High AN1 variability and interaction with basic helix-loop-helix co-factors related to anthocyanin biosynthesis in potato leaves
AN1 is a regulatory gene that promotes anthocyanin biosynthesis in potato tubers and encodes a R2R3 MYB transcription factor. However, no clear evidence implicates AN1 in anthocyanin production in leaves, where these pigments might enhance environmental stress tolerance. In our study we found that AN1 displays intraspecific sequence variability in both coding/non-coding regions and in the promoter, and that its expression is associated with high anthocyanin content in leaves of commercial potatoes. Expression analysis provided evidence that leaf pigmentation is associated to AN1 expression and that StJAF13 acts as putative AN1 co-regulator for anthocyanin gene expression in leaves of the red leaf variety 'Magenta Love,' while a concomitant expression of StbHLH1 may contribute to anthocyanin accumulation in leaves of 'Double Fun.' Yeast two-hybrid experiments confirmed that AN1 interacts with StbHLH1 and StJAF13 and the latter interaction was verified and localized in the cell nucleus by bimolecular fluorescence complementation assays. In addition, transgenic tobacco (Nicotiana tabacum) overexpressing a combination of either AN1 with StJAF13 or AN1 with StbHLH1 showed deeper purple pigmentation with respect to AN1 alone. This further confirmed AN1/StJAF13 and AN1/StbHLH1 interactions. Our findings demonstrate that the classical loci identified for potato leaf anthocyanin accumulation correspond to AN1 and may represent an important step to expand our knowledge on the molecular mechanisms underlying anthocyanin biosynthesis in different plant tissues. © 2014 The Authors. The Plant Journal © 2014 John Wiley & Sons Ltd.
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Format: | artículo biblioteca |
Language: | English |
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John Wiley & Sons
2014
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Subjects: | Solanum tuberosum, MYB transcription factor, Flavonoids, Regulatory complex, Allelic genotyping, Bimolecular fluorescence complementation, Yeast two hybrid assay, Transient and stable transformation, Nicotiana tabacum, |
Online Access: | http://hdl.handle.net/10261/295229 |
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dig-inia-es-10261-2952292023-11-05T09:42:11Z High AN1 variability and interaction with basic helix-loop-helix co-factors related to anthocyanin biosynthesis in potato leaves D'Amelia, V. Aversano, R. Batelli, G. Caruso, I. Moreno, M. C. Castro-Sanz, Ana B. Chiaiese, P. Fasano, C. Palomba, F. Carputo, D. Solanum tuberosum MYB transcription factor Flavonoids Regulatory complex Allelic genotyping Bimolecular fluorescence complementation Yeast two hybrid assay Transient and stable transformation Nicotiana tabacum AN1 is a regulatory gene that promotes anthocyanin biosynthesis in potato tubers and encodes a R2R3 MYB transcription factor. However, no clear evidence implicates AN1 in anthocyanin production in leaves, where these pigments might enhance environmental stress tolerance. In our study we found that AN1 displays intraspecific sequence variability in both coding/non-coding regions and in the promoter, and that its expression is associated with high anthocyanin content in leaves of commercial potatoes. Expression analysis provided evidence that leaf pigmentation is associated to AN1 expression and that StJAF13 acts as putative AN1 co-regulator for anthocyanin gene expression in leaves of the red leaf variety 'Magenta Love,' while a concomitant expression of StbHLH1 may contribute to anthocyanin accumulation in leaves of 'Double Fun.' Yeast two-hybrid experiments confirmed that AN1 interacts with StbHLH1 and StJAF13 and the latter interaction was verified and localized in the cell nucleus by bimolecular fluorescence complementation assays. In addition, transgenic tobacco (Nicotiana tabacum) overexpressing a combination of either AN1 with StJAF13 or AN1 with StbHLH1 showed deeper purple pigmentation with respect to AN1 alone. This further confirmed AN1/StJAF13 and AN1/StbHLH1 interactions. Our findings demonstrate that the classical loci identified for potato leaf anthocyanin accumulation correspond to AN1 and may represent an important step to expand our knowledge on the molecular mechanisms underlying anthocyanin biosynthesis in different plant tissues. © 2014 The Authors. The Plant Journal © 2014 John Wiley & Sons Ltd. 2023-02-20T10:46:04Z 2023-02-20T10:46:04Z 2014 artículo http://purl.org/coar/resource_type/c_6501 Plant Journal 80: 527-540 (2014) 0960-7412 http://hdl.handle.net/10261/295229 10.1111/tpj.12653 1365-313X en Centro de Biotecnología y Genómica de Plantas (CBGP) Sí none John Wiley & Sons |
