieties by comparison to the Spanish or European microsatellite databases, and eight which are referred to as new genotypes. All loci were polymorphic, and a total of 159 alleles were detected, ranging from 4 to 12 alleles per locus, with an average allele number of 7.95. The overall observed heterozygosity was 0.763 and was slightly higher than expected (0.715), while the gene diversity per locus varied between 0.167 (VVIN73) and 0.967 (VVMD5). A dendrogram representing the genetic similarities among cultivars was depicted using the UPGMA method to investigate their relationships. The eight new genotypes identified in this research work could represent ancient local varieties in danger of extinction. These new cultivars may be used to determine original wines. This research was funded by Ministerio de Ciencia y Tecnología (INIA-Spain), grants number RF2004-00014-00-00, VIN00-036-C6-5X, RF2006-00011-00-00 and RF2007-00017-00-00. Peer reviewed 2023-06-27T07:01:12Z 2023-06-27T07:01:12Z 2023-02-28 artículo http://purl.org/coar/resource_type/c_6501 Horticulturae 9(3): e316 (2023) http://hdl.handle.net/10261/329706 10.3390/horticulturae9030316 2311-7524 http://dx.doi.org/10.13039/501100006280 2-s2.0-85151466228 https://api.elsevier.com/content/abstract/scopus_id/85151466228 en #PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/MCT//RF2004-00014-00-00 info:eu-repo/grantAgreement/MCT//VIN00-036-C6-5X info:eu-repo/grantAgreement/MEC//RF2006-00011-00-00/ES/Caracterización genética de las accesiones de vid de vinificación del banco de germoplasma del Rancho de la Merced/ info:eu-repo/grantAgreement/MEC//RF2007-00017-00-00/ES/Prospección, recogida y multiplicación de variedades minoritarias de vid (Vitis vinifera) en peligro de extinción en la comunidad autónoma andaluza/ Centro de Biotecnología y Genómica de Plantas (CBGP) Publisher's version https://doi.org/10.3390/horticulturae9030316 Sí open application/pdf Multidisciplinary Digital Publishing Institute |
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Genetic characterization Grapevine Synonymies Genetic characterization Grapevine Synonymies |
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Genetic characterization Grapevine Synonymies Genetic characterization Grapevine Synonymies Jiménez-Cantizano, A. Puig-Pujol, Anna Arroyo García, Rosa Adela Identification of Vitis vinifera L. Local Cultivars Recovered in Andalusia (Spain) by Using Microsatellite Markers |
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11 Pàg. |
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Ministerio de Ciencia y Tecnología (España) |
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Ministerio de Ciencia y Tecnología (España) Jiménez-Cantizano, A. Puig-Pujol, Anna Arroyo García, Rosa Adela |
format |
artículo |
topic_facet |
Genetic characterization Grapevine Synonymies |
author |
Jiménez-Cantizano, A. Puig-Pujol, Anna Arroyo García, Rosa Adela |
author_sort |
Jiménez-Cantizano, A. |
title |
Identification of Vitis vinifera L. Local Cultivars Recovered in Andalusia (Spain) by Using Microsatellite Markers |
title_short |
Identification of Vitis vinifera L. Local Cultivars Recovered in Andalusia (Spain) by Using Microsatellite Markers |
title_full |
Identification of Vitis vinifera L. Local Cultivars Recovered in Andalusia (Spain) by Using Microsatellite Markers |
title_fullStr |
Identification of Vitis vinifera L. Local Cultivars Recovered in Andalusia (Spain) by Using Microsatellite Markers |
title_full_unstemmed |
Identification of Vitis vinifera L. Local Cultivars Recovered in Andalusia (Spain) by Using Microsatellite Markers |
title_sort |
identification of vitis vinifera l. local cultivars recovered in andalusia (spain) by using microsatellite markers |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2023-02-28 |
url |
http://hdl.handle.net/10261/329706 http://dx.doi.org/10.13039/501100006280 https://api.elsevier.com/content/abstract/scopus_id/85151466228 |
work_keys_str_mv |
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1802819755537596416 |