Extracellular vesicles from oviductal and uterine fluids supplementation in sequential in vitro culture improves bovine embryo quality
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Language: | English |
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Springer Nature
2022-10-25
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Subjects: | Cattle, Cryopreservation, Embryo development, Exosomes, Lipid metabolism, Oviduct, Uterus, miRNAs, |
Online Access: | http://hdl.handle.net/10261/303946 http://dx.doi.org/10.13039/501100004299 http://dx.doi.org/10.13039/501100004837 https://api.elsevier.com/content/abstract/scopus_id/85140452525 |
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dig-inia-es-10261-3039462024-05-19T20:37:08Z Extracellular vesicles from oviductal and uterine fluids supplementation in sequential in vitro culture improves bovine embryo quality Leal, Cláudia Lima Verde Cañón-Beltrán, Karina Cajas, Yulia N. Hamdi, M. Yaryes, Aracelli Millán de la Blanca, María Gemma Beltrán Breña, Paula Mazzarella, Rosane da Silveira, Juliano C. Gutiérrez-Adán, Alfonso González, Encina M. Rizos, Dimitrios Ministerio de Ciencia e Innovación (España) Sao Paulo Research Foundation Secretaría de Educación Superior, Ciencia, Tecnología e Innovación (Ecuador) Cañón-Beltrán, Karina [0000-0002-0279-0857] Cajas, Yulia N. [0000-0001-9791-6733] Millán de la Blanca, María Gemma [0000-0002-2810-8510] Mazzarella, Rosane [0000-0001-6860-8897] Gutiérrez-Adán, Alfonso [0000-0001-9893-9179] González, Encina M. [0000-0002-6217-866X] Rizos, Dimitrios [0000-0001-6813-3940] Hamdi, M. [0000-0002-2792-8293] Cattle Cryopreservation Embryo development Exosomes Lipid metabolism Oviduct Uterus miRNAs 20 Pág. In vitro production of bovine embryos is a well-established technology, but the in vitro culture (IVC) system still warrants improvements, especially regarding embryo quality. This study aimed to evaluate the effect of extracellular vesicles (EVs) isolated from oviductal (OF) and uterine fluid (UF) in sequential IVC on the development and quality of bovine embryos. Zygotes were cultured in SOF supplemented with either BSA or EVs-depleted fetal calf serum (dFCS) in the presence (BSA-EV and dFCS-EV) or absence of EVs from OF (D1 to D4) and UF (D5 to D8), mimicking in vivo conditions. EVs from oviducts (early luteal phase) and uterine horns (mid-luteal phase) from slaughtered heifers were isolated by size exclusion chromatography. Blastocyst rate was recorded on days 7-8 and their quality was assessed based on lipid contents, mitochondrial activity and total cell numbers, as well as survival rate after vitrification. Relative mRNA abundance for lipid metabolism-related transcripts and levels of phosphorylated hormone-sensitive lipase (pHSL) proteins were also determined. Additionally, the expression levels of 383 miRNA in OF- and UF-EVs were assessed by qRT-PCR. This work was supported by the Spanish Ministry of Science and Innovation (PID2019-111641RB-I00 to DR, and RTI2018-093548-B-I00 to AGA); São Paulo Research Foundation, Brazil (FAPESP; #2017/20339-3 and CNPq-Brazil 304276/2018-9 to CLVL; #2019/04981-2 to RM; 2014/22887-0 and 2015/21829-9 to JCDS); Minciencias-Colombia Postdoctoral Fellowship (848-2019) to KC-B; Secretaria de Educación Superior, Ciencia y Tecnología e Innovación (SENESCYT-Ecuador) to YNC. Peer reviewed 2023-03-23T08:21:02Z 2023-03-23T08:21:02Z 2022-10-25 artículo http://purl.org/coar/resource_type/c_6501 Journal of Animal Science and Biotechnology 13: e116 (2022) 1674-9782 http://hdl.handle.net/10261/303946 10.1186/s40104-022-00763-7 2049-1891 