Plant regeneration through somatic embryogenesis in Quercus suber
Cork oak (Quercus suber L.) zygotic embryos, endosperm and ovules were treated with different concentrations of 2,4‐D for induction of somatic embryos. Plant material was collected during the embryo development season, from June to September. Immature embryos proved to be the most reactive initial explant. Callus and somatic embryos developed a few weeks after the beginning of the 2,4‐D treatment. For embryo development experiments, different growth regulators and cold and desiccation treatments were tested. Cold storage of somatic embryos matured in vitro at 5°C was the best treatment for breaking dormancy. Copyright © 1992, Wiley Blackwell. All rights reserved
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Main Authors: | Bueno, M. A., Astorga, R., Manzanera, J. A. |
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Format: | journal article biblioteca |
Language: | English |
Published: |
Wiley
1992
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Subjects: | Cork oak, Plant regeneration, Quercus suber, Somatic embryogenesis, Tissue culture, |
Online Access: | http://hdl.handle.net/20.500.12792/4922 http://hdl.handle.net/10261/294814 |
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