Influence of Prolactin Secretion Changes on Sperm Head Size and Freezability in Ibex and Mouflon

Aim: This work examined the influence of induced changes in prolactin (PRL) secretion on sperm cryoresistance of ibex and the mouflon. Materials and Methods: PRL secretion was modified in a first experiment by the use of bromocriptine (BCR, dopamine agonist) during the non-breeding season, and in a second experiment by the use of sulpiride (SLP, dopamine D2-receptor antagonist) during the rutting season. Slow and ultra-rapid freezing protocols were used to cryopreserve sperm samples. Results: BCR decreased blood plasma PRL concentrations, whereas SLP increased them. Cryoresistance ratios (CRs) for curvilinear velocity (VCL), straight-line velocity (VSL), and average path velocity (VAP) in BCR-treated mouflons were lower than in controls using slow-freezing (p < 0.05), while CRs of motility and morphologically normal sperm of BCR-treated mouflons were greater than controls with ultra-rapid freezing (p < 0.05). BCR increased the head sperm dimensions in ibexes (p < 0.001); conversely, BCR decreased the head dimensions in mouflons (p < 0.001). CR-motility, CR-amplitude of lateral head displacement (ALH), CR-viability, and CR-acrosome integrity in SLP-treated mouflons were lower than in controls with slow-freezing (p < 0.01); CR-viability and CR-acrosome were lower than controls with ultra-rapid freezing (p < 0.05). In ibexes, CR-ALH was lower for SLP-treated (p < 0.05). SLP treatment increased head dimensions in ibexes (p < 0.001) but did not affect the sperm head of mouflons. Conclusion: Our findings show that high levels of blood plasma PRL negatively affect the cryoresistance of ibex and mouflon sperm.

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Main Authors: Bóveda Gómez, Paula, Velázquez, Rosario, Martínez-Fresneda, Lucía, Mejía, Octavio, Oteo, Marta, Toledano-Díaz, Adolfo, Castaño, Cristina, Esteso, Milagros Cristina, Ungerfeld, Rodolfo, López-Sebastián, Antonio, Santiago-Moreno, Julian
Format: artículo biblioteca
Language:English
Published: 2022-06-08
Subjects:Cryopreservation, Seasonality, Semen, Sperm freezability, Sperm morphometry,
Online Access:http://hdl.handle.net/10261/305117
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spelling dig-inia-es-10261-3051172023-03-30T13:09:02Z Influence of Prolactin Secretion Changes on Sperm Head Size and Freezability in Ibex and Mouflon Bóveda Gómez, Paula Velázquez, Rosario Martínez-Fresneda, Lucía Mejía, Octavio Oteo, Marta Toledano-Díaz, Adolfo Castaño, Cristina Esteso, Milagros Cristina Ungerfeld, Rodolfo López-Sebastián, Antonio Santiago-Moreno, Julian Cryopreservation Seasonality Semen Sperm freezability Sperm morphometry Aim: This work examined the influence of induced changes in prolactin (PRL) secretion on sperm cryoresistance of ibex and the mouflon. Materials and Methods: PRL secretion was modified in a first experiment by the use of bromocriptine (BCR, dopamine agonist) during the non-breeding season, and in a second experiment by the use of sulpiride (SLP, dopamine D2-receptor antagonist) during the rutting season. Slow and ultra-rapid freezing protocols were used to cryopreserve sperm samples. Results: BCR decreased blood plasma PRL concentrations, whereas SLP increased them. Cryoresistance ratios (CRs) for curvilinear velocity (VCL), straight-line velocity (VSL), and average path velocity (VAP) in BCR-treated mouflons were lower than in controls using slow-freezing (p < 0.05), while CRs of motility and morphologically normal sperm of BCR-treated mouflons were greater than controls with ultra-rapid freezing (p < 0.05). BCR increased the head sperm dimensions in ibexes (p < 0.001); conversely, BCR decreased the head dimensions in mouflons (p < 0.001). CR-motility, CR-amplitude of lateral head displacement (ALH), CR-viability, and CR-acrosome integrity in SLP-treated mouflons were lower than in controls with slow-freezing (p < 0.01); CR-viability and CR-acrosome were lower than controls with ultra-rapid freezing (p < 0.05). In ibexes, CR-ALH was lower for SLP-treated (p < 0.05). SLP treatment increased head dimensions in ibexes (p < 0.001) but did not affect the sperm head of mouflons. Conclusion: Our findings show that high levels of blood plasma PRL negatively affect the cryoresistance of ibex and mouflon sperm. Peer reviewed 2023-03-30T13:09:02Z 2023-03-30T13:09:02Z 2022-06-08 artículo http://hdl.handle.net/10261/305117 10.1089/bio.2021.0129 35675676 en Biopreservation and biobanking Sí open