http://dx.doi.org/10.13039/501100004299 http://dx.doi.org/10.13039/501100004837 36280872 2-s2.0-85140452525 https://api.elsevier.com/content/abstract/scopus_id/85140452525 en #PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-111641RB-I00/ES/VESICULAS EXTRACELULARES: MODULADORES DE LA INTERACCION MATERNO-EMBRIONARIA Y DE LA FERTILIDAD EN BOVINO/ info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-093548-B-I00/ES/ANALISIS FUNCIONAL DE ISOFORMAS DE ARNM RELACIONADAS CON DESARROLLO EMBRIONARIO Y FERTILIDAD/ Departamento de Reproducción Animal Publisher's version https://doi.org/10.1186/s40104-022-00763-7 Sí open Springer Nature |
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Cattle Cryopreservation Embryo development Exosomes Lipid metabolism Oviduct Uterus miRNAs Cattle Cryopreservation Embryo development Exosomes Lipid metabolism Oviduct Uterus miRNAs |
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Cattle Cryopreservation Embryo development Exosomes Lipid metabolism Oviduct Uterus miRNAs Cattle Cryopreservation Embryo development Exosomes Lipid metabolism Oviduct Uterus miRNAs Leal, Cláudia Lima Verde Cañón-Beltrán, Karina Cajas, Yulia N. Hamdi, M. Yaryes, Aracelli Millán de la Blanca, María Gemma Beltrán Breña, Paula Mazzarella, Rosane da Silveira, Juliano C. Gutiérrez-Adán, Alfonso González, Encina M. Rizos, Dimitrios Extracellular vesicles from oviductal and uterine fluids supplementation in sequential in vitro culture improves bovine embryo quality |
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20 Pág. |
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Ministerio de Ciencia e Innovación (España) |
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Ministerio de Ciencia e Innovación (España) Leal, Cláudia Lima Verde Cañón-Beltrán, Karina Cajas, Yulia N. Hamdi, M. Yaryes, Aracelli Millán de la Blanca, María Gemma Beltrán Breña, Paula Mazzarella, Rosane da Silveira, Juliano C. Gutiérrez-Adán, Alfonso González, Encina M. Rizos, Dimitrios |
format |
artículo |
topic_facet |
Cattle Cryopreservation Embryo development Exosomes Lipid metabolism Oviduct Uterus miRNAs |
author |
Leal, Cláudia Lima Verde Cañón-Beltrán, Karina Cajas, Yulia N. Hamdi, M. Yaryes, Aracelli Millán de la Blanca, María Gemma Beltrán Breña, Paula Mazzarella, Rosane da Silveira, Juliano C. Gutiérrez-Adán, Alfonso González, Encina M. Rizos, Dimitrios |
author_sort |
Leal, Cláudia Lima Verde |
title |
Extracellular vesicles from oviductal and uterine fluids supplementation in sequential in vitro culture improves bovine embryo quality |
title_short |
Extracellular vesicles from oviductal and uterine fluids supplementation in sequential in vitro culture improves bovine embryo quality |
title_full |
Extracellular vesicles from oviductal and uterine fluids supplementation in sequential in vitro culture improves bovine embryo quality |
title_fullStr |
Extracellular vesicles from oviductal and uterine fluids supplementation in sequential in vitro culture improves bovine embryo quality |
title_full_unstemmed |
Extracellular vesicles from oviductal and uterine fluids supplementation in sequential in vitro culture improves bovine embryo quality |
title_sort |
extracellular vesicles from oviductal and uterine fluids supplementation in sequential in vitro culture improves bovine embryo quality |
publisher |
Springer Nature |
publishDate |
2022-10-25 |
url |
http://hdl.handle.net/10261/303946 http://dx.doi.org/10.13039/501100004299 http://dx.doi.org/10.13039/501100004837 https://api.elsevier.com/content/abstract/scopus_id/85140452525 |
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