In situ random primer extension of metaphase chromosomes
We have developed a technique of random primer extension of fixed chromosomes that is applicable to both mouse and man. Human chromosomes are not homogeneously labeled with this technique; those regions corresponding to R-bands appear to be more sensitive than those identified as G-bands, whereas centromeric regions are not labeled. These results not only corroborate specific structural differences between distinct regions of mammalian genomes but also open up the possibility of assays with specific primers to test whether primer extension is useful for the identification of genes and families of sequences on chromosomes.
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S. Karger AG
1990
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| Online Access: | http://hdl.handle.net/20.500.12792/5111 http://hdl.handle.net/10261/294893 |
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dig-inia-es-10261-2948932023-02-20T10:43:01Z In situ random primer extension of metaphase chromosomes García de la Vega, C. Martínez Zapater, J. M. López-Fernández, C. Goyanes, V. Mezzanotte, R. Gosálvez, J. We have developed a technique of random primer extension of fixed chromosomes that is applicable to both mouse and man. Human chromosomes are not homogeneously labeled with this technique; those regions corresponding to R-bands appear to be more sensitive than those identified as G-bands, whereas centromeric regions are not labeled. These results not only corroborate specific structural differences between distinct regions of mammalian genomes but also open up the possibility of assays with specific primers to test whether primer extension is useful for the identification of genes and families of sequences on chromosomes. 2023-02-20T10:43:01Z 2023-02-20T10:43:01Z 1990 journal article Cytogenet Cell Genet 53: 211-212 (1990) 1424-8581 http://hdl.handle.net/20.500.12792/5111 http://hdl.handle.net/10261/294893 10.1159/000132932 1424-859X en none S. Karger AG |
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ES |
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Biblioteca del INIA España |
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English |
| description |
We have developed a technique of random primer extension of fixed chromosomes that is applicable to both mouse and man. Human chromosomes are not homogeneously labeled with this technique; those regions corresponding to R-bands appear to be more sensitive than those identified as G-bands, whereas centromeric regions are not labeled. These results not only corroborate specific structural differences between distinct regions of mammalian genomes but also open up the possibility of assays with specific primers to test whether primer extension is useful for the identification of genes and families of sequences on chromosomes. |
| format |
journal article |
| author |
García de la Vega, C. Martínez Zapater, J. M. López-Fernández, C. Goyanes, V. Mezzanotte, R. Gosálvez, J. |
| spellingShingle |
García de la Vega, C. Martínez Zapater, J. M. López-Fernández, C. Goyanes, V. Mezzanotte, R. Gosálvez, J. In situ random primer extension of metaphase chromosomes |
| author_facet |
García de la Vega, C. Martínez Zapater, J. M. López-Fernández, C. Goyanes, V. Mezzanotte, R. Gosálvez, J. |
| author_sort |
García de la Vega, C. |
| title |
In situ random primer extension of metaphase chromosomes |
| title_short |
In situ random primer extension of metaphase chromosomes |
| title_full |
In situ random primer extension of metaphase chromosomes |
| title_fullStr |
In situ random primer extension of metaphase chromosomes |
| title_full_unstemmed |
In situ random primer extension of metaphase chromosomes |
| title_sort |
in situ random primer extension of metaphase chromosomes |
| publisher |
S. Karger AG |
| publishDate |
1990 |
| url |
http://hdl.handle.net/20.500.12792/5111 http://hdl.handle.net/10261/294893 |
| work_keys_str_mv |
AT garciadelavegac insiturandomprimerextensionofmetaphasechromosomes AT martinezzapaterjm insiturandomprimerextensionofmetaphasechromosomes AT lopezfernandezc insiturandomprimerextensionofmetaphasechromosomes AT goyanesv insiturandomprimerextensionofmetaphasechromosomes AT mezzanotter insiturandomprimerextensionofmetaphasechromosomes AT gosalvezj insiturandomprimerextensionofmetaphasechromosomes |
| _version_ |
1767603671402545152 |