Isolation and characterization of polymorphic nuclear microsatellite loci in Taxus baccata L
Seven polymorphic nuclear microsatellite markers for Taxus baccata L. (English yew) were developed using an enriched-library method. An additional polymorphic SSR was obtained by testing eight primer pairs from the congeneric species Taxus sumatrana. Mendelian inheritance for the seven Taxus baccata SSRs was proved by genotyping 17 individuals and 124 megagametophytes (conifer seed haploid tissue). A total of 96 individuals from 5 different populations (10-26 samples per population) were used to estimate genetic diversity parameters. High levels of genetic diversity, with values ranging from 0.533 to 0.929 (6-28 alleles per SSR) were found. No linkage disequilibrium between pairs of loci was detected. All loci but one showed significant departures from Hardy-Weinberg equilibrium. Excess of homozygosity was probably due to high inbreeding in English yew populations, an outcome of low effective population size and/or fragmented distribution. Highly polymorphic SSRs will be used to conduct population genetic studies at different geographical scales and to monitor gene flow. © 2008 Springer Science+Business Media B.V.
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2008
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Subjects: | Taxus baccata, Microsatellite markers, Genetic diversity, Inbreeding, |
Online Access: | http://hdl.handle.net/20.500.12792/5046 http://hdl.handle.net/10261/295297 |
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dig-inia-es-10261-2952972023-02-20T10:46:44Z Isolation and characterization of polymorphic nuclear microsatellite loci in Taxus baccata L Dubreuil, M. Sebastiani, F. Mayol, M. González-Martínez, S. C. Riba, M. Vendramin, G. G. Taxus baccata Microsatellite markers Genetic diversity Inbreeding Seven polymorphic nuclear microsatellite markers for Taxus baccata L. (English yew) were developed using an enriched-library method. An additional polymorphic SSR was obtained by testing eight primer pairs from the congeneric species Taxus sumatrana. Mendelian inheritance for the seven Taxus baccata SSRs was proved by genotyping 17 individuals and 124 megagametophytes (conifer seed haploid tissue). A total of 96 individuals from 5 different populations (10-26 samples per population) were used to estimate genetic diversity parameters. High levels of genetic diversity, with values ranging from 0.533 to 0.929 (6-28 alleles per SSR) were found. No linkage disequilibrium between pairs of loci was detected. All loci but one showed significant departures from Hardy-Weinberg equilibrium. Excess of homozygosity was probably due to high inbreeding in English yew populations, an outcome of low effective population size and/or fragmented distribution. Highly polymorphic SSRs will be used to conduct population genetic studies at different geographical scales and to monitor gene flow. © 2008 Springer Science+Business Media B.V. 2023-02-20T10:46:44Z 2023-02-20T10:46:44Z 2008 journal article Conservation Genetics 9: 1665-1668 (2008) 1566-0621 http://hdl.handle.net/20.500.12792/5046 http://hdl.handle.net/10261/295297 10.1007/s10592-008-9515-3 1572-9737 en none Springer |
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Taxus baccata Microsatellite markers Genetic diversity Inbreeding Taxus baccata Microsatellite markers Genetic diversity Inbreeding |
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Taxus baccata Microsatellite markers Genetic diversity Inbreeding Taxus baccata Microsatellite markers Genetic diversity Inbreeding Dubreuil, M. Sebastiani, F. Mayol, M. González-Martínez, S. C. Riba, M. Vendramin, G. G. Isolation and characterization of polymorphic nuclear microsatellite loci in Taxus baccata L |
description |
Seven polymorphic nuclear microsatellite markers for Taxus baccata L. (English yew) were developed using an enriched-library method. An additional polymorphic SSR was obtained by testing eight primer pairs from the congeneric species Taxus sumatrana. Mendelian inheritance for the seven Taxus baccata SSRs was proved by genotyping 17 individuals and 124 megagametophytes (conifer seed haploid tissue). A total of 96 individuals from 5 different populations (10-26 samples per population) were used to estimate genetic diversity parameters. High levels of genetic diversity, with values ranging from 0.533 to 0.929 (6-28 alleles per SSR) were found. No linkage disequilibrium between pairs of loci was detected. All loci but one showed significant departures from Hardy-Weinberg equilibrium. Excess of homozygosity was probably due to high inbreeding in English yew populations, an outcome of low effective population size and/or fragmented distribution. Highly polymorphic SSRs will be used to conduct population genetic studies at different geographical scales and to monitor gene flow. © 2008 Springer Science+Business Media B.V. |
format |
journal article |
topic_facet |
Taxus baccata Microsatellite markers Genetic diversity Inbreeding |
author |
Dubreuil, M. Sebastiani, F. Mayol, M. González-Martínez, S. C. Riba, M. Vendramin, G. G. |
author_facet |
Dubreuil, M. Sebastiani, F. Mayol, M. González-Martínez, S. C. Riba, M. Vendramin, G. G. |
author_sort |
Dubreuil, M. |
title |
Isolation and characterization of polymorphic nuclear microsatellite loci in Taxus baccata L |
title_short |
Isolation and characterization of polymorphic nuclear microsatellite loci in Taxus baccata L |
title_full |
Isolation and characterization of polymorphic nuclear microsatellite loci in Taxus baccata L |
title_fullStr |
Isolation and characterization of polymorphic nuclear microsatellite loci in Taxus baccata L |
title_full_unstemmed |
Isolation and characterization of polymorphic nuclear microsatellite loci in Taxus baccata L |
title_sort |
isolation and characterization of polymorphic nuclear microsatellite loci in taxus baccata l |
publisher |
Springer |
publishDate |
2008 |
url |
http://hdl.handle.net/20.500.12792/5046 http://hdl.handle.net/10261/295297 |
work_keys_str_mv |
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