Molecular typing of Streptococcus suis isolates from Iberian pigs A comparison with isolates from common intensively-reared commercial pig breeds
The Iberian pig (IP) is a traditional Spanish breed variety of the domestic pig (. Sus scrofa domesticus) with high economic importance because of the value of the dry-cured products in national and international markets. The genetic characteristics of tonsillar and clinical Streptococcus suis isolates from the IP maintained under extensive or intensive management conditions were investigated. S.-suis isolates from IP pigs were compared with S.-suis isolates from intensively-farmed pigs of common breeds (CBP). S.-suis was isolated from 48.4% of the IP tonsils examined, indicating wide distribution among IP pigs.Serotypes 1 (9.4%), 2 (8.6%) and 9 (7%) were the most commonly found, although a high percentage of S.-suis isolates were not typeable by coagglutination testing. No significant differences in carrier rates or serotype diversity were observed between management systems, indicating that intensive farming does not influence S.-suis colonisation. Both pulsed-field gel electrophoresis and multiple-locus variable number tandem repeat analysis showed a serotype-based distribution of S.-suis IP isolates. Serotypes 1 and 2 S.-suis isolates were grouped in the same cluster, whereas isolates of serotypes 9 and 7 were assigned to another cluster. All clinical and most tonsillar serotype 2 IP isolates were assigned to sequence type 1 (ST1) and exhibited the virulence genotype mrp+/epf+/sly+, indicating a high distribution of this genetic lineage among IP as well as a population of serotype 2 common to IPs and CBPs. The only clinical isolate of serotype 9 from IP was assigned to ST123, a sequence type associated with clinical isolates in CBPs in Spain. © 2014 Elsevier Ltd.
| Main Authors: | , , , , , , |
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| Format: | journal article biblioteca |
| Language: | English |
| Published: |
Elsevier
2014
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| Subjects: | Iberian pigs, Molecular characterisation, Streptococcus suis, |
| Online Access: | http://hdl.handle.net/20.500.12792/5459 http://hdl.handle.net/10261/295018 |
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| Summary: | The Iberian pig (IP) is a traditional Spanish breed variety of the domestic pig (. Sus scrofa domesticus) with high economic importance because of the value of the dry-cured products in national and international markets. The genetic characteristics of tonsillar and clinical Streptococcus suis isolates from the IP maintained under extensive or intensive management conditions were investigated. S.-suis isolates from IP pigs were compared with S.-suis isolates from intensively-farmed pigs of common breeds (CBP). S.-suis was isolated from 48.4% of the IP tonsils examined, indicating wide distribution among IP pigs.Serotypes 1 (9.4%), 2 (8.6%) and 9 (7%) were the most commonly found, although a high percentage of S.-suis isolates were not typeable by coagglutination testing. No significant differences in carrier rates or serotype diversity were observed between management systems, indicating that intensive farming does not influence S.-suis colonisation. Both pulsed-field gel electrophoresis and multiple-locus variable number tandem repeat analysis showed a serotype-based distribution of S.-suis IP isolates. Serotypes 1 and 2 S.-suis isolates were grouped in the same cluster, whereas isolates of serotypes 9 and 7 were assigned to another cluster. All clinical and most tonsillar serotype 2 IP isolates were assigned to sequence type 1 (ST1) and exhibited the virulence genotype mrp+/epf+/sly+, indicating a high distribution of this genetic lineage among IP as well as a population of serotype 2 common to IPs and CBPs. The only clinical isolate of serotype 9 from IP was assigned to ST123, a sequence type associated with clinical isolates in CBPs in Spain. © 2014 Elsevier Ltd. |
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