Tools for mosquito blood meal identification
11 Pág. Centro de Investigación en Sanidad Animal (CISA)
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Format: | capítulo de libro biblioteca |
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Wageningen Academic Publishers
2022-07-01
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Subjects: | Barcoding, COI gene, Cytochrome B, DNA, ELISA, Maldi-TOF, |
Online Access: | http://hdl.handle.net/10261/296965 https://api.elsevier.com/content/abstract/scopus_id/85134940982 |
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dig-inia-es-10261-2969652023-03-08T12:53:29Z Tools for mosquito blood meal identification Ecology and Control of Vector-borne Diseases: 7 Gutiérrez-López, Rafael Puente, Josué Martínez De La Figuerola Borras, Jordi Agencia Estatal de Investigación (España) Ministerio de Economía y Competitividad (España) European Commission Govern de les Illes Balears Gutiérrez-López, Rafael [0000-0003-0107-5357] Puente, Josué Martínez De La [0000-0001-8055-4115] Figuerola Borras, Jordi [0000-0002-4664-9011] Barcoding COI gene Cytochrome B DNA ELISA Maldi-TOF 11 Pág. Centro de Investigación en Sanidad Animal (CISA) The identification of the vertebrate blood meal sources of mosquitoes allows insight to better understand the dynamics of vector-borne pathogens. To do so, different approaches have been used, based on the use of the remains of blood present in the abdomen of recently engorged mosquito females. Among others, different authors have used serological techniques to the more recently developed approaches based on host DNA amplification or mass spectrometry. These methods have allowed researchers to identify the vertebrate hosts of mosquitoes accurately to the species level or, even, at the individual level, providing information on the relative importance of different mosquito species in the transmission of particular pathogens. These approaches have been especially relevant to reveal the contact rates between vectors, susceptible and competent hosts, and mosquito-borne pathogens, including zoonotic ones. Additionally, these methods have revealed important asymmetries in the attraction of mosquitoes towards different host species, allowing to identify key vertebrates for the amplification of some pathogens. This chapter reviews those tools most frequently used for the identification of the blood meals of mosquitoes in order to highlight the main advantages and limitations of these methodologies. This study was funded by the projects PGC2018-095704-B-I00 and PID2020-118205GB-I00 from the Spanish Ministry of Economy and Competition cofinanced by the European Regional Development Fund (FEDER). RGL was partially supported by a Margalida Comas contract from the Government of the Balearic Islands cofinanced by the European Regional Development Fund (FEDER) (REF-PD/038/2019) and a Juan de la Cierva-Formación contract. Peer reviewed 2023-03-08T12:53:28Z 2023-03-08T12:53:28Z 2022-07-01 capítulo de libro Ecology of diseases transmitted by mosquitoes to wildlife: 264 (2022) 978-90-8686-379-2 1875-0699 eISBN: 978-90-8686-931-2 http://hdl.handle.net/10261/296965 10.3920/978-90-8686-931-2_6 2-s2.0-85134940982 https://api.elsevier.com/content/abstract/scopus_id/85134940982 en #PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PGC2018-095704-B-I00/ES/IMPACTO DEL AMBIENTE Y LA CONDICION DE LOS VECTORES SOBRE LOS PATOGENOS TRANSMITIDOS POR VECTORES/ info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-118205GB-I00/ES/EFECTOS DE LA MICROBIOTA DEL MOSQUITO EN LA TRANSMISION DE PATOGENOS AVIARES/ Ecology and Control of Vector-Borne Diseases Publisher's version https://doi.org/10.3920/978-90-8686-931-2 Sí open Wageningen Academic Publishers |
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Barcoding COI gene Cytochrome B DNA ELISA Maldi-TOF Barcoding COI gene Cytochrome B DNA ELISA Maldi-TOF |
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Barcoding COI gene Cytochrome B DNA ELISA Maldi-TOF Barcoding COI gene Cytochrome B DNA ELISA Maldi-TOF Gutiérrez-López, Rafael Puente, Josué Martínez De La Figuerola Borras, Jordi Tools for mosquito blood meal identification |
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11 Pág.
Centro de Investigación en Sanidad Animal (CISA) |
author2 |
Agencia Estatal de Investigación (España) |
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Agencia Estatal de Investigación (España) Gutiérrez-López, Rafael Puente, Josué Martínez De La Figuerola Borras, Jordi |
format |
capítulo de libro |
topic_facet |
Barcoding COI gene Cytochrome B DNA ELISA Maldi-TOF |
author |
Gutiérrez-López, Rafael Puente, Josué Martínez De La Figuerola Borras, Jordi |
author_sort |
Gutiérrez-López, Rafael |
title |
Tools for mosquito blood meal identification |
title_short |
Tools for mosquito blood meal identification |
title_full |
Tools for mosquito blood meal identification |
title_fullStr |
Tools for mosquito blood meal identification |
title_full_unstemmed |
Tools for mosquito blood meal identification |
title_sort |
tools for mosquito blood meal identification |
publisher |
Wageningen Academic Publishers |
publishDate |
2022-07-01 |
url |
http://hdl.handle.net/10261/296965 https://api.elsevier.com/content/abstract/scopus_id/85134940982 |
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