Incidence and transmission of Faba bean necrotic yellows virus (FBNYV) in Spain
Information on the distribution of Faba bean necrotic yellows virus (FBNYV) in Spain was gathered by sampling different legume crops (faba bean 'Muchamiel', forage faba bean, pea and chickpea) in four regions (Murcia, Andalucía, Castilla y León, and Extremadura). The virus was detected by TAS-ELISA in faba bean 'Muchamiel' and peas only in the Murcia region. A portion of 272 bp within the C5 component of a FBNYV from Murcia was amplified by IC-PCR and directly sequenced. The Spanish FBNYV was 93.75% identical to two previously sequenced isolates of FBNYV from Syria and Egypt. Periodic samplings of faba bean 'Muchamiel' over one growing season (Oct. 1999 to Jan. 2000) in Murcia provided no clear evidence of any spread of the disease either within or beyond the affected fields. Mixed infections of FBNYV and Tomato spotted wilt virus (TSWV), Bean leaf roll virus (BLRV) and Bean yellow mosaic virus (BYMV) were commonly observed. The necrotic symptoms developed on the leaf borders were more pronounced in these mixed infections. Transmission experiments using a Spanish isolate as inoculum source and the aphid species Acyrthosiphon pisum and Aphis craccivora showed that both are efficient FBNYV vectors. In these experiments, the faba bean cvs. 'Aguadulce', 'Muchamiel' and 'Valenciana' were all susceptible to FBNYV.
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Format: | journal article biblioteca |
Language: | English |
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CSIC - Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA)
2006
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Subjects: | IC-PCR, Legume virus, Mixed infections, Sequencing, TAS-ELISA, Viruliferous aphids, |
Online Access: | http://hdl.handle.net/20.500.12792/5354 http://hdl.handle.net/10261/294975 |
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dig-inia-es-10261-2949752023-02-20T10:43:51Z Incidence and transmission of Faba bean necrotic yellows virus (FBNYV) in Spain Ortiz, V. Navarro, E. Castro, S. Carazo, G. Romero, J. IC-PCR Legume virus Mixed infections Sequencing TAS-ELISA Viruliferous aphids Information on the distribution of Faba bean necrotic yellows virus (FBNYV) in Spain was gathered by sampling different legume crops (faba bean 'Muchamiel', forage faba bean, pea and chickpea) in four regions (Murcia, Andalucía, Castilla y León, and Extremadura). The virus was detected by TAS-ELISA in faba bean 'Muchamiel' and peas only in the Murcia region. A portion of 272 bp within the C5 component of a FBNYV from Murcia was amplified by IC-PCR and directly sequenced. The Spanish FBNYV was 93.75% identical to two previously sequenced isolates of FBNYV from Syria and Egypt. Periodic samplings of faba bean 'Muchamiel' over one growing season (Oct. 1999 to Jan. 2000) in Murcia provided no clear evidence of any spread of the disease either within or beyond the affected fields. Mixed infections of FBNYV and Tomato spotted wilt virus (TSWV), Bean leaf roll virus (BLRV) and Bean yellow mosaic virus (BYMV) were commonly observed. The necrotic symptoms developed on the leaf borders were more pronounced in these mixed infections. Transmission experiments using a Spanish isolate as inoculum source and the aphid species Acyrthosiphon pisum and Aphis craccivora showed that both are efficient FBNYV vectors. In these experiments, the faba bean cvs. 'Aguadulce', 'Muchamiel' and 'Valenciana' were all susceptible to FBNYV. 2023-02-20T10:43:51Z 2023-02-20T10:43:51Z 2006 journal article Spanish Journal of Agricultural Research 4(3): 255-260 (2006) 1695-971X http://hdl.handle.net/20.500.12792/5354 http://hdl.handle.net/10261/294975 10.5424/sjar/2006043-200 2171-9292 en open CSIC - Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA) |
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IC-PCR Legume virus Mixed infections Sequencing TAS-ELISA Viruliferous aphids IC-PCR Legume virus Mixed infections Sequencing TAS-ELISA Viruliferous aphids |
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IC-PCR Legume virus Mixed infections Sequencing TAS-ELISA Viruliferous aphids IC-PCR Legume virus Mixed infections Sequencing TAS-ELISA Viruliferous aphids Ortiz, V. Navarro, E. Castro, S. Carazo, G. Romero, J. Incidence and transmission of Faba bean necrotic yellows virus (FBNYV) in Spain |
description |
Information on the distribution of Faba bean necrotic yellows virus (FBNYV) in Spain was gathered by sampling different legume crops (faba bean 'Muchamiel', forage faba bean, pea and chickpea) in four regions (Murcia, Andalucía, Castilla y León, and Extremadura). The virus was detected by TAS-ELISA in faba bean 'Muchamiel' and peas only in the Murcia region. A portion of 272 bp within the C5 component of a FBNYV from Murcia was amplified by IC-PCR and directly sequenced. The Spanish FBNYV was 93.75% identical to two previously sequenced isolates of FBNYV from Syria and Egypt. Periodic samplings of faba bean 'Muchamiel' over one growing season (Oct. 1999 to Jan. 2000) in Murcia provided no clear evidence of any spread of the disease either within or beyond the affected fields. Mixed infections of FBNYV and Tomato spotted wilt virus (TSWV), Bean leaf roll virus (BLRV) and Bean yellow mosaic virus (BYMV) were commonly observed. The necrotic symptoms developed on the leaf borders were more pronounced in these mixed infections. Transmission experiments using a Spanish isolate as inoculum source and the aphid species Acyrthosiphon pisum and Aphis craccivora showed that both are efficient FBNYV vectors. In these experiments, the faba bean cvs. 'Aguadulce', 'Muchamiel' and 'Valenciana' were all susceptible to FBNYV. |
format |
journal article |
topic_facet |
IC-PCR Legume virus Mixed infections Sequencing TAS-ELISA Viruliferous aphids |
author |
Ortiz, V. Navarro, E. Castro, S. Carazo, G. Romero, J. |
author_facet |
Ortiz, V. Navarro, E. Castro, S. Carazo, G. Romero, J. |
author_sort |
Ortiz, V. |
title |
Incidence and transmission of Faba bean necrotic yellows virus (FBNYV) in Spain |
title_short |
Incidence and transmission of Faba bean necrotic yellows virus (FBNYV) in Spain |
title_full |
Incidence and transmission of Faba bean necrotic yellows virus (FBNYV) in Spain |
title_fullStr |
Incidence and transmission of Faba bean necrotic yellows virus (FBNYV) in Spain |
title_full_unstemmed |
Incidence and transmission of Faba bean necrotic yellows virus (FBNYV) in Spain |
title_sort |
incidence and transmission of faba bean necrotic yellows virus (fbnyv) in spain |
publisher |
CSIC - Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA) |
publishDate |
2006 |
url |
http://hdl.handle.net/20.500.12792/5354 http://hdl.handle.net/10261/294975 |
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