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Solanum tuberosum MYB transcription factor Flavonoids Regulatory complex Allelic genotyping Bimolecular fluorescence complementation Yeast two hybrid assay Transient and stable transformation Nicotiana tabacum Solanum tuberosum MYB transcription factor Flavonoids Regulatory complex Allelic genotyping Bimolecular fluorescence complementation Yeast two hybrid assay Transient and stable transformation Nicotiana tabacum |
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Solanum tuberosum MYB transcription factor Flavonoids Regulatory complex Allelic genotyping Bimolecular fluorescence complementation Yeast two hybrid assay Transient and stable transformation Nicotiana tabacum Solanum tuberosum MYB transcription factor Flavonoids Regulatory complex Allelic genotyping Bimolecular fluorescence complementation Yeast two hybrid assay Transient and stable transformation Nicotiana tabacum D'Amelia, V. Aversano, R. Batelli, G. Caruso, I. Moreno, M. C. Castro-Sanz, Ana B. Chiaiese, P. Fasano, C. Palomba, F. Carputo, D. High AN1 variability and interaction with basic helix-loop-helix co-factors related to anthocyanin biosynthesis in potato leaves |
description |
AN1 is a regulatory gene that promotes anthocyanin biosynthesis in potato tubers and encodes a R2R3 MYB transcription factor. However, no clear evidence implicates AN1 in anthocyanin production in leaves, where these pigments might enhance environmental stress tolerance. In our study we found that AN1 displays intraspecific sequence variability in both coding/non-coding regions and in the promoter, and that its expression is associated with high anthocyanin content in leaves of commercial potatoes. Expression analysis provided evidence that leaf pigmentation is associated to AN1 expression and that StJAF13 acts as putative AN1 co-regulator for anthocyanin gene expression in leaves of the red leaf variety 'Magenta Love,' while a concomitant expression of StbHLH1 may contribute to anthocyanin accumulation in leaves of 'Double Fun.' Yeast two-hybrid experiments confirmed that AN1 interacts with StbHLH1 and StJAF13 and the latter interaction was verified and localized in the cell nucleus by bimolecular fluorescence complementation assays. In addition, transgenic tobacco (Nicotiana tabacum) overexpressing a combination of either AN1 with StJAF13 or AN1 with StbHLH1 showed deeper purple pigmentation with respect to AN1 alone. This further confirmed AN1/StJAF13 and AN1/StbHLH1 interactions. Our findings demonstrate that the classical loci identified for potato leaf anthocyanin accumulation correspond to AN1 and may represent an important step to expand our knowledge on the molecular mechanisms underlying anthocyanin biosynthesis in different plant tissues. © 2014 The Authors. The Plant Journal © 2014 John Wiley & Sons Ltd. |
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artículo |
topic_facet |
Solanum tuberosum MYB transcription factor Flavonoids Regulatory complex Allelic genotyping Bimolecular fluorescence complementation Yeast two hybrid assay Transient and stable transformation Nicotiana tabacum |
author |
D'Amelia, V. Aversano, R. Batelli, G. Caruso, I. Moreno, M. C. Castro-Sanz, Ana B. Chiaiese, P. Fasano, C. Palomba, F. Carputo, D. |
author_facet |
D'Amelia, V. Aversano, R. Batelli, G. Caruso, I. Moreno, M. C. Castro-Sanz, Ana B. Chiaiese, P. Fasano, C. Palomba, F. Carputo, D. |
author_sort |
D'Amelia, V. |
title |
High AN1 variability and interaction with basic helix-loop-helix co-factors related to anthocyanin biosynthesis in potato leaves |
title_short |
High AN1 variability and interaction with basic helix-loop-helix co-factors related to anthocyanin biosynthesis in potato leaves |
title_full |
High AN1 variability and interaction with basic helix-loop-helix co-factors related to anthocyanin biosynthesis in potato leaves |
title_fullStr |
High AN1 variability and interaction with basic helix-loop-helix co-factors related to anthocyanin biosynthesis in potato leaves |
title_full_unstemmed |
High AN1 variability and interaction with basic helix-loop-helix co-factors related to anthocyanin biosynthesis in potato leaves |
title_sort |
high an1 variability and interaction with basic helix-loop-helix co-factors related to anthocyanin biosynthesis in potato leaves |
publisher |
John Wiley & Sons |
publishDate |
2014 |
url |
http://hdl.handle.net/10261/295229 |
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