A redundant transcription factor network steers spatiotemporal Arabidopsis triterpene synthesis
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2023-05-15
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European Commission Nguyen, Trang Hieu Thiers, Louis Van Moerkercke, Alex Bai, Yuechen Fernández-Calvo, Patricia Minne, Max Depuydt, Thomas Colinas, Maite Verstaen, Kevin Van Isterdael, Gert Nützmann, Hans-Wilhelm Osbourn, Anne Saeys, Yvan De Rybel, Bert Vandepoele, Klaas Ritter, Andrés Goossens, Alain |
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artículo |
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Nguyen, Trang Hieu Thiers, Louis Van Moerkercke, Alex Bai, Yuechen Fernández-Calvo, Patricia Minne, Max Depuydt, Thomas Colinas, Maite Verstaen, Kevin Van Isterdael, Gert Nützmann, Hans-Wilhelm Osbourn, Anne Saeys, Yvan De Rybel, Bert Vandepoele, Klaas Ritter, Andrés Goossens, Alain |
| spellingShingle |
Nguyen, Trang Hieu Thiers, Louis Van Moerkercke, Alex Bai, Yuechen Fernández-Calvo, Patricia Minne, Max Depuydt, Thomas Colinas, Maite Verstaen, Kevin Van Isterdael, Gert Nützmann, Hans-Wilhelm Osbourn, Anne Saeys, Yvan De Rybel, Bert Vandepoele, Klaas Ritter, Andrés Goossens, Alain A redundant transcription factor network steers spatiotemporal Arabidopsis triterpene synthesis |
| author_sort |
Nguyen, Trang Hieu |
| title |
A redundant transcription factor network steers spatiotemporal Arabidopsis triterpene synthesis |
| title_short |
A redundant transcription factor network steers spatiotemporal Arabidopsis triterpene synthesis |
| title_full |
A redundant transcription factor network steers spatiotemporal Arabidopsis triterpene synthesis |
| title_fullStr |
A redundant transcription factor network steers spatiotemporal Arabidopsis triterpene synthesis |
| title_full_unstemmed |
A redundant transcription factor network steers spatiotemporal Arabidopsis triterpene synthesis |
| title_sort |
redundant transcription factor network steers spatiotemporal arabidopsis triterpene synthesis |
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Nature Publishing Group |
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2023-05-15 |
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http://hdl.handle.net/10261/310814 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100004543 http://dx.doi.org/10.13039/501100003130 https://api.elsevier.com/content/abstract/scopus_id/85159345560 |
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dig-inia-es-10261-3108142024-05-16T20:40:19Z A redundant transcription factor network steers spatiotemporal Arabidopsis triterpene synthesis Nguyen, Trang Hieu Thiers, Louis Van Moerkercke, Alex Bai, Yuechen Fernández-Calvo, Patricia Minne, Max Depuydt, Thomas Colinas, Maite Verstaen, Kevin Van Isterdael, Gert Nützmann, Hans-Wilhelm Osbourn, Anne Saeys, Yvan De Rybel, Bert Vandepoele, Klaas Ritter, Andrés Goossens, Alain European Commission Ghent University Flemish Government Research Foundation - Flanders Swiss National Science Foundation China Scholarship Council John Innes Foundation Nguyen, Trang Hieu [0000-0003-1051-8068] Thiers, Louis [0000-0002-8650-508X] Van Moerkercke, Alex [0000-0002-6393-4754] Bai, Yuechen [0000-0002-2314-8660] Fernández-Calvo, Patricia [0000-0002-1576-9651] Minne, Max [0000-0003-3089-2284] Depuydt, Thomas [0000-0001-5889-3962] Colinas, Maite [0000-0001-7053-2983] Verstaen, Kevin [0000-0003-3582-0298] Van Isterdael, Gert [0000-0001-6626-1316] Nützmann, Hans-Wilhelm [0000-0002-6855-7556] Osbourn, Anne [0000-0003-2195-5810] Saeys, Yvan [0000-0002-0415-1506] De Rybel, Bert [0000-0002-9551-042X] Vandepoele, Klaas [0000-0003-4790-2725] Ritter, Andrés [0000-0001-7011-6824] Goossens, Alain [0000-0002-1599-551X] 25 Pág. Plant specialized metabolites modulate developmental and ecological functions and comprise many therapeutic and other high-value compounds. However, the mechanisms determining their cell-specific expression remain unknown. Here we describe the transcriptional regulatory network that underlies cell-specific biosynthesis of triterpenes in Arabidopsis thaliana root tips. Expression of thalianol and marneral biosynthesis pathway genes depends on the phytohormone jasmonate and is limited to outer tissues. We show that this is promoted by the activity of redundant bHLH-type transcription factors from two distinct clades and coactivated by homeodomain factors. Conversely, the DOF-type transcription factor DAG1 and other regulators prevent expression of the triterpene pathway genes in inner tissues. We thus show how precise expression of triterpene biosynthesis genes is determined by a robust network of transactivators, coactivators and counteracting repressors. This Article was written in loving memory of A. Van Moerkercke (1979–2021). The authors thank A. Bleys for critically reading the manuscript; D. Gasperini for kindly sharing the ProMYCs:NLS-VENUS reporter lines, and P. Vittorioso for the dag1 mutant, ProDAG1:GUS and DAG1 over-expressing lines; J. R. Wendrich and T. Eekhout for assistance in the launching and analysis of the scRNAseq experiment; and S. Desmet and G. Goeminne from the VIB Metabolomics Core – Ghent for the thalianol profiling. This work was supported by the European Community’s Seventh Framework Program (FP7/2007–2013) under grant agreement 613692-TriForC and H2020 Program under grant agreement 760331-Newcotiana to A.G.; the Special Research Fund from Ghent University to A.G. and A.R. (project BOF18/GOA/013), and M.M. (project BOF20/GOA/012); the Flemish Government (AI Research program) to Y.S.; the Research Foundation Flanders with research project grants to A.G. (G004515N and G008417N) and a postdoctoral fellowship to P.F.-C.; a Swiss National Science Foundation postdoctoral fellowship (P300PA_177831) to M.C.; and a China Scholarship Council PhD scholarship to Y.B. A.O. acknowledges funding support from the John Innes Foundation and the BBSRC Institute Strategic Program Grant ‘Molecules from Nature – Products and Pathways’ (BBS/E/J/000PR9790). Peer reviewed 2023-06-07T07:52:25Z 2023-06-07T07:52:25Z 2023-05-15 artículo http://purl.org/coar/resource_type/c_6501 Nature Plants : (2023) http://hdl.handle.net/10261/310814 10.1038/s41477-023-01419-8 2055-0278 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100004543 http://dx.doi.org/10.13039/501100003130 37188853 2-s2.0-85159345560 https://api.elsevier.com/content/abstract/scopus_id/85159345560 #PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/EC/613692 info:eu-repo/grantAgreement/EC/H2020/760331 Centro de Biotecnología y Genómica de Plantas (CBGP) https://doi.org/10.1038/s41477-023-01419-8 Sí open Nature Publishing Group |