institution INIA ES
collection DSpace
country España
countrycode ES
component Bibliográfico
access En linea
databasecode dig-inia-es
tag biblioteca
region Europa del Sur
libraryname Biblioteca del INIA España
language English
topic Cryopreservation
Seasonality
Semen
Sperm freezability
Sperm morphometry
Cryopreservation
Seasonality
Semen
Sperm freezability
Sperm morphometry
spellingShingle Cryopreservation
Seasonality
Semen
Sperm freezability
Sperm morphometry
Cryopreservation
Seasonality
Semen
Sperm freezability
Sperm morphometry
Bóveda Gómez, Paula
Velázquez, Rosario
Martínez-Fresneda, Lucía
Mejía, Octavio
Oteo, Marta
Toledano-Díaz, Adolfo
Castaño, Cristina
Esteso, Milagros Cristina
Ungerfeld, Rodolfo
López-Sebastián, Antonio
Santiago-Moreno, Julian
Influence of Prolactin Secretion Changes on Sperm Head Size and Freezability in Ibex and Mouflon
description Aim: This work examined the influence of induced changes in prolactin (PRL) secretion on sperm cryoresistance of ibex and the mouflon. Materials and Methods: PRL secretion was modified in a first experiment by the use of bromocriptine (BCR, dopamine agonist) during the non-breeding season, and in a second experiment by the use of sulpiride (SLP, dopamine D2-receptor antagonist) during the rutting season. Slow and ultra-rapid freezing protocols were used to cryopreserve sperm samples. Results: BCR decreased blood plasma PRL concentrations, whereas SLP increased them. Cryoresistance ratios (CRs) for curvilinear velocity (VCL), straight-line velocity (VSL), and average path velocity (VAP) in BCR-treated mouflons were lower than in controls using slow-freezing (p < 0.05), while CRs of motility and morphologically normal sperm of BCR-treated mouflons were greater than controls with ultra-rapid freezing (p < 0.05). BCR increased the head sperm dimensions in ibexes (p < 0.001); conversely, BCR decreased the head dimensions in mouflons (p < 0.001). CR-motility, CR-amplitude of lateral head displacement (ALH), CR-viability, and CR-acrosome integrity in SLP-treated mouflons were lower than in controls with slow-freezing (p < 0.01); CR-viability and CR-acrosome were lower than controls with ultra-rapid freezing (p < 0.05). In ibexes, CR-ALH was lower for SLP-treated (p < 0.05). SLP treatment increased head dimensions in ibexes (p < 0.001) but did not affect the sperm head of mouflons. Conclusion: Our findings show that high levels of blood plasma PRL negatively affect the cryoresistance of ibex and mouflon sperm.
format artículo
topic_facet Cryopreservation
Seasonality
Semen
Sperm freezability
Sperm morphometry
author Bóveda Gómez, Paula
Velázquez, Rosario
Martínez-Fresneda, Lucía
Mejía, Octavio
Oteo, Marta
Toledano-Díaz, Adolfo
Castaño, Cristina
Esteso, Milagros Cristina
Ungerfeld, Rodolfo
López-Sebastián, Antonio
Santiago-Moreno, Julian
author_facet Bóveda Gómez, Paula
Velázquez, Rosario
Martínez-Fresneda, Lucía
Mejía, Octavio
Oteo, Marta
Toledano-Díaz, Adolfo
Castaño, Cristina
Esteso, Milagros Cristina
Ungerfeld, Rodolfo
López-Sebastián, Antonio
Santiago-Moreno, Julian
author_sort Bóveda Gómez, Paula
title Influence of Prolactin Secretion Changes on Sperm Head Size and Freezability in Ibex and Mouflon
title_short Influence of Prolactin Secretion Changes on Sperm Head Size and Freezability in Ibex and Mouflon
title_full Influence of Prolactin Secretion Changes on Sperm Head Size and Freezability in Ibex and Mouflon
title_fullStr Influence of Prolactin Secretion Changes on Sperm Head Size and Freezability in Ibex and Mouflon
title_full_unstemmed Influence of Prolactin Secretion Changes on Sperm Head Size and Freezability in Ibex and Mouflon
title_sort influence of prolactin secretion changes on sperm head size and freezability in ibex and mouflon
publishDate 2022-06-08
url http://hdl.handle.net/10261/305117
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